Abstract
Despite advances in understanding the metabolism of Pseudomonas putida
KT2440, a promising bacterial host for producing valuable chemicals
from plant-derived feedstocks, a strain capable of producing free fatty
acid-derived chemicals has not been developed. Guided by functional
genomics, we engineered P. putida to produce medium- and
long-chain free fatty acids (FFAs) to titers of up to 670 mg/L.
Additionally, by taking advantage of the varying substrate preferences
of paralogous native fatty acyl-CoA ligases, we employed a strategy to
control FFA chain length that resulted in a P. putida strain
specialized in producing medium-chain FFAs. Finally, we demonstrate the
production of oleochemicals in these strains by synthesizing
medium-chain fatty acid methyl esters, compounds useful as biodiesel
blending agents, in various media including sorghum hydrolysate at
titers greater than 300 mg/L. This work paves the road to produce
high-value oleochemicals and biofuels from cheap feedstocks, such as
plant biomass, using this host.
Original language | English |
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Article number | 1363 |
Journal | Communications Biology |
Volume | 5 |
Issue number | 1 |
Number of pages | 12 |
ISSN | 2399-3642 |
DOIs | |
Publication status | Published - 2022 |