@inbook{982c2704e1a44a779f7721952646b22a,
title = "Engineering Gram-negative microbial cell factories using transposon vectors",
abstract = "The construction of microbial cell factories {\`a} la carte largely depends on specialized molecular biology and synthetic biology tools needed to reprogram bacteria for modifying their existing functions or for bestowing them with new-to-Nature tasks. In this chapter, we document the use of a series of broad-host-range mini-Tn5 vectors for the delivery of gene(s) into the chromosome of Gram-negative bacteria and for the generation of saturated, random mutagenesis libraries for studies of gene function. The application of these tailored mini-transposon vectors, which could also be used for chromosomal engineering of a wide variety of Gram-negative microorganisms, is demonstrated in the platform environmental bacterium Pseudomonas putida KT2440.",
keywords = "Mini-transposon, Tn 5 transposon, Pseudomonas putida, Escherichia coli, Synthetic biology, Metabolic engineering, Microbial cell factory, Genome editing",
author = "Esteban Mart{\'i}nez-Garc{\'i}a and Tom{\'a}s Aparicio and {de Lorenzo}, Victor and Nikel, {Pablo Ivan}",
year = "2016",
doi = "10.1007/978-1-4939-6472-7_18",
language = "English",
isbn = "978-1-4939-6470-3",
series = "Methods in Molecular Biology",
publisher = "Springer New York",
pages = "273--293",
booktitle = "In Vitro Mutagenesis",
address = "United States",
}