Engineering complex tissue-like microgel arrays for evaluating stem cell differentiation

Enrico Guermani, Hossein Shaki, Soumyaranjan Mohanty, Mehdi Mehrali, Ayyoob Arpanaei, Akhilesh K. Gaharwar, Alireza Dolatshahi-Pirouz

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    Abstract

    Development of tissue engineering scaffolds with native-like biology and microarchitectures is a prerequisite for stem cell mediated generation of off-the-shelf-tissues. So far, the field of tissue engineering has not full-filled its grand potential of engineering such combinatorial scaffolds for engineering functional tissues. This is primarily due to the many challenges associated with finding the right microarchitectures and ECM compositions for optimal tissue regeneration. Here, we have developed a new microgel array to address this grand challenge through robotic printing of complex stem cell-laden microgel arrays. The developed microgel array platform consisted of various microgel environments that where composed of native-like cellular microarchitectures resembling vascularized and bone marrow tissue architectures. The feasibility of our array system was demonstrated through localized cell spreading and osteogenic differentiation of human mesenchymal stem cells (hMSCs) into complex tissue-like structures. In summary, we have developed a tissue-like microgel array for evaluating stem cell differentiation within complex and heterogeneous cell microenvironments. We anticipate that the developed platform will be used for high-throughput identification of combinatorial and native-like scaffolds for tissue engineering of functional organs.
    Original languageEnglish
    JournalScientific Reports
    Volume6
    Pages (from-to)30445
    Number of pages1
    ISSN2045-2322
    DOIs
    Publication statusPublished - 2016

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