Engineered CHO cells for production of diverse, homogeneous glycoproteins

Zhang Yang, Shengjun Wang, Adnan Halim, Morten Alder Schulz, Morten Frodin, Shamim H. Rahman, Malene Bech Vester-Christensen, Carsten Behrens, Claus Kristensen, Sergey Y. Vakhrushev, Eric Paul Bennett, Hans H. Wandall, Henrik Clausen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like alpha 2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.
Original languageEnglish
JournalNature Biotechnology
Volume33
Issue number8
Pages (from-to)842-844
Number of pages3
ISSN1087-0156
DOIs
Publication statusPublished - 2015

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