Engineer medium and feed for modulating N-glycosylation of recombinant protein production in CHO cell culture

Yuzhou Fan, Helene Faustrup Kildegaard, Mikael Rørdam Andersen

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Chinese hamster ovary (CHO) cells have become the primary expression system for the production of complex recombinant proteins due to their long-term success in industrial scale production and generating appropriate protein N-glycans similar to that of humans. Control and optimization of protein N-glycosylation is crucial, as the structure of N-glycans can largely influence both biological and physicochemical properties of recombinant proteins. Protein N-glycosylation in CHO cell culture can be controlled and tuned by engineering medium, feed, culture process, as well as genetic elements of the cell. In this chapter, we will focus on how to carry out experiments for N-glycosylation modulation through medium and feed optimization. The workflow and typical methods involved in the experiment process will be presented.
Original languageEnglish
Title of host publicationHeterologous Protein Production in CHO cells
PublisherSpringer
Publication date2017
Pages209-226
Chapter14
DOIs
Publication statusPublished - 2017
SeriesMethods in Molecular Biology
Volume1603
ISSN1064-3745

Keywords

  • Chinese hamster ovary cells
  • N-Glycosylation
  • Medium and feed optimization
  • Fed-batch culture

Cite this

Fan, Y., Kildegaard, H. F., & Andersen, M. R. (2017). Engineer medium and feed for modulating N-glycosylation of recombinant protein production in CHO cell culture. In Heterologous Protein Production in CHO cells (pp. 209-226). Springer. Methods in Molecular Biology, Vol.. 1603 https://doi.org/10.1007/978-1-4939-6972-2_14
Fan, Yuzhou ; Kildegaard, Helene Faustrup ; Andersen, Mikael Rørdam. / Engineer medium and feed for modulating N-glycosylation of recombinant protein production in CHO cell culture. Heterologous Protein Production in CHO cells. Springer, 2017. pp. 209-226 (Methods in Molecular Biology, Vol. 1603).
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Fan, Y, Kildegaard, HF & Andersen, MR 2017, Engineer medium and feed for modulating N-glycosylation of recombinant protein production in CHO cell culture. in Heterologous Protein Production in CHO cells. Springer, Methods in Molecular Biology, vol. 1603, pp. 209-226. https://doi.org/10.1007/978-1-4939-6972-2_14

Engineer medium and feed for modulating N-glycosylation of recombinant protein production in CHO cell culture. / Fan, Yuzhou; Kildegaard, Helene Faustrup; Andersen, Mikael Rørdam.

Heterologous Protein Production in CHO cells. Springer, 2017. p. 209-226 (Methods in Molecular Biology, Vol. 1603).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

TY - CHAP

T1 - Engineer medium and feed for modulating N-glycosylation of recombinant protein production in CHO cell culture

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Y1 - 2017

N2 - Chinese hamster ovary (CHO) cells have become the primary expression system for the production of complex recombinant proteins due to their long-term success in industrial scale production and generating appropriate protein N-glycans similar to that of humans. Control and optimization of protein N-glycosylation is crucial, as the structure of N-glycans can largely influence both biological and physicochemical properties of recombinant proteins. Protein N-glycosylation in CHO cell culture can be controlled and tuned by engineering medium, feed, culture process, as well as genetic elements of the cell. In this chapter, we will focus on how to carry out experiments for N-glycosylation modulation through medium and feed optimization. The workflow and typical methods involved in the experiment process will be presented.

AB - Chinese hamster ovary (CHO) cells have become the primary expression system for the production of complex recombinant proteins due to their long-term success in industrial scale production and generating appropriate protein N-glycans similar to that of humans. Control and optimization of protein N-glycosylation is crucial, as the structure of N-glycans can largely influence both biological and physicochemical properties of recombinant proteins. Protein N-glycosylation in CHO cell culture can be controlled and tuned by engineering medium, feed, culture process, as well as genetic elements of the cell. In this chapter, we will focus on how to carry out experiments for N-glycosylation modulation through medium and feed optimization. The workflow and typical methods involved in the experiment process will be presented.

KW - Chinese hamster ovary cells

KW - N-Glycosylation

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KW - Fed-batch culture

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BT - Heterologous Protein Production in CHO cells

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Fan Y, Kildegaard HF, Andersen MR. Engineer medium and feed for modulating N-glycosylation of recombinant protein production in CHO cell culture. In Heterologous Protein Production in CHO cells. Springer. 2017. p. 209-226. (Methods in Molecular Biology, Vol. 1603). https://doi.org/10.1007/978-1-4939-6972-2_14