Energy Starvation Induces a Cell Cycle Arrest in Escherichia coli by Triggering Degradation of the DnaA Initiator Protein

Godefroid Charbon*, Belén Mendoza-Chamizo, Christopher Campion, Xiaobo Li, Peter Ruhdal Jensen, Jakob Frimodt-Møller, Anders Løbner-Olesen

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

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Abstract

During steady-state Escherichia coli growth, the amount and activity of the initiator protein, DnaA, controls chromosome replication tightly so that initiation only takes place once per origin in each cell cycle, regardless of growth conditions. However, little is known about the mechanisms involved during transitions from one environmental condition to another or during starvation stress. ATP depletion is one of the consequences of long-term carbon starvation. Here we show that DnaA is degraded in ATP-depleted cells. A chromosome replication initiation block is apparent in such cells as no new rounds of DNA replication are initiated while replication events that have already started proceed to completion.

Original languageEnglish
Article number629953
JournalFrontiers in Molecular Biosciences
Volume8
Number of pages9
ISSN2296-889X
DOIs
Publication statusPublished - 2021

Keywords

  • chromosome replication
  • degradation
  • DnaA protein
  • energy starvation
  • Escherichia coli
  • initiation

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