Endocrine disrupting effects in rats perinatally exposed to a dietary relevant mixture of phytoestrogens

Julie Boberg, Karen Mandrup, Pernille Rosenskjold Jacobsen, Louise Krag Isling, Niels Hadrup, Line Olrik Berthelsen, Anders Elleby Engell-Kofoed, Maria Kristina Kiersgaard, Anne Marie Vinggaard, Ulla Hass, Christine Lydia Nellemann

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Dietary phytoestrogens may prevent certain human diseases, but endocrine activity has been reported in animal studies. Sprague-Dawley rats were exposed perinatally to a 1-, 10- or 100-fold “high human dietary intake” mixture of 12 phytoestrogens consisting of mainly the lignan secoisolarici resinol and the isoflavones genistein and daidzein.This mixture induced persistent adverse effects, as adult male mammary glands showed hypertrophic growth. A reduced anogenital distance in newborn males indicated an anti-androgenic mode of action. Testosterone levels, testis and prostate weights, and expression of selected genes in testis and prostate were unaffected. Decreased serum estradiol was seen in genistein-exposed dams. This study indicated adverse effects at high intake levels in rats, but does not provide evidence for risk of phytoestrogen-mediated endocrine disruption at normal human dietary consumption levels. Further studies are warranted to increase the knowledge upon which risk assessment on dietary phytoestrogen exposure during pregnancy and infancy is based.
Original languageEnglish
JournalReproductive Toxicology
Volume40
Pages (from-to)41-51
ISSN0890-6238
DOIs
Publication statusPublished - 2013

Keywords

  • Mammary
  • Histology
  • Daidzein
  • Genistein
  • Secoisolarici resinol
  • Lignans
  • Diet
  • Anti-androgenic

Cite this

Boberg, Julie ; Mandrup, Karen ; Jacobsen, Pernille Rosenskjold ; Isling, Louise Krag ; Hadrup, Niels ; Berthelsen, Line Olrik ; Engell-Kofoed, Anders Elleby ; Kiersgaard, Maria Kristina ; Vinggaard, Anne Marie ; Hass, Ulla ; Nellemann, Christine Lydia. / Endocrine disrupting effects in rats perinatally exposed to a dietary relevant mixture of phytoestrogens. In: Reproductive Toxicology. 2013 ; Vol. 40. pp. 41-51.
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title = "Endocrine disrupting effects in rats perinatally exposed to a dietary relevant mixture of phytoestrogens",
abstract = "Dietary phytoestrogens may prevent certain human diseases, but endocrine activity has been reported in animal studies. Sprague-Dawley rats were exposed perinatally to a 1-, 10- or 100-fold “high human dietary intake” mixture of 12 phytoestrogens consisting of mainly the lignan secoisolarici resinol and the isoflavones genistein and daidzein.This mixture induced persistent adverse effects, as adult male mammary glands showed hypertrophic growth. A reduced anogenital distance in newborn males indicated an anti-androgenic mode of action. Testosterone levels, testis and prostate weights, and expression of selected genes in testis and prostate were unaffected. Decreased serum estradiol was seen in genistein-exposed dams. This study indicated adverse effects at high intake levels in rats, but does not provide evidence for risk of phytoestrogen-mediated endocrine disruption at normal human dietary consumption levels. Further studies are warranted to increase the knowledge upon which risk assessment on dietary phytoestrogen exposure during pregnancy and infancy is based.",
keywords = "Mammary, Histology, Daidzein, Genistein, Secoisolarici resinol, Lignans, Diet, Anti-androgenic",
author = "Julie Boberg and Karen Mandrup and Jacobsen, {Pernille Rosenskjold} and Isling, {Louise Krag} and Niels Hadrup and Berthelsen, {Line Olrik} and Engell-Kofoed, {Anders Elleby} and Kiersgaard, {Maria Kristina} and Vinggaard, {Anne Marie} and Ulla Hass and Nellemann, {Christine Lydia}",
year = "2013",
doi = "10.1016/j.reprotox.2013.05.014",
language = "English",
volume = "40",
pages = "41--51",
journal = "Reproductive Toxicology",
issn = "0890-6238",
publisher = "Elsevier",

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Endocrine disrupting effects in rats perinatally exposed to a dietary relevant mixture of phytoestrogens. / Boberg, Julie; Mandrup, Karen; Jacobsen, Pernille Rosenskjold; Isling, Louise Krag; Hadrup, Niels; Berthelsen, Line Olrik; Engell-Kofoed, Anders Elleby; Kiersgaard, Maria Kristina; Vinggaard, Anne Marie; Hass, Ulla; Nellemann, Christine Lydia.

In: Reproductive Toxicology, Vol. 40, 2013, p. 41-51.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Endocrine disrupting effects in rats perinatally exposed to a dietary relevant mixture of phytoestrogens

AU - Boberg, Julie

AU - Mandrup, Karen

AU - Jacobsen, Pernille Rosenskjold

AU - Isling, Louise Krag

AU - Hadrup, Niels

AU - Berthelsen, Line Olrik

AU - Engell-Kofoed, Anders Elleby

AU - Kiersgaard, Maria Kristina

AU - Vinggaard, Anne Marie

AU - Hass, Ulla

AU - Nellemann, Christine Lydia

PY - 2013

Y1 - 2013

N2 - Dietary phytoestrogens may prevent certain human diseases, but endocrine activity has been reported in animal studies. Sprague-Dawley rats were exposed perinatally to a 1-, 10- or 100-fold “high human dietary intake” mixture of 12 phytoestrogens consisting of mainly the lignan secoisolarici resinol and the isoflavones genistein and daidzein.This mixture induced persistent adverse effects, as adult male mammary glands showed hypertrophic growth. A reduced anogenital distance in newborn males indicated an anti-androgenic mode of action. Testosterone levels, testis and prostate weights, and expression of selected genes in testis and prostate were unaffected. Decreased serum estradiol was seen in genistein-exposed dams. This study indicated adverse effects at high intake levels in rats, but does not provide evidence for risk of phytoestrogen-mediated endocrine disruption at normal human dietary consumption levels. Further studies are warranted to increase the knowledge upon which risk assessment on dietary phytoestrogen exposure during pregnancy and infancy is based.

AB - Dietary phytoestrogens may prevent certain human diseases, but endocrine activity has been reported in animal studies. Sprague-Dawley rats were exposed perinatally to a 1-, 10- or 100-fold “high human dietary intake” mixture of 12 phytoestrogens consisting of mainly the lignan secoisolarici resinol and the isoflavones genistein and daidzein.This mixture induced persistent adverse effects, as adult male mammary glands showed hypertrophic growth. A reduced anogenital distance in newborn males indicated an anti-androgenic mode of action. Testosterone levels, testis and prostate weights, and expression of selected genes in testis and prostate were unaffected. Decreased serum estradiol was seen in genistein-exposed dams. This study indicated adverse effects at high intake levels in rats, but does not provide evidence for risk of phytoestrogen-mediated endocrine disruption at normal human dietary consumption levels. Further studies are warranted to increase the knowledge upon which risk assessment on dietary phytoestrogen exposure during pregnancy and infancy is based.

KW - Mammary

KW - Histology

KW - Daidzein

KW - Genistein

KW - Secoisolarici resinol

KW - Lignans

KW - Diet

KW - Anti-androgenic

U2 - 10.1016/j.reprotox.2013.05.014

DO - 10.1016/j.reprotox.2013.05.014

M3 - Journal article

VL - 40

SP - 41

EP - 51

JO - Reproductive Toxicology

JF - Reproductive Toxicology

SN - 0890-6238

ER -