Encoding arbitrary grey-level optical landscapes for trapping and manipulation using GPC

Carlo Amadeo Alonzo, Peter John Rodrigo, Darwin Palima, Jesper Glückstad

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review


    With the aid of phase-only spatial light modulators (SLM), generalized phase contrast (GPC) has been applied with great success to the projection of binary light patterns through arbitrary-NA microscope objectives for real-time three-dimensional manipulation of microscopic particles. Here, we review the analysis of the GPC method with emphasis on efficiently producing speckle-free two-dimensional grey-level light Patterns. Numerical simulations are applied to construct 8-bit grey-level optical potential landscapes with high fidelity and optical throughput via the GPC method. Three types of patterns were constructed: geometric block patterns, multi-level optical trap arrays, and optical obstacle arrays. Non-periodic patterns were accurately projected with an average of 80% diffraction efficiency. Periodic patterns yielded even higher diffraction efficiencies, averaging 94%, by the utilization of large-aperture phase contrast filters.
    Original languageEnglish
    Title of host publicationOptical trapping and optical micromanipulation 4
    EditorsK. Dholakia, G.C. Spalding
    PublisherInternational Society for Optical Engineering
    Publication date2007
    ISBN (Print)08-19-46792-8
    Publication statusPublished - 2007
    EventSPIE Optics and Photonics 2007 - San Diego, United States
    Duration: 26 Aug 200730 Aug 2007


    ConferenceSPIE Optics and Photonics 2007
    CountryUnited States
    CitySan Diego
    SeriesProceedings of SPIE, the International Society for Optical Engineering

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