Encapsulation and Survival of a Chondrocyte Cell Line within Xanthan Gum Derivative

Ana Carina Loureiro Mendes, Erkan T. Baran, Rui C. Pereira, Helena S. Azevedo, Rui L. Reis

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A chemical derivative of xanthan gum polysaccharide is investigated as a new artificial matrix for the encapsulation of chondrocytic cells. Toward this goal, a novel micro-droplet generator is developed to produce microcapsules. Microcapsules with an average diameter of 500 mu m, smooth surface, and homogeneous size distribution are obtained. ATDC5 cells encapsulated in carboxymethyl xanthan (CMX) microcapsules remain viable and are observed to proliferate for prolonged culture periods with enhanced metabolic activity. Furthermore, retention of the chondrogenic phenotype is exhibited by the cells within CMX, suggesting the ability of this material to be applied in cell-delivery therapies.
Original languageEnglish
JournalMacromolecular Bioscience
Volume12
Issue number3
Pages (from-to)350-359
Number of pages10
ISSN1616-5187
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • Cell culture
  • Cells
  • Polysaccharides
  • Encapsulation
  • carboxymethyl xanthan
  • polysaccharide
  • unclassified drug
  • xanthan
  • article
  • cartilage cell
  • cell encapsulation
  • cell proliferation
  • cell survival
  • cell viability
  • metabolism
  • microcapsule
  • phenotype
  • Animals
  • Capsules
  • Cell Line
  • Cell Proliferation
  • Cell Survival
  • Cells, Immobilized
  • Chondrocytes
  • Drug Carriers
  • Mice
  • Microscopy, Electron, Scanning
  • Particle Size
  • Polysaccharides, Bacterial
  • Average diameter
  • Carboxymethyl
  • Cell delivery
  • Chondrocyte cells
  • Chondrocytic
  • Chondrogenic phenotypes
  • Metabolic activity
  • Micro droplets
  • Micro-droplet
  • Microcapsules
  • Microencapsulation
  • Smooth surface
  • Xanthan gum
  • Microdroplet generator
  • 11138-66-2 xanthan gum
  • carboxymethyl xanthan microcapsule formulation
  • metabolic activity
  • xanthan gum polysaccharide derivative microcapsule
  • Rodentia Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals, Rodents, Vertebrates) - Muridae [86375] ATDC5 cell line cell_line murine chondrocyte cells
  • 02506, Cytology - Animal
  • 10511, Biophysics - Bioengineering
  • 18004, Bones, joints, fasciae, connective and adipose tissue - Physiology and biochemistry
  • Movement and Support
  • micro-droplet generator laboratory equipment
  • Biomaterials
  • Equipment Apparatus Devices and Instrumentation
  • Skeletal System
  • BIOCHEMISTRY
  • MATERIALS
  • POLYMER
  • HUMAN ARTICULAR CHONDROCYTES
  • MICROENCAPSULATION TECHNOLOGY
  • CHONDROGENIC DIFFERENTIATION
  • AQUEOUS-SOLUTION
  • GEL SYSTEM
  • CARTILAGE
  • HYDROGELS
  • REGENERATION
  • CHALLENGES
  • MEDICINE
  • cell delivery
  • microdroplet generator
  • microencapsulation
  • polysaccharides
  • xanthan gum

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