Enantioselective determination of methylphenidate and ritalinic acid in whole blood from forensic cases using automated solid-phase extraction and liquid chromatography-tandem mass spectrometry.

Ragnar Thomsen, Henrik B. Rasmussen, Kristian Linnet, Søren Brunak, Olivier Taboureau

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A chiral liquid chromatography tandem mass spectrometry (LC–MS-MS) method was developed and validated for quantifying methylphenidate and its major metabolite ritalinic acid in blood from
    forensic cases. Blood samples were prepared in a fully automated system by protein precipitation followed by solid-phase extraction. The LC–MS-MS method was linear in the range of 0.5 to 500 ng/g
    for the enantiomers of both analytes. For concentrations above the limit of quantification, coefficients of variation were 15% or less, and the accuracy was 89 to 94%. For 12 postmortem samples in
    which methylphenidate was not determined to be related to the cause of death, the femoral blood concentration of d-methylphenidate ranged from 5 to 58 ng/g, and from undetected to 48 ng/g for
    l-methylphenidate (median d/l-ratio 5.9). Ritalinic acid was present at concentrations 10–20 times higher with roughly equal amounts of the d- and l-forms. In blood from 10 living subjects that were not
    suspected of being intoxicated by methylphenidate, the concentration ranges and patterns were similar to those of the postmortem cases. Thus, methylphenidate does not appear to undergo significant
    postmortem redistribution.
    Original languageEnglish
    JournalJournal of Analytical Toxicology
    Volume36
    Pages (from-to)560-568
    ISSN0146-4760
    DOIs
    Publication statusPublished - 2012

    Bibliographical note

    DTU authors are members of the Indices Consortium

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