Yersinia ruckeri infection of rainbow trout: entrance portals and spread in the host

S. Deshmukh, U. Khimmakthong, J. K. Chettri, A. M. Bojesen, P. W. Kania, Inger Dalsgaard, K. Buchmann

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    Abstract

    The portal of entrance of either formalin inactivated or live Yersinia ruckeri organism in rainbow trout fish was studied by applying immunohistochemistry and in-situ hybridization. The sequential study involved a specific monoclonal antibody and a specific oligonucleotide probe binding to Yersinia ruckeri. It demonstrated the differential and regional uptake of both formalin inactivated and live bacterial organism in rainbow trout. The uptake dynamics in various organs/tissues demonstrated a site specific propensity between formalin inactivated and live bacteria. The possibility that lateral lines, dorsal fins and the gastro-intestinal tract could act as an active avenue to bacterial entrance was shown by both immunohistochemistry and in situ hybridization. The translocations of systemically absorbed formalin inactivated and live bacteria within different host body compartments were elucidated.
    Original languageEnglish
    Title of host publicationDAFINET Workshop and Ph.D. Course : Book of abstracts
    Number of pages1
    PublisherDAFINET
    Publication date2013
    Publication statusPublished - 2013
    EventDAFINET Workshop : Diagnosis and Control of Fish Diseases - Frederiksberg, Denmark
    Duration: 9 Apr 201311 Apr 2013
    http://www.dafinet.dk

    Conference

    ConferenceDAFINET Workshop : Diagnosis and Control of Fish Diseases
    CountryDenmark
    CityFrederiksberg
    Period09/04/201311/04/2013
    Internet address

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