TY - JOUR
T1 - Elevated numbers of SCART1+ γδ T cells in skin inflammation and inflammatory bowel disease
AU - Fink, Dorte Rosenbek
AU - Holm, Dorte
AU - Schlosser, Anders
AU - Nielsen, Ole
AU - Latta, Markus
AU - Lozano, Francisco
AU - Holmskov, Uffe
PY - 2010
Y1 - 2010
N2 - The members of the scavenger receptor cysteine-rich (SRCR) superfamily group B have diverse functions, including roles in the immune system. For years it has been known that the WC1 protein is expressed on the surface of bovine γδ T cells, and more recent studies indicate that WC1+ γδ T cells respond to stimulation with bacterial antigens by producing interferon-γ. The SRCR proteins CD5, CD6, Spα, CD163, and DMBT1/gp-340 are also involved in the immune response, since they are pattern recognition receptors capable of binding directly to bacterial and/or fungal components. Here, we investigate a novel murine SRCR protein named SCART1. The ectodomain and the full-length SCART1 were expressed in mammalian cells and used to raise monoclonal antibodies against the ectodomain for immunohistochemical and FACS analysis. Immunohistochemical analysis shows that SCART1 is expressed in a range of lymphoid organs and epithelial-rich tissues by a subset of T cells identified as being γδ T cells by FACS analysis. SCART1 was present in 86% of the γδ T cells and was not found in CD4+ or CD8+ T cells. The numbers of SCART1+ cells were elevated in two mouse models of human diseases: skin inflammation and inflammatory bowel disease. In the skin inflammation model, an 8.6-fold increase in SCART1+ cells was observed. Finally, recombinant SCART1 protein was found not to bind to selected bacterial or fungal components or to whole bacteria. Our results show that SCART1 is a novel γδ T cell marker and it is therefore likely that SCART1 plays a role in the immune response.
AB - The members of the scavenger receptor cysteine-rich (SRCR) superfamily group B have diverse functions, including roles in the immune system. For years it has been known that the WC1 protein is expressed on the surface of bovine γδ T cells, and more recent studies indicate that WC1+ γδ T cells respond to stimulation with bacterial antigens by producing interferon-γ. The SRCR proteins CD5, CD6, Spα, CD163, and DMBT1/gp-340 are also involved in the immune response, since they are pattern recognition receptors capable of binding directly to bacterial and/or fungal components. Here, we investigate a novel murine SRCR protein named SCART1. The ectodomain and the full-length SCART1 were expressed in mammalian cells and used to raise monoclonal antibodies against the ectodomain for immunohistochemical and FACS analysis. Immunohistochemical analysis shows that SCART1 is expressed in a range of lymphoid organs and epithelial-rich tissues by a subset of T cells identified as being γδ T cells by FACS analysis. SCART1 was present in 86% of the γδ T cells and was not found in CD4+ or CD8+ T cells. The numbers of SCART1+ cells were elevated in two mouse models of human diseases: skin inflammation and inflammatory bowel disease. In the skin inflammation model, an 8.6-fold increase in SCART1+ cells was observed. Finally, recombinant SCART1 protein was found not to bind to selected bacterial or fungal components or to whole bacteria. Our results show that SCART1 is a novel γδ T cell marker and it is therefore likely that SCART1 plays a role in the immune response.
U2 - 10.1016/j.molimm.2010.03.002
DO - 10.1016/j.molimm.2010.03.002
M3 - Journal article
C2 - 20381152
VL - 47
SP - 1710
EP - 1718
JO - Molecular Immunology
JF - Molecular Immunology
SN - 0161-5890
IS - 9
ER -