Electrospray mass spectrometry characterization of post-translational modifications of barley alpha-amylase 1 produced in yeast

M. Søgaard, J. S. Andersen, P. Roepsdorff, Birte Svensson

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Abstract

We have used electrospray mass spectrometry (ESMS) in combination with protein chemistry and genetics to delineate post-translational modifications in yeast of barley alpha-amylase 1 (AMY1), a 45 kD enzyme crucial for production of malt, an important starting material in the manufacture of beer and whisky. In addition to signal peptide processing these modifications are: (1) removal of C-terminal Arg-Ser by Kex1p, (2) glutathionylation of Cys95, (3) O-glycosylation, and (4) additional degradation of the C-terminus.
Original languageEnglish
JournalBIO-TECHNOLOGY
Volume11
Issue number10
Pages (from-to)1162-1165
ISSN0733-222X
Publication statusPublished - 1993
Externally publishedYes

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