Electrons selective uptake of a metal-reducing bacterium Shewanella oneidensis MR-1 from ferrocyanide

Zhiyong Zheng, Yong Xiao, Ranran Wu, Hans Erik Mølager Christensen, Feng Zhao, Jingdong Zhang*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The extracellular electron transfer of Shewanella oneidensis MR-1 (MR-1) has been extensively studied due to the importance of the biosensors and energy applications of bioelectrochemical systems. However, the oxidation of metal compounds by MR-1, which represents the inward extracellular electron transfer from extracellular electron donors into the microbe, is barely understood. In this study, MR-1 immobilized on an electrode electrocatalyzes the oxidation of [Fe(CN)6]4- to [Fe(CN)6]3- efficiently and selectively. The selectivity depends on midpoint potential and overall charge(s) of redox molecules. Among 12 investigated redox molecules, the negatively charged molecules with high midpoint potentials, i.e., [Ru(CN)6]4- and [Fe(CN)6]4-, show strong electrocatalysis. Neither reference bacteria (Escherichia coli K-12 nor Streptococcus mutans) electrocatalyze the oxidation of [Fe(CN)6]4-. The electrocatalysis decays when MR-1 is covered with palladium nanoparticles presumptively involved with cytochromes c. However, cytochromes c MtrC and OmcA on MR-1 do not play an essential role in this process. The results support a model that [Fe(CN)6]4- donor electrons to MR-1 by interacting with undiscovered active sites and the electrons are subsequently transferred to the electrode through the mediating effect of [Fe(CN)6]4-/3-. The selective electron uptake by MR-1 provides valuable insights into the fundamental insights of the applications of bioelectrochemical systems and the detection of specific redox molecules.
Original languageEnglish
Article number111571
JournalBiosensors and Bioelectronics
Volume142
Number of pages11
ISSN0956-5663
DOIs
Publication statusPublished - 2019

Keywords

  • Electrocatalysis
  • Shewanella
  • Bioelctrochemical systems
  • Extracellular electron transfer
  • Ferrocyanide
  • Cytochromes c

Cite this

Zheng, Zhiyong ; Xiao, Yong ; Wu, Ranran ; Christensen, Hans Erik Mølager ; Zhao, Feng ; Zhang, Jingdong. / Electrons selective uptake of a metal-reducing bacterium Shewanella oneidensis MR-1 from ferrocyanide. In: Biosensors and Bioelectronics. 2019 ; Vol. 142.
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title = "Electrons selective uptake of a metal-reducing bacterium Shewanella oneidensis MR-1 from ferrocyanide",
abstract = "The extracellular electron transfer of Shewanella oneidensis MR-1 (MR-1) has been extensively studied due to the importance of the biosensors and energy applications of bioelectrochemical systems. However, the oxidation of metal compounds by MR-1, which represents the inward extracellular electron transfer from extracellular electron donors into the microbe, is barely understood. In this study, MR-1 immobilized on an electrode electrocatalyzes the oxidation of [Fe(CN)6]4- to [Fe(CN)6]3- efficiently and selectively. The selectivity depends on midpoint potential and overall charge(s) of redox molecules. Among 12 investigated redox molecules, the negatively charged molecules with high midpoint potentials, i.e., [Ru(CN)6]4- and [Fe(CN)6]4-, show strong electrocatalysis. Neither reference bacteria (Escherichia coli K-12 nor Streptococcus mutans) electrocatalyze the oxidation of [Fe(CN)6]4-. The electrocatalysis decays when MR-1 is covered with palladium nanoparticles presumptively involved with cytochromes c. However, cytochromes c MtrC and OmcA on MR-1 do not play an essential role in this process. The results support a model that [Fe(CN)6]4- donor electrons to MR-1 by interacting with undiscovered active sites and the electrons are subsequently transferred to the electrode through the mediating effect of [Fe(CN)6]4-/3-. The selective electron uptake by MR-1 provides valuable insights into the fundamental insights of the applications of bioelectrochemical systems and the detection of specific redox molecules.",
keywords = "Electrocatalysis, Shewanella, Bioelctrochemical systems, Extracellular electron transfer, Ferrocyanide, Cytochromes c",
author = "Zhiyong Zheng and Yong Xiao and Ranran Wu and Christensen, {Hans Erik M{\o}lager} and Feng Zhao and Jingdong Zhang",
year = "2019",
doi = "10.1016/j.bios.2019.111571",
language = "English",
volume = "142",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier",

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Electrons selective uptake of a metal-reducing bacterium Shewanella oneidensis MR-1 from ferrocyanide. / Zheng, Zhiyong; Xiao, Yong; Wu, Ranran; Christensen, Hans Erik Mølager; Zhao, Feng; Zhang, Jingdong.

In: Biosensors and Bioelectronics, Vol. 142, 111571, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Electrons selective uptake of a metal-reducing bacterium Shewanella oneidensis MR-1 from ferrocyanide

AU - Zheng, Zhiyong

AU - Xiao, Yong

AU - Wu, Ranran

AU - Christensen, Hans Erik Mølager

AU - Zhao, Feng

AU - Zhang, Jingdong

PY - 2019

Y1 - 2019

N2 - The extracellular electron transfer of Shewanella oneidensis MR-1 (MR-1) has been extensively studied due to the importance of the biosensors and energy applications of bioelectrochemical systems. However, the oxidation of metal compounds by MR-1, which represents the inward extracellular electron transfer from extracellular electron donors into the microbe, is barely understood. In this study, MR-1 immobilized on an electrode electrocatalyzes the oxidation of [Fe(CN)6]4- to [Fe(CN)6]3- efficiently and selectively. The selectivity depends on midpoint potential and overall charge(s) of redox molecules. Among 12 investigated redox molecules, the negatively charged molecules with high midpoint potentials, i.e., [Ru(CN)6]4- and [Fe(CN)6]4-, show strong electrocatalysis. Neither reference bacteria (Escherichia coli K-12 nor Streptococcus mutans) electrocatalyze the oxidation of [Fe(CN)6]4-. The electrocatalysis decays when MR-1 is covered with palladium nanoparticles presumptively involved with cytochromes c. However, cytochromes c MtrC and OmcA on MR-1 do not play an essential role in this process. The results support a model that [Fe(CN)6]4- donor electrons to MR-1 by interacting with undiscovered active sites and the electrons are subsequently transferred to the electrode through the mediating effect of [Fe(CN)6]4-/3-. The selective electron uptake by MR-1 provides valuable insights into the fundamental insights of the applications of bioelectrochemical systems and the detection of specific redox molecules.

AB - The extracellular electron transfer of Shewanella oneidensis MR-1 (MR-1) has been extensively studied due to the importance of the biosensors and energy applications of bioelectrochemical systems. However, the oxidation of metal compounds by MR-1, which represents the inward extracellular electron transfer from extracellular electron donors into the microbe, is barely understood. In this study, MR-1 immobilized on an electrode electrocatalyzes the oxidation of [Fe(CN)6]4- to [Fe(CN)6]3- efficiently and selectively. The selectivity depends on midpoint potential and overall charge(s) of redox molecules. Among 12 investigated redox molecules, the negatively charged molecules with high midpoint potentials, i.e., [Ru(CN)6]4- and [Fe(CN)6]4-, show strong electrocatalysis. Neither reference bacteria (Escherichia coli K-12 nor Streptococcus mutans) electrocatalyze the oxidation of [Fe(CN)6]4-. The electrocatalysis decays when MR-1 is covered with palladium nanoparticles presumptively involved with cytochromes c. However, cytochromes c MtrC and OmcA on MR-1 do not play an essential role in this process. The results support a model that [Fe(CN)6]4- donor electrons to MR-1 by interacting with undiscovered active sites and the electrons are subsequently transferred to the electrode through the mediating effect of [Fe(CN)6]4-/3-. The selective electron uptake by MR-1 provides valuable insights into the fundamental insights of the applications of bioelectrochemical systems and the detection of specific redox molecules.

KW - Electrocatalysis

KW - Shewanella

KW - Bioelctrochemical systems

KW - Extracellular electron transfer

KW - Ferrocyanide

KW - Cytochromes c

U2 - 10.1016/j.bios.2019.111571

DO - 10.1016/j.bios.2019.111571

M3 - Journal article

VL - 142

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

M1 - 111571

ER -