Electrochemical sensing of biomarker for diagnostics of bacteria-specific infections

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Abstract

Aim: Pseudomonas aeruginosa is a pathogen that is prevalent in serious infections in compromised patients worldwide. A unique virulence factor of this bacterium is the redox-active molecule pyocyanin, which is a potential biomarker for the identification of P. aeruginosa infections. Here we report a direct, selective and rapid detection technique of pyocyanin. Materials & methods: Pyocyanin was detected by amperometry at a relatively high potential where the pyocyanin signal was unaffected by background contributions. Results & conclusion: Pyocyanin was detected at concentrations down to 125 nM in a 50 μM mixture of interfering compounds with a reproducibility of r2 = 0.999 (n = 5) within 200 s. The results document a step toward apoint-of-care technique for diagnosis of P. aeruginosa infections.
Original languageEnglish
JournalNanomedicine
Volume11
Issue number16
Pages (from-to)2185-2195
Number of pages11
ISSN1743-5889
DOIs
Publication statusPublished - 2016

Bibliographical note

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License.

Keywords

  • Amperometry
  • Bacteria
  • Biosensors
  • Cystic fibrosis
  • Detection
  • Diagnosis
  • Electrochemistry
  • Electrodes
  • Pseudomonas aeruginosa
  • Pyocyanin

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