Electrochemical sensing of biomarker for diagnostics of bacteria-specific infections

Fatima Al-Zahraa Al Atraktchi; Helle Krogh Johansen; Søren Molin; Winnie Edith Svendsen

doi:10.2217/nnm-2016-0155

Electrochemical sensing of biomarker for diagnostics of bacteria-specific infections

Fatima Al-Zahraa Al Atraktchi, Helle Krogh Johansen, Søren Molin, Winnie Edith Svendsen

Novo Nordisk Foundation Center for Biosustainability

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Abstract

Aim: Pseudomonas aeruginosa is a pathogen that is prevalent in serious infections in compromised patients worldwide. A unique virulence factor of this bacterium is the redox-active molecule pyocyanin, which is a potential biomarker for the identification of P. aeruginosa infections. Here we report a direct, selective and rapid detection technique of pyocyanin. Materials & methods: Pyocyanin was detected by amperometry at a relatively high potential where the pyocyanin signal was unaffected by background contributions. Results & conclusion: Pyocyanin was detected at concentrations down to 125 nM in a 50 μM mixture of interfering compounds with a reproducibility of r² = 0.999 (n = 5) within 200 s. The results document a step toward apoint-of-care technique for diagnosis of P. aeruginosa infections.

Original language	English
Journal	Nanomedicine
Volume	11
Issue number	16
Pages (from-to)	2185-2195
Number of pages	11
ISSN	1743-5889
DOIs	https://doi.org/10.2217/nnm-2016-0155
Publication status	Published - 2016

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License.

Keywords

Amperometry
Bacteria
Biosensors
Cystic fibrosis
Detection
Diagnosis
Electrochemistry
Electrodes
Pseudomonas aeruginosa
Pyocyanin

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title = "Electrochemical sensing of biomarker for diagnostics of bacteria-specific infections",

abstract = "Aim: Pseudomonas aeruginosa is a pathogen that is prevalent in serious infections in compromised patients worldwide. A unique virulence factor of this bacterium is the redox-active molecule pyocyanin, which is a potential biomarker for the identification of P. aeruginosa infections. Here we report a direct, selective and rapid detection technique of pyocyanin. Materials & methods: Pyocyanin was detected by amperometry at a relatively high potential where the pyocyanin signal was unaffected by background contributions. Results & conclusion: Pyocyanin was detected at concentrations down to 125 nM in a 50 μM mixture of interfering compounds with a reproducibility of r2 = 0.999 (n = 5) within 200 s. The results document a step toward apoint-of-care technique for diagnosis of P. aeruginosa infections.",

keywords = "Amperometry, Bacteria, Biosensors, Cystic fibrosis, Detection, Diagnosis, Electrochemistry, Electrodes, Pseudomonas aeruginosa, Pyocyanin",

author = "{Al Atraktchi}, {Fatima Al-Zahraa} and Johansen, {Helle Krogh} and S{\o}ren Molin and Svendsen, {Winnie Edith}",

note = "This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License.",

year = "2016",

doi = "10.2217/nnm-2016-0155",

language = "English",

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T1 - Electrochemical sensing of biomarker for diagnostics of bacteria-specific infections

AU - Al Atraktchi, Fatima Al-Zahraa

AU - Johansen, Helle Krogh

AU - Molin, Søren

AU - Svendsen, Winnie Edith

N1 - This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License.

PY - 2016

Y1 - 2016

N2 - Aim: Pseudomonas aeruginosa is a pathogen that is prevalent in serious infections in compromised patients worldwide. A unique virulence factor of this bacterium is the redox-active molecule pyocyanin, which is a potential biomarker for the identification of P. aeruginosa infections. Here we report a direct, selective and rapid detection technique of pyocyanin. Materials & methods: Pyocyanin was detected by amperometry at a relatively high potential where the pyocyanin signal was unaffected by background contributions. Results & conclusion: Pyocyanin was detected at concentrations down to 125 nM in a 50 μM mixture of interfering compounds with a reproducibility of r2 = 0.999 (n = 5) within 200 s. The results document a step toward apoint-of-care technique for diagnosis of P. aeruginosa infections.

AB - Aim: Pseudomonas aeruginosa is a pathogen that is prevalent in serious infections in compromised patients worldwide. A unique virulence factor of this bacterium is the redox-active molecule pyocyanin, which is a potential biomarker for the identification of P. aeruginosa infections. Here we report a direct, selective and rapid detection technique of pyocyanin. Materials & methods: Pyocyanin was detected by amperometry at a relatively high potential where the pyocyanin signal was unaffected by background contributions. Results & conclusion: Pyocyanin was detected at concentrations down to 125 nM in a 50 μM mixture of interfering compounds with a reproducibility of r2 = 0.999 (n = 5) within 200 s. The results document a step toward apoint-of-care technique for diagnosis of P. aeruginosa infections.

KW - Amperometry

KW - Bacteria

KW - Biosensors

KW - Cystic fibrosis

KW - Detection

KW - Diagnosis

KW - Electrochemistry

KW - Electrodes

KW - Pseudomonas aeruginosa

KW - Pyocyanin

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JO - Nanomedicine

JF - Nanomedicine

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ER -

Electrochemical sensing of biomarker for diagnostics of bacteria-specific infections

Abstract

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Keywords

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