Electrical Impedance Spectroscopy Microflow Cytometer for Cell Viability Tests

J.J. Gonzalez-Murillo, M. Monge-Azemar, J. Bartoli, A. Flores, M. Moreno, M. Garcia-Celma, A. Romano-Rodriguez, Winnie Edith Svendsen, J. Samitier, Romén Rodriguez-Trujíllo

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    Abstract

    The micro flow cytometer presented here is a combination of a set of coplanar electrodes and a microfluidic channel to test cell viability based on Single-cell Electrical Impedance Spectroscopy analysis. The device was tested both with a control and with a treated HeLa cells suspension. Impedance signals acquired were filtered and processed to obtain the data, classifying and clustering the results by dead and living cells to make the cell viability test. Results were compared with the standards of MTT and Neubauer Chamber viability tests, showing that 0.83μL of sample in the prototype designed and fabricated in this work was enough to obtain an approximate response of them.
    Original languageEnglish
    Title of host publication2018 Spanish Conference on Electron Devices (CDE)
    Number of pages4
    PublisherIEEE
    Publication date2018
    ISBN (Electronic)978-1-5386-5779-9
    DOIs
    Publication statusPublished - 2018
    Event12th Spanish Conference on Electron Devices - Hospedería del Colegio Fonseca, Salamanca, Spain
    Duration: 14 Nov 201816 Nov 2018
    Conference number: 12

    Conference

    Conference12th Spanish Conference on Electron Devices
    Number12
    LocationHospedería del Colegio Fonseca
    CountrySpain
    CitySalamanca
    Period14/11/201816/11/2018

    Cite this

    Gonzalez-Murillo, J. J., Monge-Azemar, M., Bartoli, J., Flores, A., Moreno, M., Garcia-Celma, M., ... Rodriguez-Trujíllo, R. (2018). Electrical Impedance Spectroscopy Microflow Cytometer for Cell Viability Tests. In 2018 Spanish Conference on Electron Devices (CDE) IEEE. https://doi.org/10.1109/CDE.2018.8596850
    Gonzalez-Murillo, J.J. ; Monge-Azemar, M. ; Bartoli, J. ; Flores, A. ; Moreno, M. ; Garcia-Celma, M. ; Romano-Rodriguez, A. ; Svendsen, Winnie Edith ; Samitier, J. ; Rodriguez-Trujíllo, Romén. / Electrical Impedance Spectroscopy Microflow Cytometer for Cell Viability Tests. 2018 Spanish Conference on Electron Devices (CDE). IEEE, 2018.
    @inproceedings{cf04cd79533044e084e76297b250d030,
    title = "Electrical Impedance Spectroscopy Microflow Cytometer for Cell Viability Tests",
    abstract = "The micro flow cytometer presented here is a combination of a set of coplanar electrodes and a microfluidic channel to test cell viability based on Single-cell Electrical Impedance Spectroscopy analysis. The device was tested both with a control and with a treated HeLa cells suspension. Impedance signals acquired were filtered and processed to obtain the data, classifying and clustering the results by dead and living cells to make the cell viability test. Results were compared with the standards of MTT and Neubauer Chamber viability tests, showing that 0.83μL of sample in the prototype designed and fabricated in this work was enough to obtain an approximate response of them.",
    author = "J.J. Gonzalez-Murillo and M. Monge-Azemar and J. Bartoli and A. Flores and M. Moreno and M. Garcia-Celma and A. Romano-Rodriguez and Svendsen, {Winnie Edith} and J. Samitier and Rom{\'e}n Rodriguez-Truj{\'i}llo",
    year = "2018",
    doi = "10.1109/CDE.2018.8596850",
    language = "English",
    booktitle = "2018 Spanish Conference on Electron Devices (CDE)",
    publisher = "IEEE",
    address = "United States",

    }

    Gonzalez-Murillo, JJ, Monge-Azemar, M, Bartoli, J, Flores, A, Moreno, M, Garcia-Celma, M, Romano-Rodriguez, A, Svendsen, WE, Samitier, J & Rodriguez-Trujíllo, R 2018, Electrical Impedance Spectroscopy Microflow Cytometer for Cell Viability Tests. in 2018 Spanish Conference on Electron Devices (CDE). IEEE, 12th Spanish Conference on Electron Devices, Salamanca, Spain, 14/11/2018. https://doi.org/10.1109/CDE.2018.8596850

    Electrical Impedance Spectroscopy Microflow Cytometer for Cell Viability Tests. / Gonzalez-Murillo, J.J.; Monge-Azemar, M.; Bartoli, J.; Flores, A.; Moreno, M.; Garcia-Celma, M.; Romano-Rodriguez, A.; Svendsen, Winnie Edith; Samitier, J.; Rodriguez-Trujíllo, Romén.

    2018 Spanish Conference on Electron Devices (CDE). IEEE, 2018.

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    TY - GEN

    T1 - Electrical Impedance Spectroscopy Microflow Cytometer for Cell Viability Tests

    AU - Gonzalez-Murillo, J.J.

    AU - Monge-Azemar, M.

    AU - Bartoli, J.

    AU - Flores, A.

    AU - Moreno, M.

    AU - Garcia-Celma, M.

    AU - Romano-Rodriguez, A.

    AU - Svendsen, Winnie Edith

    AU - Samitier, J.

    AU - Rodriguez-Trujíllo, Romén

    PY - 2018

    Y1 - 2018

    N2 - The micro flow cytometer presented here is a combination of a set of coplanar electrodes and a microfluidic channel to test cell viability based on Single-cell Electrical Impedance Spectroscopy analysis. The device was tested both with a control and with a treated HeLa cells suspension. Impedance signals acquired were filtered and processed to obtain the data, classifying and clustering the results by dead and living cells to make the cell viability test. Results were compared with the standards of MTT and Neubauer Chamber viability tests, showing that 0.83μL of sample in the prototype designed and fabricated in this work was enough to obtain an approximate response of them.

    AB - The micro flow cytometer presented here is a combination of a set of coplanar electrodes and a microfluidic channel to test cell viability based on Single-cell Electrical Impedance Spectroscopy analysis. The device was tested both with a control and with a treated HeLa cells suspension. Impedance signals acquired were filtered and processed to obtain the data, classifying and clustering the results by dead and living cells to make the cell viability test. Results were compared with the standards of MTT and Neubauer Chamber viability tests, showing that 0.83μL of sample in the prototype designed and fabricated in this work was enough to obtain an approximate response of them.

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    DO - 10.1109/CDE.2018.8596850

    M3 - Article in proceedings

    BT - 2018 Spanish Conference on Electron Devices (CDE)

    PB - IEEE

    ER -

    Gonzalez-Murillo JJ, Monge-Azemar M, Bartoli J, Flores A, Moreno M, Garcia-Celma M et al. Electrical Impedance Spectroscopy Microflow Cytometer for Cell Viability Tests. In 2018 Spanish Conference on Electron Devices (CDE). IEEE. 2018 https://doi.org/10.1109/CDE.2018.8596850