Bovine intestines and mesenteries in the European Union (EU) have to be removed from the food and feed chain. The opinion provides a quantitative assessment of the Bovine Spongiform Encephalopathy (BSE) infectious load that might enter the food and feed chain yearly if bovine intestine and mesentery from animals born and raised in the EU would be re-allowed for consumption. Data on the evolution of the BSE infectious titre; and of the weight of histological structures accumulating BSE infectivity, were collected. The Cattle TSE Monitoring Model (C-TSEMM) was used to estimate the number of BSE infected cattle entering undetected in the food and feed chain yearly. A model named TSEi was developed to estimates the BSE infectious load in tissues from infected animals at different ages and the total yearly infectious load that could enter the food and feed chain in the EU27. In BSE infected cattle, the infectivity associated with intestine and mesentery reaches its maximum in animals younger than 18 months and then progressively declines to a minimum value in animals older than 60 months. Due to the decline of the BSE prevalence in the EU, between 2007 and 2012, the yearly amount of BSE infectivity associated with intestine and mesentery (sent to destruction) from animals entering the food and feed chain was reduced by a factor of 10. However, over this period, the maximum level of exposure to the BSE agent for individuals that would have consumed these tissues remained stable. Finally, the TSEi model indicated that the removal of the last four metres of the small intestine and of the caecum from the food and feed chain would result in a major reduction of the BSE exposure risk associated with intestine and mesentery in cattle.