Efficient tumor cell lysis mediated by a Bcl-X(L) specific T cell clone isolated from a breast cancer patient

Rikke Bæk Sorensen, Sine Reker Hadrup, Tania Kollgaard, Inge Marie Svane, Per thor Straten, Mads Hald Andersen

Research output: Contribution to journalJournal articleResearchpeer-review


Based on the detection of spontaneous immune responses in cancer patients with cancer of different origin, Bcl-X(L) was recently described as a highly interesting tumor antigen recognized by CD8 positive cytotoxic T lymphocytes. To further characterize Bcl-X(L) as a tumor antigen we isolated and expanded Bcl-X(L) specific T cells from the peripheral blood of a breast cancer patient hosting a strong Bcl-X(L) specific T cell response. We describe that HLA-A2 restricted Bcl-X(L) specific T cell clones very efficiently lyse peptide pulsed T2 cells. Furthermore, tumor cell lines of different origin, i.e., breast cancer, colon cancer, and melanoma, are efficiently lysed in an HLA-dependent manner. Finally, ex vivo-isolated leukemia cells, but not non-malignant B and T cells are killed by Bcl-X(L) specific T cells. Our data underline Bcl-X(L) as an universal tumor antigen widely applicable in specific anticancer immunotherapy.
Original languageEnglish
JournalCancer Immunology, Immunotherapy
Issue number4
Pages (from-to)527-533
Number of pages7
Publication statusPublished - 2007
Externally publishedYes


  • Cancer Research
  • Immunology
  • Oncology
  • HLA A2 antigen
  • protein bcl x
  • protein bcl xl
  • tumor antigen
  • article
  • B lymphocyte
  • blood
  • breast cancer
  • cancer immunotherapy
  • cancer patient
  • cell clone
  • colon cancer
  • controlled study
  • cytolysis
  • ex vivo study
  • female
  • human
  • human cell
  • leukemia cell
  • melanoma
  • priority journal
  • T lymphocyte
  • tumor cell
  • tumor cell line
  • Antigens, Neoplasm
  • bcl-X Protein
  • Breast Neoplasms
  • Clone Cells
  • Cytotoxicity, Immunologic
  • Female
  • Flow Cytometry
  • HLA-A2 Antigen
  • Humans
  • Neoplasms
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocytes, Cytotoxic
  • BREAST cancer
  • BCL-X-L
  • efficient tumor cell lysis
  • T cell clone
  • breast cancer Breast Neoplasms (MeSH) neoplastic disease, reproductive system disease/female immunology
  • Primates Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Humans, Mammals, Primates, Vertebrates) - Hominidae [86215] human common female
  • Bcl-X(L)
  • HLA human leukocyte antigen
  • 02506, Cytology - Animal
  • 02508, Cytology - Human
  • 10064, Biochemistry studies - Proteins, peptides and amino acids
  • 15002, Blood - Blood and lymph studies
  • 15004, Blood - Blood cell studies
  • 16504, Reproductive system - Physiology and biochemistry
  • 16506, Reproductive system - Pathology
  • 24003, Neoplasms - Immunology
  • 24004, Neoplasms - Pathology, clinical aspects and systemic effects
  • 34502, Immunology - General and methods
  • 34508, Immunology - Immunopathology, tissue immunology
  • Human Medicine, Medical Sciences
  • breast reproductive system
  • peripheral blood blood and lymphatics
  • T cell immune system, blood and lymphatics
  • Clinical Immunology
  • Gynecology


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