Abstract
Based on the detection of spontaneous immune responses in cancer patients with cancer of different origin, Bcl-X(L) was recently described as a highly interesting tumor antigen recognized by CD8 positive cytotoxic T lymphocytes. To further characterize Bcl-X(L) as a tumor antigen we isolated and expanded Bcl-X(L) specific T cells from the peripheral blood of a breast cancer patient hosting a strong Bcl-X(L) specific T cell response. We describe that HLA-A2 restricted Bcl-X(L) specific T cell clones very efficiently lyse peptide pulsed T2 cells. Furthermore, tumor cell lines of different origin, i.e., breast cancer, colon cancer, and melanoma, are efficiently lysed in an HLA-dependent manner. Finally, ex vivo-isolated leukemia cells, but not non-malignant B and T cells are killed by Bcl-X(L) specific T cells. Our data underline Bcl-X(L) as an universal tumor antigen widely applicable in specific anticancer immunotherapy.
| Original language | English |
|---|---|
| Journal | Cancer Immunology, Immunotherapy |
| Volume | 56 |
| Issue number | 4 |
| Pages (from-to) | 527-533 |
| Number of pages | 7 |
| ISSN | 0340-7004 |
| DOIs | |
| Publication status | Published - 2007 |
| Externally published | Yes |
Keywords
- Cancer Research
- Immunology
- Oncology
- HLA A2 antigen
- protein bcl x
- protein bcl xl
- tumor antigen
- article
- B lymphocyte
- blood
- breast cancer
- cancer immunotherapy
- cancer patient
- cell clone
- colon cancer
- controlled study
- cytolysis
- ex vivo study
- female
- human
- human cell
- leukemia cell
- melanoma
- priority journal
- T lymphocyte
- tumor cell
- tumor cell line
- Antigens, Neoplasm
- bcl-X Protein
- Breast Neoplasms
- Clone Cells
- Cytotoxicity, Immunologic
- Female
- Flow Cytometry
- HLA-A2 Antigen
- Humans
- Neoplasms
- Reverse Transcriptase Polymerase Chain Reaction
- T-Lymphocytes, Cytotoxic
- BREAST cancer
- ONCOLOGY
- IMMUNOLOGY
- BCL-X-L
- SURVIVIN
- APOPTOSIS
- REGULATOR
- DEATH
- efficient tumor cell lysis
- T cell clone
- breast cancer Breast Neoplasms (MeSH) neoplastic disease, reproductive system disease/female immunology
- Primates Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Humans, Mammals, Primates, Vertebrates) - Hominidae [86215] human common female
- Bcl-X(L)
- HLA human leukocyte antigen
- 02506, Cytology - Animal
- 02508, Cytology - Human
- 10064, Biochemistry studies - Proteins, peptides and amino acids
- 15002, Blood - Blood and lymph studies
- 15004, Blood - Blood cell studies
- 16504, Reproductive system - Physiology and biochemistry
- 16506, Reproductive system - Pathology
- 24003, Neoplasms - Immunology
- 24004, Neoplasms - Pathology, clinical aspects and systemic effects
- 34502, Immunology - General and methods
- 34508, Immunology - Immunopathology, tissue immunology
- Human Medicine, Medical Sciences
- breast reproductive system
- peripheral blood blood and lymphatics
- T cell immune system, blood and lymphatics
- Clinical Immunology
- Gynecology
