Efficient reverse transcription using locked nucleic acid nucleotides towards the evolution of nuclease resistant RNA aptamers.

Lucile Crouzier, Camille Dubois, Stacey L Edwards, Lasse H Lauridsen, Jesper Wengel, Rakesh N. Veedu

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Abstract

We found that SuperScript® III Reverse Transcriptase is an efficient enzyme for the recognition of LNA nucleotides, making it a prime candidate to be used in de novo selection of LNA containing RNA aptamers.
Original languageEnglish
JournalP L o S One
Volume7
Issue number4
Pages (from-to)e35990
ISSN1932-6203
Publication statusPublished - 2012

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