Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration

Research output: Contribution to journalJournal article – Annual report year: 2018Researchpeer-review

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Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration. / Tajsoleiman, Tannaz; Jafar Abdekhodaie, Mohammad; Gernaey, Krist V.; Krühne, Ulrich.

In: Bioengineering, Vol. 5, No. 2, 33, 2018.

Research output: Contribution to journalJournal article – Annual report year: 2018Researchpeer-review

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@article{a94faae8c1424517af1a6939376f4132,
title = "Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration",
abstract = "Due to the sensitivity of mammalian cell cultures, understanding the influence of operating conditions during a tissue generation procedure is crucial. In this regard, a detailed study of scaffold based cell culture under a perfusion flow is presented with the aid of mathematical modelling and computational fluid dynamics (CFD). With respect to the complexity of the case study, this work focuses solely on the effect of nutrient and metabolite concentrations, and the possible influence of fluid-induced shear stress on a targeted cell (cartilage) culture. The simulation set up gives the possibility of predicting the cell culture behavior under various operating conditions and scaffold designs. Thereby, the exploitation of the predictive simulation into a newly developed stochastic routine provides the opportunity of exploring improved scaffold geometry designs. This approach was applied on a common type of fibrous structure in order to increase the process efficiencies compared with the regular used formats. The suggested topology supplies a larger effective surface for cell attachment compared to the reference design while the level of shear stress is kept at the positive range of effect. Moreover, significant improvement of mass transfer is predicted for the suggested topology.",
keywords = "Tissue engineering, CFD simulation, Scaffold geometry optimization, Micro-bioreactor, Operating conditions",
author = "Tannaz Tajsoleiman and {Jafar Abdekhodaie}, Mohammad and Gernaey, {Krist V.} and Ulrich Kr{\"u}hne",
year = "2018",
doi = "10.3390/bioengineering5020033",
language = "English",
volume = "5",
journal = "Bioengineering",
issn = "2306-5354",
publisher = "M D P I AG",
number = "2",

}

RIS

TY - JOUR

T1 - Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration

AU - Tajsoleiman, Tannaz

AU - Jafar Abdekhodaie, Mohammad

AU - Gernaey, Krist V.

AU - Krühne, Ulrich

PY - 2018

Y1 - 2018

N2 - Due to the sensitivity of mammalian cell cultures, understanding the influence of operating conditions during a tissue generation procedure is crucial. In this regard, a detailed study of scaffold based cell culture under a perfusion flow is presented with the aid of mathematical modelling and computational fluid dynamics (CFD). With respect to the complexity of the case study, this work focuses solely on the effect of nutrient and metabolite concentrations, and the possible influence of fluid-induced shear stress on a targeted cell (cartilage) culture. The simulation set up gives the possibility of predicting the cell culture behavior under various operating conditions and scaffold designs. Thereby, the exploitation of the predictive simulation into a newly developed stochastic routine provides the opportunity of exploring improved scaffold geometry designs. This approach was applied on a common type of fibrous structure in order to increase the process efficiencies compared with the regular used formats. The suggested topology supplies a larger effective surface for cell attachment compared to the reference design while the level of shear stress is kept at the positive range of effect. Moreover, significant improvement of mass transfer is predicted for the suggested topology.

AB - Due to the sensitivity of mammalian cell cultures, understanding the influence of operating conditions during a tissue generation procedure is crucial. In this regard, a detailed study of scaffold based cell culture under a perfusion flow is presented with the aid of mathematical modelling and computational fluid dynamics (CFD). With respect to the complexity of the case study, this work focuses solely on the effect of nutrient and metabolite concentrations, and the possible influence of fluid-induced shear stress on a targeted cell (cartilage) culture. The simulation set up gives the possibility of predicting the cell culture behavior under various operating conditions and scaffold designs. Thereby, the exploitation of the predictive simulation into a newly developed stochastic routine provides the opportunity of exploring improved scaffold geometry designs. This approach was applied on a common type of fibrous structure in order to increase the process efficiencies compared with the regular used formats. The suggested topology supplies a larger effective surface for cell attachment compared to the reference design while the level of shear stress is kept at the positive range of effect. Moreover, significant improvement of mass transfer is predicted for the suggested topology.

KW - Tissue engineering

KW - CFD simulation

KW - Scaffold geometry optimization

KW - Micro-bioreactor

KW - Operating conditions

U2 - 10.3390/bioengineering5020033

DO - 10.3390/bioengineering5020033

M3 - Journal article

VL - 5

JO - Bioengineering

JF - Bioengineering

SN - 2306-5354

IS - 2

M1 - 33

ER -