Effects of temperature, ultraviolet radiation and pectin methyl esterase on aerobic methane release from plant material

Dan Bruhn; Teis Nørgaard Mikkelsen; J. Øbro; W.G.T. Willats; Per Ambus

doi:10.1111/j.1438-8677.2009.00202.x

Effects of temperature, ultraviolet radiation and pectin methyl esterase on aerobic methane release from plant material

Dan Bruhn, Teis Nørgaard Mikkelsen, J. Øbro, W.G.T. Willats, Per Ambus

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

This study examines the effects of different irradiance types on aerobic methane (CH4) efflux rates from terrestrial plant material. Furthermore, the role of the enzyme pectin methyl esterase (PME) on CH4 efflux potential was also examined. Different types of plant tissue and purified pectin were incubated in glass vials with different combinations of irradiation and/or temperature. Purified dry pectin was incubated in solution, and with or without PME. Before and after incubation, the concentration of CH4 was measured with a gas chromatograph. Rates of CH4 emission were found to depend exponentially on temperature and linearly on UV-B irradiance. UV-B had a greater stimulating effect than UV-A, while visible light had no effect on emission rates. PME was found to substantially reduce the potential for aerobic CH4 emissions upon demethylation of pectin.

Original language	English
Journal	Plant Biology
Volume	11
Issue number	Suppl.1
Pages (from-to)	43-48
ISSN	1435-8603
DOIs	https://doi.org/10.1111/j.1438-8677.2009.00202.x
Publication status	Published - 2009

Keywords

Bio energy
Ecosystems, climate effects, greenhouse gasses

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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10.1111/j.1438-8677.2009.00202.x

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title = "Effects of temperature, ultraviolet radiation and pectin methyl esterase on aerobic methane release from plant material",

abstract = "This study examines the effects of different irradiance types on aerobic methane (CH4) efflux rates from terrestrial plant material. Furthermore, the role of the enzyme pectin methyl esterase (PME) on CH4 efflux potential was also examined. Different types of plant tissue and purified pectin were incubated in glass vials with different combinations of irradiation and/or temperature. Purified dry pectin was incubated in solution, and with or without PME. Before and after incubation, the concentration of CH4 was measured with a gas chromatograph. Rates of CH4 emission were found to depend exponentially on temperature and linearly on UV-B irradiance. UV-B had a greater stimulating effect than UV-A, while visible light had no effect on emission rates. PME was found to substantially reduce the potential for aerobic CH4 emissions upon demethylation of pectin.",

keywords = "Bio energy, Ecosystems, climate effects, greenhouse gasses, Bioenergi, {\O}kosystemer, klimaeffekter, drivhusgasser",

author = "Dan Bruhn and Mikkelsen, {Teis N{\o}rgaard} and J. {\O}bro and W.G.T. Willats and Per Ambus",

year = "2009",

doi = "10.1111/j.1438-8677.2009.00202.x",

language = "English",

volume = "11",

pages = "43--48",

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T1 - Effects of temperature, ultraviolet radiation and pectin methyl esterase on aerobic methane release from plant material

AU - Bruhn, Dan

AU - Mikkelsen, Teis Nørgaard

AU - Øbro, J.

AU - Willats, W.G.T.

AU - Ambus, Per

PY - 2009

Y1 - 2009

N2 - This study examines the effects of different irradiance types on aerobic methane (CH4) efflux rates from terrestrial plant material. Furthermore, the role of the enzyme pectin methyl esterase (PME) on CH4 efflux potential was also examined. Different types of plant tissue and purified pectin were incubated in glass vials with different combinations of irradiation and/or temperature. Purified dry pectin was incubated in solution, and with or without PME. Before and after incubation, the concentration of CH4 was measured with a gas chromatograph. Rates of CH4 emission were found to depend exponentially on temperature and linearly on UV-B irradiance. UV-B had a greater stimulating effect than UV-A, while visible light had no effect on emission rates. PME was found to substantially reduce the potential for aerobic CH4 emissions upon demethylation of pectin.

AB - This study examines the effects of different irradiance types on aerobic methane (CH4) efflux rates from terrestrial plant material. Furthermore, the role of the enzyme pectin methyl esterase (PME) on CH4 efflux potential was also examined. Different types of plant tissue and purified pectin were incubated in glass vials with different combinations of irradiation and/or temperature. Purified dry pectin was incubated in solution, and with or without PME. Before and after incubation, the concentration of CH4 was measured with a gas chromatograph. Rates of CH4 emission were found to depend exponentially on temperature and linearly on UV-B irradiance. UV-B had a greater stimulating effect than UV-A, while visible light had no effect on emission rates. PME was found to substantially reduce the potential for aerobic CH4 emissions upon demethylation of pectin.

KW - Bio energy

KW - Ecosystems, climate effects, greenhouse gasses

KW - Bioenergi

KW - Økosystemer, klimaeffekter, drivhusgasser

U2 - 10.1111/j.1438-8677.2009.00202.x

DO - 10.1111/j.1438-8677.2009.00202.x

M3 - Journal article

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VL - 11

SP - 43

EP - 48

JO - Plant Biology

JF - Plant Biology

SN - 1435-8603

IS - Suppl.1

ER -