Effects of specific carbohydrates on the intestinal microbiota

Lene Hemmingsen, Jesper Holck, Anne S. Meyer, Andrea Wilcks, Tine Rask Licht

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review


The current screening study aimed at testing a set of well-characterized carbohydrates derived from pectic oligosaccharides (POS) from sugar beet for their specific effect on intestinal microbiotas derived from healthy people and from patients suffering from the inflammatory bowel disease designated Ulcerative Colitis (UC). Two such oligosaccharides having different degrees of polymerization, in the following designated S1 and S2, respectively, were tested. Small scale anaerobic fermentation studies were performed to test the effect of S1 and S2 on the composition of the intestinal microbiotas. Changes in the microbial composition were addressed by Denaturing Gradient Gel Electrophoresis, DGGE, using Fructo- Oligosaccharides (FOS, a goldenstandard prebiotic) and glucose as reference substrates. Comparison between the DGGE profiles obtained by fermentations of S1, S2 and FOS showed that S2 produced a DGGE profile different from fermentations of S1 and the control substrate FOS in a Pearson correlation cluster analysis, indicating that the degree of polymerization (DP) was decisive for which bacteria were stimulated by the oligosaccharides. Additionally, DGGE results of this screening study showed that there were no significant differences between the numbers of bands in the fermentations of all four substrates, indicating that S1, S2 and FOS had similar degrees of selectivity.
Original languageEnglish
Publication date2010
Publication statusPublished - 2010
Event2nd TNO Beneficial Microbes Conference 2010 - NH Conference Centre Leeuwenhorst, Noordwijkerhout, Netherlands
Duration: 15 Mar 201017 Mar 2010
Conference number: 2


Conference2nd TNO Beneficial Microbes Conference 2010
LocationNH Conference Centre Leeuwenhorst
Internet address


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