The current screening study aimed at testing a set of well-characterized carbohydrates derived from pectic oligosaccharides (POS) from sugar beet for their specific effect on intestinal microbiotas derived from healthy people and from patients suffering from the inflammatory bowel disease designated Ulcerative Colitis (UC). Two such oligosaccharides having different degrees of polymerization, in the following designated S1 and S2, respectively, were tested. Small scale anaerobic fermentation studies were performed to test the effect of S1 and S2 on the composition of the intestinal microbiotas. Changes in the microbial composition were addressed by Denaturing Gradient Gel Electrophoresis, DGGE, using Fructo- Oligosaccharides (FOS, a goldenstandard prebiotic) and glucose as reference substrates. Comparison between the DGGE profiles obtained by fermentations of S1, S2 and FOS showed that S2 produced a DGGE profile different from fermentations of S1 and the control substrate FOS in a Pearson correlation cluster analysis, indicating that the degree of polymerization (DP) was decisive for which bacteria were stimulated by the oligosaccharides. Additionally, DGGE results of this screening study showed that there were no significant differences between the numbers of bands in the fermentations of all four substrates, indicating that S1, S2 and FOS had similar degrees of selectivity.
|Publication status||Published - 2010|
|Event||2nd TNO Beneficial Microbes Conference 2010 - NH Conference Centre Leeuwenhorst, Noordwijkerhout, Netherlands|
Duration: 15 Mar 2010 → 17 Mar 2010
Conference number: 2
|Conference||2nd TNO Beneficial Microbes Conference 2010|
|Location||NH Conference Centre Leeuwenhorst|
|Period||15/03/2010 → 17/03/2010|