Effects of soluble immunostimulants on mucosal immune responses in rainbow trout immersion-vaccinated against Yersinia ruckeri

Jakob Skov, Jiwan Kumar Chettri, Rzgar M. Jaafar, Per W. Kania, Inger Dalsgaard, Kurt Buchmann*

*Corresponding author for this work

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Immersion vaccination of rainbow trout against Yersinia ruckeri infection is an established method to prevent enteric red mouth disease (ERM) but the effect is inferior to injection vaccination and the duration of protection is limited to less than six months. Adjuvants in vaccines may in general elevate the immune response and the present work elucidates how ERM immersion vaccination of trout in combination with exposure to soluble adjuvants, Montanide™ IMS 1312 VG PR and β-glucan, affects immune reactions. The former adjuvant, when used alone, induced a slightly increased protection (not statistically significant) whereas β-glucan did not increase protection. Adjuvant-treated and non-exposed groups showed elevated plasma lysozyme activity after challenge with Y. ruckeri. Specific antibody production was not positively affected by combining adjuvant and vaccine. Overall expression of immune genes tested was generally manifold higher in gills compared to skin. Only genes encoding SAA and IL-17C1 were expressed at a higher level in skin. Dynamic differences between the gill and skin compartments were also recorded for genes encoding cytokines (TNF-α, IL-1β, IL-6, IL-10, IL-12, IL-17A/F2, IL-17C1, IL-17C2, IL-22), immunoglobulins (IgM, IgD, IgT), cell markers (CD8α, TCR-β) and acute phase reactants (SAA, lysozyme). These genes were upregulated 24 h post-vaccination in fish gills exposed to both vaccine-adjuvant combinations when compared to fish exposed to vaccine alone. After a few weeks no vaccine induced reaction was seen and after challenge with bacteria mainly unvaccinated fish responded. Adjuvants used in combination with immersion vaccine clearly influences immune reactions and may improve duration and protection but further potency tests should be performed.
    Original languageEnglish
    JournalAquaculture
    Volume492
    Pages (from-to)237-246
    ISSN0044-8486
    DOIs
    Publication statusPublished - 2018

    Keywords

    • Yersinia ruckeri
    • Immersion vaccination
    • Adjuvant
    • Montanide™
    • β-glucan

    Cite this

    Skov, Jakob ; Chettri, Jiwan Kumar ; Jaafar, Rzgar M. ; Kania, Per W. ; Dalsgaard, Inger ; Buchmann, Kurt. / Effects of soluble immunostimulants on mucosal immune responses in rainbow trout immersion-vaccinated against Yersinia ruckeri. In: Aquaculture. 2018 ; Vol. 492. pp. 237-246.
    @article{8b04cad9129c4cfdbbf37fd648e086f9,
    title = "Effects of soluble immunostimulants on mucosal immune responses in rainbow trout immersion-vaccinated against Yersinia ruckeri",
    abstract = "Immersion vaccination of rainbow trout against Yersinia ruckeri infection is an established method to prevent enteric red mouth disease (ERM) but the effect is inferior to injection vaccination and the duration of protection is limited to less than six months. Adjuvants in vaccines may in general elevate the immune response and the present work elucidates how ERM immersion vaccination of trout in combination with exposure to soluble adjuvants, Montanide™ IMS 1312 VG PR and β-glucan, affects immune reactions. The former adjuvant, when used alone, induced a slightly increased protection (not statistically significant) whereas β-glucan did not increase protection. Adjuvant-treated and non-exposed groups showed elevated plasma lysozyme activity after challenge with Y. ruckeri. Specific antibody production was not positively affected by combining adjuvant and vaccine. Overall expression of immune genes tested was generally manifold higher in gills compared to skin. Only genes encoding SAA and IL-17C1 were expressed at a higher level in skin. Dynamic differences between the gill and skin compartments were also recorded for genes encoding cytokines (TNF-α, IL-1β, IL-6, IL-10, IL-12, IL-17A/F2, IL-17C1, IL-17C2, IL-22), immunoglobulins (IgM, IgD, IgT), cell markers (CD8α, TCR-β) and acute phase reactants (SAA, lysozyme). These genes were upregulated 24 h post-vaccination in fish gills exposed to both vaccine-adjuvant combinations when compared to fish exposed to vaccine alone. After a few weeks no vaccine induced reaction was seen and after challenge with bacteria mainly unvaccinated fish responded. Adjuvants used in combination with immersion vaccine clearly influences immune reactions and may improve duration and protection but further potency tests should be performed.",
    keywords = "Yersinia ruckeri , Immersion vaccination , Adjuvant , Montanide™ , β-glucan",
    author = "Jakob Skov and Chettri, {Jiwan Kumar} and Jaafar, {Rzgar M.} and Kania, {Per W.} and Inger Dalsgaard and Kurt Buchmann",
    year = "2018",
    doi = "10.1016/j.aquaculture.2018.04.011",
    language = "English",
    volume = "492",
    pages = "237--246",
    journal = "Aquaculture",
    issn = "0044-8486",
    publisher = "Elsevier",

    }

    Effects of soluble immunostimulants on mucosal immune responses in rainbow trout immersion-vaccinated against Yersinia ruckeri. / Skov, Jakob; Chettri, Jiwan Kumar; Jaafar, Rzgar M.; Kania, Per W.; Dalsgaard, Inger; Buchmann, Kurt.

    In: Aquaculture, Vol. 492, 2018, p. 237-246.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Effects of soluble immunostimulants on mucosal immune responses in rainbow trout immersion-vaccinated against Yersinia ruckeri

    AU - Skov, Jakob

    AU - Chettri, Jiwan Kumar

    AU - Jaafar, Rzgar M.

    AU - Kania, Per W.

    AU - Dalsgaard, Inger

    AU - Buchmann, Kurt

    PY - 2018

    Y1 - 2018

    N2 - Immersion vaccination of rainbow trout against Yersinia ruckeri infection is an established method to prevent enteric red mouth disease (ERM) but the effect is inferior to injection vaccination and the duration of protection is limited to less than six months. Adjuvants in vaccines may in general elevate the immune response and the present work elucidates how ERM immersion vaccination of trout in combination with exposure to soluble adjuvants, Montanide™ IMS 1312 VG PR and β-glucan, affects immune reactions. The former adjuvant, when used alone, induced a slightly increased protection (not statistically significant) whereas β-glucan did not increase protection. Adjuvant-treated and non-exposed groups showed elevated plasma lysozyme activity after challenge with Y. ruckeri. Specific antibody production was not positively affected by combining adjuvant and vaccine. Overall expression of immune genes tested was generally manifold higher in gills compared to skin. Only genes encoding SAA and IL-17C1 were expressed at a higher level in skin. Dynamic differences between the gill and skin compartments were also recorded for genes encoding cytokines (TNF-α, IL-1β, IL-6, IL-10, IL-12, IL-17A/F2, IL-17C1, IL-17C2, IL-22), immunoglobulins (IgM, IgD, IgT), cell markers (CD8α, TCR-β) and acute phase reactants (SAA, lysozyme). These genes were upregulated 24 h post-vaccination in fish gills exposed to both vaccine-adjuvant combinations when compared to fish exposed to vaccine alone. After a few weeks no vaccine induced reaction was seen and after challenge with bacteria mainly unvaccinated fish responded. Adjuvants used in combination with immersion vaccine clearly influences immune reactions and may improve duration and protection but further potency tests should be performed.

    AB - Immersion vaccination of rainbow trout against Yersinia ruckeri infection is an established method to prevent enteric red mouth disease (ERM) but the effect is inferior to injection vaccination and the duration of protection is limited to less than six months. Adjuvants in vaccines may in general elevate the immune response and the present work elucidates how ERM immersion vaccination of trout in combination with exposure to soluble adjuvants, Montanide™ IMS 1312 VG PR and β-glucan, affects immune reactions. The former adjuvant, when used alone, induced a slightly increased protection (not statistically significant) whereas β-glucan did not increase protection. Adjuvant-treated and non-exposed groups showed elevated plasma lysozyme activity after challenge with Y. ruckeri. Specific antibody production was not positively affected by combining adjuvant and vaccine. Overall expression of immune genes tested was generally manifold higher in gills compared to skin. Only genes encoding SAA and IL-17C1 were expressed at a higher level in skin. Dynamic differences between the gill and skin compartments were also recorded for genes encoding cytokines (TNF-α, IL-1β, IL-6, IL-10, IL-12, IL-17A/F2, IL-17C1, IL-17C2, IL-22), immunoglobulins (IgM, IgD, IgT), cell markers (CD8α, TCR-β) and acute phase reactants (SAA, lysozyme). These genes were upregulated 24 h post-vaccination in fish gills exposed to both vaccine-adjuvant combinations when compared to fish exposed to vaccine alone. After a few weeks no vaccine induced reaction was seen and after challenge with bacteria mainly unvaccinated fish responded. Adjuvants used in combination with immersion vaccine clearly influences immune reactions and may improve duration and protection but further potency tests should be performed.

    KW - Yersinia ruckeri

    KW - Immersion vaccination

    KW - Adjuvant

    KW - Montanide™

    KW - β-glucan

    U2 - 10.1016/j.aquaculture.2018.04.011

    DO - 10.1016/j.aquaculture.2018.04.011

    M3 - Journal article

    VL - 492

    SP - 237

    EP - 246

    JO - Aquaculture

    JF - Aquaculture

    SN - 0044-8486

    ER -