TY - JOUR
T1 - Effects of pristine and photoaged tire wear particles and their leachable additives on key nitrogen removal processes and nitrous oxide accumulation in estuarine sediments
AU - Ye, Jinyu
AU - Gao, Yuan
AU - Gao, Huan
AU - Zhao, Qingqing
AU - Xu, Dan
AU - Zhou, Minjie
AU - Shi, Meng
AU - Xue, Xiangdong
PY - 2025
Y1 - 2025
N2 - Despite growing attention to the environmental pollution caused by tire
wear particles (TWPs), the effects of pristine and photoaged TWPs
(P-TWPs and A-TWPs) and their TWP leachates (TWPLs; P-TWPL and A-TWPL)
on key nitrogen removal processes in estuarine sediments remain unclear.
This study explores the responses of the denitrification rate, anammox
rate, and nitrous oxide (N2O) accumulation to P-TWP, A-TWP,
P-TWPL, and A-TWPL exposure in estuarine sediments, and assesses the
potential biotoxic substances present in TWPLs. P-TWPs reduced the
denitrification rate by 17.1 ± 10.0 % and increased N2O accumulation by 28.1 ± 18.7 %. The A-TWPs not only reduced the denitrification rate by 31.3 ± 8.3 % and increased N2O
accumulation by 43.1 ± 22.0 %, but also decreased the anammox rate by
22.1 ± 13.3 %. A-TWPs further inhibited the denitrification rate by
reducing nitrate reductase activity and the abundance of its gene (narG ), while simultaneously decreasing hydrazine synthase activity and the abundance of its gene (hzo), thereby slowing the anammox rate. N2O accumulation after exposure to TWPs and TWPLs was positively correlated with the activity ratio of N2O-producing and N2O-consuming
enzymes. Zinc (Zn) release in A-TWPL was 48.5 ± 6.9 % higher than that
in P-TWPL, which is a crucial reason for the higher biotoxicity produced
by A-TWPs. In addition, the abundance of denitrifying and anammox
bacteria closely linked to the Zn, manganese, and arsenic concentrations
in the TWPLs. This study provides insights into assessing the
environmental risks posed by TWPs to estuarine ecosystems.
AB - Despite growing attention to the environmental pollution caused by tire
wear particles (TWPs), the effects of pristine and photoaged TWPs
(P-TWPs and A-TWPs) and their TWP leachates (TWPLs; P-TWPL and A-TWPL)
on key nitrogen removal processes in estuarine sediments remain unclear.
This study explores the responses of the denitrification rate, anammox
rate, and nitrous oxide (N2O) accumulation to P-TWP, A-TWP,
P-TWPL, and A-TWPL exposure in estuarine sediments, and assesses the
potential biotoxic substances present in TWPLs. P-TWPs reduced the
denitrification rate by 17.1 ± 10.0 % and increased N2O accumulation by 28.1 ± 18.7 %. The A-TWPs not only reduced the denitrification rate by 31.3 ± 8.3 % and increased N2O
accumulation by 43.1 ± 22.0 %, but also decreased the anammox rate by
22.1 ± 13.3 %. A-TWPs further inhibited the denitrification rate by
reducing nitrate reductase activity and the abundance of its gene (narG ), while simultaneously decreasing hydrazine synthase activity and the abundance of its gene (hzo), thereby slowing the anammox rate. N2O accumulation after exposure to TWPs and TWPLs was positively correlated with the activity ratio of N2O-producing and N2O-consuming
enzymes. Zinc (Zn) release in A-TWPL was 48.5 ± 6.9 % higher than that
in P-TWPL, which is a crucial reason for the higher biotoxicity produced
by A-TWPs. In addition, the abundance of denitrifying and anammox
bacteria closely linked to the Zn, manganese, and arsenic concentrations
in the TWPLs. This study provides insights into assessing the
environmental risks posed by TWPs to estuarine ecosystems.
KW - Anammox
KW - Denitrification
KW - Leachable additives
KW - Nitrous oxide
KW - Tire wear particles
U2 - 10.1016/j.jhazmat.2025.137136
DO - 10.1016/j.jhazmat.2025.137136
M3 - Journal article
C2 - 39793389
SN - 0304-3894
VL - 487
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 137136
ER -