Effects of pregnancy on obesity-induced inflammation in a mouse model of fetal programming

Camilla Ingvorsen, Anna Hammerich Thysen, D. Fernandez-Twinn, Pernille Nordby, Kristian Fog Nielsen, S. E. Ozanne, Susanne Brix Pedersen, Lars Hellgren

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Objective
    Maternal obesity is associated with increased risk of metabolic dysfunction in the offspring. It is not clear whether it is the metabolic changes or chronic low-grade inflammation in the obese state that causes this metabolic programming. We therefore investigated whether low-grade inflammation was present in obese dams compared to controls dams at gestation day 18.
    Methods
    Female mice were fed either a standard chow diet or a highly palatable obesogenic diet for 6 weeks prior to conception. Mice were either euthanized before mating (n=12 in each group), or euthanized on gestation day 18 (n=8 in each group). Blood and tissues were collected for analysis.
    Results
    The obesogenic diet increased body weight and decreased insulin sensitivity prior to conception, while there was no difference between the groups at gestation day 18. Local inflammation was assayed by macrophage count in adipose tissue and liver. Macrophage count in the adipose tissue was increased significantly by the obesogenic diet, and the hepatic count also showed a tendency to increased macrophage infiltration prior to gestation. This was further supported by a decreased population of monocytes in the blood of the obese animals, which suggested that monocytes are being recruited from the blood to the liver and adipose tissue in the obese animals. Gestation reversed macrophage infiltration, such that obese dams showed a lower adipose tissue macrophage count at the end of gestation compared to pre-pregnancy obese mice, and there were no longer a tendency towards increased hepatic macrophage count. Placental macrophage count was also similar in the two groups.
    Conclusion
    At gestation day 18, obese dams were found to have similar macrophage infiltration in placenta, adipose tissue and liver as lean dams, despite an incipient infiltration before gestation. Thus, the obesity-induced inflammation was reversed during gestation.
    Original languageEnglish
    JournalInternational Journal of Obesity
    Volume38
    Issue number10
    Pages (from-to)1282-1289
    ISSN0307-0565
    DOIs
    Publication statusPublished - 2014

    Cite this

    Ingvorsen, C., Thysen, A. H., Fernandez-Twinn, D., Nordby, P., Nielsen, K. F., Ozanne, S. E., ... Hellgren, L. (2014). Effects of pregnancy on obesity-induced inflammation in a mouse model of fetal programming. International Journal of Obesity, 38(10), 1282-1289. https://doi.org/10.1038/ijo.2014.69
    Ingvorsen, Camilla ; Thysen, Anna Hammerich ; Fernandez-Twinn, D. ; Nordby, Pernille ; Nielsen, Kristian Fog ; Ozanne, S. E. ; Pedersen, Susanne Brix ; Hellgren, Lars. / Effects of pregnancy on obesity-induced inflammation in a mouse model of fetal programming. In: International Journal of Obesity. 2014 ; Vol. 38, No. 10. pp. 1282-1289.
    @article{62c6312c3cd44e6e97d5b252d94d4480,
    title = "Effects of pregnancy on obesity-induced inflammation in a mouse model of fetal programming",
    abstract = "ObjectiveMaternal obesity is associated with increased risk of metabolic dysfunction in the offspring. It is not clear whether it is the metabolic changes or chronic low-grade inflammation in the obese state that causes this metabolic programming. We therefore investigated whether low-grade inflammation was present in obese dams compared to controls dams at gestation day 18.MethodsFemale mice were fed either a standard chow diet or a highly palatable obesogenic diet for 6 weeks prior to conception. Mice were either euthanized before mating (n=12 in each group), or euthanized on gestation day 18 (n=8 in each group). Blood and tissues were collected for analysis.ResultsThe obesogenic diet increased body weight and decreased insulin sensitivity prior to conception, while there was no difference between the groups at gestation day 18. Local inflammation was assayed by macrophage count in adipose tissue and liver. Macrophage count in the adipose tissue was increased significantly by the obesogenic diet, and the hepatic count also showed a tendency to increased macrophage infiltration prior to gestation. This was further supported by a decreased population of monocytes in the blood of the obese animals, which suggested that monocytes are being recruited from the blood to the liver and adipose tissue in the obese animals. Gestation reversed macrophage infiltration, such that obese dams showed a lower adipose tissue macrophage count at the end of gestation compared to pre-pregnancy obese mice, and there were no longer a tendency towards increased hepatic macrophage count. Placental macrophage count was also similar in the two groups.ConclusionAt gestation day 18, obese dams were found to have similar macrophage infiltration in placenta, adipose tissue and liver as lean dams, despite an incipient infiltration before gestation. Thus, the obesity-induced inflammation was reversed during gestation.",
    author = "Camilla Ingvorsen and Thysen, {Anna Hammerich} and D. Fernandez-Twinn and Pernille Nordby and Nielsen, {Kristian Fog} and Ozanne, {S. E.} and Pedersen, {Susanne Brix} and Lars Hellgren",
    year = "2014",
    doi = "10.1038/ijo.2014.69",
    language = "English",
    volume = "38",
    pages = "1282--1289",
    journal = "International Journal of Obesity",
    issn = "0307-0565",
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    Ingvorsen, C, Thysen, AH, Fernandez-Twinn, D, Nordby, P, Nielsen, KF, Ozanne, SE, Pedersen, SB & Hellgren, L 2014, 'Effects of pregnancy on obesity-induced inflammation in a mouse model of fetal programming', International Journal of Obesity, vol. 38, no. 10, pp. 1282-1289. https://doi.org/10.1038/ijo.2014.69

    Effects of pregnancy on obesity-induced inflammation in a mouse model of fetal programming. / Ingvorsen, Camilla; Thysen, Anna Hammerich; Fernandez-Twinn, D.; Nordby, Pernille; Nielsen, Kristian Fog; Ozanne, S. E.; Pedersen, Susanne Brix; Hellgren, Lars.

    In: International Journal of Obesity, Vol. 38, No. 10, 2014, p. 1282-1289.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Effects of pregnancy on obesity-induced inflammation in a mouse model of fetal programming

    AU - Ingvorsen, Camilla

    AU - Thysen, Anna Hammerich

    AU - Fernandez-Twinn, D.

    AU - Nordby, Pernille

    AU - Nielsen, Kristian Fog

    AU - Ozanne, S. E.

    AU - Pedersen, Susanne Brix

    AU - Hellgren, Lars

    PY - 2014

    Y1 - 2014

    N2 - ObjectiveMaternal obesity is associated with increased risk of metabolic dysfunction in the offspring. It is not clear whether it is the metabolic changes or chronic low-grade inflammation in the obese state that causes this metabolic programming. We therefore investigated whether low-grade inflammation was present in obese dams compared to controls dams at gestation day 18.MethodsFemale mice were fed either a standard chow diet or a highly palatable obesogenic diet for 6 weeks prior to conception. Mice were either euthanized before mating (n=12 in each group), or euthanized on gestation day 18 (n=8 in each group). Blood and tissues were collected for analysis.ResultsThe obesogenic diet increased body weight and decreased insulin sensitivity prior to conception, while there was no difference between the groups at gestation day 18. Local inflammation was assayed by macrophage count in adipose tissue and liver. Macrophage count in the adipose tissue was increased significantly by the obesogenic diet, and the hepatic count also showed a tendency to increased macrophage infiltration prior to gestation. This was further supported by a decreased population of monocytes in the blood of the obese animals, which suggested that monocytes are being recruited from the blood to the liver and adipose tissue in the obese animals. Gestation reversed macrophage infiltration, such that obese dams showed a lower adipose tissue macrophage count at the end of gestation compared to pre-pregnancy obese mice, and there were no longer a tendency towards increased hepatic macrophage count. Placental macrophage count was also similar in the two groups.ConclusionAt gestation day 18, obese dams were found to have similar macrophage infiltration in placenta, adipose tissue and liver as lean dams, despite an incipient infiltration before gestation. Thus, the obesity-induced inflammation was reversed during gestation.

    AB - ObjectiveMaternal obesity is associated with increased risk of metabolic dysfunction in the offspring. It is not clear whether it is the metabolic changes or chronic low-grade inflammation in the obese state that causes this metabolic programming. We therefore investigated whether low-grade inflammation was present in obese dams compared to controls dams at gestation day 18.MethodsFemale mice were fed either a standard chow diet or a highly palatable obesogenic diet for 6 weeks prior to conception. Mice were either euthanized before mating (n=12 in each group), or euthanized on gestation day 18 (n=8 in each group). Blood and tissues were collected for analysis.ResultsThe obesogenic diet increased body weight and decreased insulin sensitivity prior to conception, while there was no difference between the groups at gestation day 18. Local inflammation was assayed by macrophage count in adipose tissue and liver. Macrophage count in the adipose tissue was increased significantly by the obesogenic diet, and the hepatic count also showed a tendency to increased macrophage infiltration prior to gestation. This was further supported by a decreased population of monocytes in the blood of the obese animals, which suggested that monocytes are being recruited from the blood to the liver and adipose tissue in the obese animals. Gestation reversed macrophage infiltration, such that obese dams showed a lower adipose tissue macrophage count at the end of gestation compared to pre-pregnancy obese mice, and there were no longer a tendency towards increased hepatic macrophage count. Placental macrophage count was also similar in the two groups.ConclusionAt gestation day 18, obese dams were found to have similar macrophage infiltration in placenta, adipose tissue and liver as lean dams, despite an incipient infiltration before gestation. Thus, the obesity-induced inflammation was reversed during gestation.

    U2 - 10.1038/ijo.2014.69

    DO - 10.1038/ijo.2014.69

    M3 - Journal article

    VL - 38

    SP - 1282

    EP - 1289

    JO - International Journal of Obesity

    JF - International Journal of Obesity

    SN - 0307-0565

    IS - 10

    ER -