TY - JOUR
T1 - Effects of natural starch-phosphate monoester content on the multi-scale structures of potato starches
AU - Ding, Li
AU - Liang, Wenxin
AU - Qu, Jianzhou
AU - Persson, Staffan
AU - Liu, Xingxun
AU - Herburger, Klaus
AU - Kirkensgaard, Jacob Judas Kain
AU - Khakimov, Bekzod
AU - Enemark-Rasmussen, Kasper
AU - Blennow, Andreas
AU - Zhong, Yuyue
PY - 2023
Y1 - 2023
N2 - Starch phosphate content (SPC) is an important structural parameter affecting the functionality of tuber starches, but how different phosphate groups affect starch structures is still unknown. In this study, four potato starches with different SPC were selected as models to investigate the effects of SPC on their multi-scale structures. A higher SPC is related to more long amylopectin (AP) chains with a degree of polymerization (DP) >24, fewer short AP chains with DP ≤ 24, lower double helical content and crystallinity, higher long period distance, and lower scattering intensity (Imax), indicating that potato starch with a higher SPC is less ordered and has a more flexible nano-lamellar structure. Notably, two types of phosphate monoesters, namely C-3 (C3P) and C-6 phosphate monoesters (C6P), were both significantly positively correlated with the average chain lengths (ACL) of AP chains with DP 25–36 (fb2) and negatively correlated with Imax; however, only C3P was significantly positively correlated with ACL of AP chains with DP 13–24 (fb1) and negatively correlated with the absorbance ratio at 1047/1016 cm− 1. C3P was the main reason for the disordered structure of starches with high SPC. These findings can be helpful for potato breeding to generate different functionality by controlling SPC.
AB - Starch phosphate content (SPC) is an important structural parameter affecting the functionality of tuber starches, but how different phosphate groups affect starch structures is still unknown. In this study, four potato starches with different SPC were selected as models to investigate the effects of SPC on their multi-scale structures. A higher SPC is related to more long amylopectin (AP) chains with a degree of polymerization (DP) >24, fewer short AP chains with DP ≤ 24, lower double helical content and crystallinity, higher long period distance, and lower scattering intensity (Imax), indicating that potato starch with a higher SPC is less ordered and has a more flexible nano-lamellar structure. Notably, two types of phosphate monoesters, namely C-3 (C3P) and C-6 phosphate monoesters (C6P), were both significantly positively correlated with the average chain lengths (ACL) of AP chains with DP 25–36 (fb2) and negatively correlated with Imax; however, only C3P was significantly positively correlated with ACL of AP chains with DP 13–24 (fb1) and negatively correlated with the absorbance ratio at 1047/1016 cm− 1. C3P was the main reason for the disordered structure of starches with high SPC. These findings can be helpful for potato breeding to generate different functionality by controlling SPC.
KW - Amylose content
KW - Crystalline structure
KW - Lamellar structure
KW - Solid-state NMR
KW - Starch phosphate monoesters
KW - Starch phosphorylation
U2 - 10.1016/j.carbpol.2023.120740
DO - 10.1016/j.carbpol.2023.120740
M3 - Journal article
C2 - 36925255
SN - 0144-8617
VL - 310
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 120740
ER -