Effects of Lactococcus lactis on composition of intestinal microbiota: Role of nisin

Nete Bernbom, Tine Rask Licht, Carl-Henrik Brogren, Birthe Jelle, Anette H. Johansen, Iker Badiola, Finn K. Vogensen, Birgit Nørrung

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

This study examined the ability of (i) pure nisin, (ii) nisin-producing Lactococcus lactis strain CHCC5826, and (iii) the non-nisin-producing L. lactis strain CHCH2862 to affect the composition of the intestinal microbiota of human flora-associated rats. The presence of both the nisin-producing and the non-nisin-producing L. lactis strains significantly increased the number of Bifidobacterium cells in fecal samples during the first 8 days but decreased the number of enterococci/streptococci in duodenum, ileum, cecum, and colon samples as detected by selective cultivation. No significant changes in the rat fecal microbiota were observed after dosage with nisin. Pearson cluster analysis of denaturing gradient gel electrophoresis profiles of the 16S rRNA genes present in the fecal microbial population revealed that the microbiota of animals dosed with either of the two L. lactis strains were different from that of control animals dosed with saline. However, profiles of the microbiota from animals dosed with nisin did not differ from the controls. The concentrations of nisin estimated by competitive enzyme-linked immunosorbent assay (ELISA) were approximately 10-fold higher in the small intestine and 200-fold higher in feces than the corresponding concentrations estimated by a biological assay. This indicates that nisin was degraded or inactivated in the gastrointestinal tract, since fragments of this bacteriocin are detected by ELISA while an intact molecule is needed to retain biological activity.
Original languageEnglish
JournalApplied and Environmental Microbiology
Volume72
Issue number1
Pages (from-to)239-244
ISSN0099-2240
DOIs
Publication statusPublished - 2006

Cite this

Bernbom, Nete ; Licht, Tine Rask ; Brogren, Carl-Henrik ; Jelle, Birthe ; Johansen, Anette H. ; Badiola, Iker ; Vogensen, Finn K. ; Nørrung, Birgit. / Effects of Lactococcus lactis on composition of intestinal microbiota: Role of nisin. In: Applied and Environmental Microbiology. 2006 ; Vol. 72, No. 1. pp. 239-244.
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title = "Effects of Lactococcus lactis on composition of intestinal microbiota: Role of nisin",
abstract = "This study examined the ability of (i) pure nisin, (ii) nisin-producing Lactococcus lactis strain CHCC5826, and (iii) the non-nisin-producing L. lactis strain CHCH2862 to affect the composition of the intestinal microbiota of human flora-associated rats. The presence of both the nisin-producing and the non-nisin-producing L. lactis strains significantly increased the number of Bifidobacterium cells in fecal samples during the first 8 days but decreased the number of enterococci/streptococci in duodenum, ileum, cecum, and colon samples as detected by selective cultivation. No significant changes in the rat fecal microbiota were observed after dosage with nisin. Pearson cluster analysis of denaturing gradient gel electrophoresis profiles of the 16S rRNA genes present in the fecal microbial population revealed that the microbiota of animals dosed with either of the two L. lactis strains were different from that of control animals dosed with saline. However, profiles of the microbiota from animals dosed with nisin did not differ from the controls. The concentrations of nisin estimated by competitive enzyme-linked immunosorbent assay (ELISA) were approximately 10-fold higher in the small intestine and 200-fold higher in feces than the corresponding concentrations estimated by a biological assay. This indicates that nisin was degraded or inactivated in the gastrointestinal tract, since fragments of this bacteriocin are detected by ELISA while an intact molecule is needed to retain biological activity.",
author = "Nete Bernbom and Licht, {Tine Rask} and Carl-Henrik Brogren and Birthe Jelle and Johansen, {Anette H.} and Iker Badiola and Vogensen, {Finn K.} and Birgit N{\o}rrung",
year = "2006",
doi = "10.1128/AEM.72.1.239-244.2006",
language = "English",
volume = "72",
pages = "239--244",
journal = "Applied and Environmental Microbiology",
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Bernbom, N, Licht, TR, Brogren, C-H, Jelle, B, Johansen, AH, Badiola, I, Vogensen, FK & Nørrung, B 2006, 'Effects of Lactococcus lactis on composition of intestinal microbiota: Role of nisin', Applied and Environmental Microbiology, vol. 72, no. 1, pp. 239-244. https://doi.org/10.1128/AEM.72.1.239-244.2006

Effects of Lactococcus lactis on composition of intestinal microbiota: Role of nisin. / Bernbom, Nete; Licht, Tine Rask; Brogren, Carl-Henrik; Jelle, Birthe; Johansen, Anette H.; Badiola, Iker; Vogensen, Finn K.; Nørrung, Birgit.

In: Applied and Environmental Microbiology, Vol. 72, No. 1, 2006, p. 239-244.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Effects of Lactococcus lactis on composition of intestinal microbiota: Role of nisin

AU - Bernbom, Nete

AU - Licht, Tine Rask

AU - Brogren, Carl-Henrik

AU - Jelle, Birthe

AU - Johansen, Anette H.

AU - Badiola, Iker

AU - Vogensen, Finn K.

AU - Nørrung, Birgit

PY - 2006

Y1 - 2006

N2 - This study examined the ability of (i) pure nisin, (ii) nisin-producing Lactococcus lactis strain CHCC5826, and (iii) the non-nisin-producing L. lactis strain CHCH2862 to affect the composition of the intestinal microbiota of human flora-associated rats. The presence of both the nisin-producing and the non-nisin-producing L. lactis strains significantly increased the number of Bifidobacterium cells in fecal samples during the first 8 days but decreased the number of enterococci/streptococci in duodenum, ileum, cecum, and colon samples as detected by selective cultivation. No significant changes in the rat fecal microbiota were observed after dosage with nisin. Pearson cluster analysis of denaturing gradient gel electrophoresis profiles of the 16S rRNA genes present in the fecal microbial population revealed that the microbiota of animals dosed with either of the two L. lactis strains were different from that of control animals dosed with saline. However, profiles of the microbiota from animals dosed with nisin did not differ from the controls. The concentrations of nisin estimated by competitive enzyme-linked immunosorbent assay (ELISA) were approximately 10-fold higher in the small intestine and 200-fold higher in feces than the corresponding concentrations estimated by a biological assay. This indicates that nisin was degraded or inactivated in the gastrointestinal tract, since fragments of this bacteriocin are detected by ELISA while an intact molecule is needed to retain biological activity.

AB - This study examined the ability of (i) pure nisin, (ii) nisin-producing Lactococcus lactis strain CHCC5826, and (iii) the non-nisin-producing L. lactis strain CHCH2862 to affect the composition of the intestinal microbiota of human flora-associated rats. The presence of both the nisin-producing and the non-nisin-producing L. lactis strains significantly increased the number of Bifidobacterium cells in fecal samples during the first 8 days but decreased the number of enterococci/streptococci in duodenum, ileum, cecum, and colon samples as detected by selective cultivation. No significant changes in the rat fecal microbiota were observed after dosage with nisin. Pearson cluster analysis of denaturing gradient gel electrophoresis profiles of the 16S rRNA genes present in the fecal microbial population revealed that the microbiota of animals dosed with either of the two L. lactis strains were different from that of control animals dosed with saline. However, profiles of the microbiota from animals dosed with nisin did not differ from the controls. The concentrations of nisin estimated by competitive enzyme-linked immunosorbent assay (ELISA) were approximately 10-fold higher in the small intestine and 200-fold higher in feces than the corresponding concentrations estimated by a biological assay. This indicates that nisin was degraded or inactivated in the gastrointestinal tract, since fragments of this bacteriocin are detected by ELISA while an intact molecule is needed to retain biological activity.

U2 - 10.1128/AEM.72.1.239-244.2006

DO - 10.1128/AEM.72.1.239-244.2006

M3 - Journal article

VL - 72

SP - 239

EP - 244

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 1

ER -