Effects of Iron on DNA Release and Biofilm Development by Pseudomonas Aeruginosa

Liang Yang, Kim Bundvig Barken, Mette Elena Skindersø, Allan Beck Christensen, Michael Christian Givskov, Tim Tolker-Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Extracellular DNA is one of the major matrix components in Pseudomonas aeruginosa biofilms. It functions as an intercellular connector and plays a role in stabilization of the biofilms. Evidence that DNA release in P. aeruginosa PAO1 biofilms is controlled by the las-rhl and pqs quorum-sensing systems has been previously presented. This paper provides evidence that DNA release in P. aeruginosa PAO1 biofilms is also under iron regulation. Experiments involving cultivation of P. aeruginosa in microtitre trays suggested that pqs expression, DNA release and biofilm formation were favoured in media with low iron concentrations (5 mu M FeCIA and decreased with increasing iron concentrations. Experiments involving cultivation of P. aeruginosa in a flow-chamber system suggested that a high level of iron (1100 mu M FeCl3) in the medium suppressed DNA release, structural biofilm development, and the development of subpopulations with increased tolerance toward antimicrobial compounds. Experiments with P. aeruginosa strains harbouring fluorescent reporters suggested that expression of the pqs operon was induced in particular subpopulations of the biofilm cells under low-iron conditions (1 mu M FeCl3) but repressed in the biofilm cells under high-iron conditions (100 mu M FeCl3).
    Original languageEnglish
    JournalMicrobiology
    Volume153
    Issue number5
    Pages (from-to)1318-1328
    ISSN1350-0872
    DOIs
    Publication statusPublished - 2007

    Cite this

    Yang, L., Barken, K. B., Skindersø, M. E., Christensen, A. B., Givskov, M. C., & Tolker-Nielsen, T. (2007). Effects of Iron on DNA Release and Biofilm Development by Pseudomonas Aeruginosa. Microbiology, 153(5), 1318-1328. https://doi.org/10.1099/mic.0.2006/004911-0
    Yang, Liang ; Barken, Kim Bundvig ; Skindersø, Mette Elena ; Christensen, Allan Beck ; Givskov, Michael Christian ; Tolker-Nielsen, Tim. / Effects of Iron on DNA Release and Biofilm Development by Pseudomonas Aeruginosa. In: Microbiology. 2007 ; Vol. 153, No. 5. pp. 1318-1328.
    @article{d85126bf69b04f9cb86560d4c40f8071,
    title = "Effects of Iron on DNA Release and Biofilm Development by Pseudomonas Aeruginosa",
    abstract = "Extracellular DNA is one of the major matrix components in Pseudomonas aeruginosa biofilms. It functions as an intercellular connector and plays a role in stabilization of the biofilms. Evidence that DNA release in P. aeruginosa PAO1 biofilms is controlled by the las-rhl and pqs quorum-sensing systems has been previously presented. This paper provides evidence that DNA release in P. aeruginosa PAO1 biofilms is also under iron regulation. Experiments involving cultivation of P. aeruginosa in microtitre trays suggested that pqs expression, DNA release and biofilm formation were favoured in media with low iron concentrations (5 mu M FeCIA and decreased with increasing iron concentrations. Experiments involving cultivation of P. aeruginosa in a flow-chamber system suggested that a high level of iron (1100 mu M FeCl3) in the medium suppressed DNA release, structural biofilm development, and the development of subpopulations with increased tolerance toward antimicrobial compounds. Experiments with P. aeruginosa strains harbouring fluorescent reporters suggested that expression of the pqs operon was induced in particular subpopulations of the biofilm cells under low-iron conditions (1 mu M FeCl3) but repressed in the biofilm cells under high-iron conditions (100 mu M FeCl3).",
    author = "Liang Yang and Barken, {Kim Bundvig} and Skinders{\o}, {Mette Elena} and Christensen, {Allan Beck} and Givskov, {Michael Christian} and Tim Tolker-Nielsen",
    year = "2007",
    doi = "10.1099/mic.0.2006/004911-0",
    language = "English",
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    Yang, L, Barken, KB, Skindersø, ME, Christensen, AB, Givskov, MC & Tolker-Nielsen, T 2007, 'Effects of Iron on DNA Release and Biofilm Development by Pseudomonas Aeruginosa', Microbiology, vol. 153, no. 5, pp. 1318-1328. https://doi.org/10.1099/mic.0.2006/004911-0

    Effects of Iron on DNA Release and Biofilm Development by Pseudomonas Aeruginosa. / Yang, Liang; Barken, Kim Bundvig; Skindersø, Mette Elena; Christensen, Allan Beck; Givskov, Michael Christian; Tolker-Nielsen, Tim.

    In: Microbiology, Vol. 153, No. 5, 2007, p. 1318-1328.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Effects of Iron on DNA Release and Biofilm Development by Pseudomonas Aeruginosa

    AU - Yang, Liang

    AU - Barken, Kim Bundvig

    AU - Skindersø, Mette Elena

    AU - Christensen, Allan Beck

    AU - Givskov, Michael Christian

    AU - Tolker-Nielsen, Tim

    PY - 2007

    Y1 - 2007

    N2 - Extracellular DNA is one of the major matrix components in Pseudomonas aeruginosa biofilms. It functions as an intercellular connector and plays a role in stabilization of the biofilms. Evidence that DNA release in P. aeruginosa PAO1 biofilms is controlled by the las-rhl and pqs quorum-sensing systems has been previously presented. This paper provides evidence that DNA release in P. aeruginosa PAO1 biofilms is also under iron regulation. Experiments involving cultivation of P. aeruginosa in microtitre trays suggested that pqs expression, DNA release and biofilm formation were favoured in media with low iron concentrations (5 mu M FeCIA and decreased with increasing iron concentrations. Experiments involving cultivation of P. aeruginosa in a flow-chamber system suggested that a high level of iron (1100 mu M FeCl3) in the medium suppressed DNA release, structural biofilm development, and the development of subpopulations with increased tolerance toward antimicrobial compounds. Experiments with P. aeruginosa strains harbouring fluorescent reporters suggested that expression of the pqs operon was induced in particular subpopulations of the biofilm cells under low-iron conditions (1 mu M FeCl3) but repressed in the biofilm cells under high-iron conditions (100 mu M FeCl3).

    AB - Extracellular DNA is one of the major matrix components in Pseudomonas aeruginosa biofilms. It functions as an intercellular connector and plays a role in stabilization of the biofilms. Evidence that DNA release in P. aeruginosa PAO1 biofilms is controlled by the las-rhl and pqs quorum-sensing systems has been previously presented. This paper provides evidence that DNA release in P. aeruginosa PAO1 biofilms is also under iron regulation. Experiments involving cultivation of P. aeruginosa in microtitre trays suggested that pqs expression, DNA release and biofilm formation were favoured in media with low iron concentrations (5 mu M FeCIA and decreased with increasing iron concentrations. Experiments involving cultivation of P. aeruginosa in a flow-chamber system suggested that a high level of iron (1100 mu M FeCl3) in the medium suppressed DNA release, structural biofilm development, and the development of subpopulations with increased tolerance toward antimicrobial compounds. Experiments with P. aeruginosa strains harbouring fluorescent reporters suggested that expression of the pqs operon was induced in particular subpopulations of the biofilm cells under low-iron conditions (1 mu M FeCl3) but repressed in the biofilm cells under high-iron conditions (100 mu M FeCl3).

    U2 - 10.1099/mic.0.2006/004911-0

    DO - 10.1099/mic.0.2006/004911-0

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    VL - 153

    SP - 1318

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    JO - Microbiology

    JF - Microbiology

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    IS - 5

    ER -

    Yang L, Barken KB, Skindersø ME, Christensen AB, Givskov MC, Tolker-Nielsen T. Effects of Iron on DNA Release and Biofilm Development by Pseudomonas Aeruginosa. Microbiology. 2007;153(5):1318-1328. https://doi.org/10.1099/mic.0.2006/004911-0