Effects of fish size and route of infection on virulence of a Danish Yersinia ruckeri O1 biotype 2 strain in rainbow trout (Oncorhynchus mykiss)

Maki Ohtani, Kasper Rømer Villumsen, Helene Kragelund Strøm, Anne Hjørngaard Lauritsen, Bent Aalbæk, Inger Dalsgaard, Barbara Nowak, Martin Kristian Raida, Anders Miki Bojesen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The comparative virulence of Yersinia ruckeri serotype O1 biotype 2 strain 07111224 from a Danish outbreak of enteric redmouth disease in rainbow trout (Oncorhynchus mykiss) was investigated in different sizes of rainbow trout (5, 19, 25 and 52 g) using a panel of challenge methods (bath, intraperitoneal (IP) injection, anal intubation and cohabitation). The results show that Y. ruckeri 07111224 is virulent enough to successfully cause mortality in 5–52 g rainbow trout through three different infection routes (bath, IP injection and anal intubation), and strongly suggests that susceptibility to Y. ruckeri infection is dependent on fish size. Bath challenge (108 CFU/ml) caused 79% mortality in 5 g fish, while the mortality in 19–52 g fish was approximately 26%. IP injection challenges (105–106 CFU/individual) resulted in high mortalites in fish of all sizes. Anal intubation challenges (109 CFU/individual) induced 82% mortality in 5 g fish and decreased with increasing fish weight. Finally, cohabitation challenges induced 45% mortality in 5 g test fish when test fish were cohabitated 1:1 with shedder fish, while mortalities in test fish of five other challenge groups were lower (5–25%). Statistical models were fit to the data from each challenge model. The model parameter predictions suggests statistically significant effects of fish weight for bath, IP and anal intubation challenges. Furthermore, they highlight the importance of the ratio of shedders to test fish in cohabitation challenges and of the challenge doses, in particular for the IP challenge model.

Original languageEnglish
JournalAquaculture
Volume503
Pages (from-to)519-526
ISSN0044-8486
DOIs
Publication statusPublished - 2019

Keywords

  • Anal intubation
  • Bath
  • Cohabitation
  • Intraperitoneal injection
  • Yersinia ruckeri O1 biotype 2

Cite this

Ohtani, Maki ; Villumsen, Kasper Rømer ; Strøm, Helene Kragelund ; Lauritsen, Anne Hjørngaard ; Aalbæk, Bent ; Dalsgaard, Inger ; Nowak, Barbara ; Raida, Martin Kristian ; Bojesen, Anders Miki. / Effects of fish size and route of infection on virulence of a Danish Yersinia ruckeri O1 biotype 2 strain in rainbow trout (Oncorhynchus mykiss). In: Aquaculture. 2019 ; Vol. 503. pp. 519-526.
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title = "Effects of fish size and route of infection on virulence of a Danish Yersinia ruckeri O1 biotype 2 strain in rainbow trout (Oncorhynchus mykiss)",
abstract = "The comparative virulence of Yersinia ruckeri serotype O1 biotype 2 strain 07111224 from a Danish outbreak of enteric redmouth disease in rainbow trout (Oncorhynchus mykiss) was investigated in different sizes of rainbow trout (5, 19, 25 and 52 g) using a panel of challenge methods (bath, intraperitoneal (IP) injection, anal intubation and cohabitation). The results show that Y. ruckeri 07111224 is virulent enough to successfully cause mortality in 5–52 g rainbow trout through three different infection routes (bath, IP injection and anal intubation), and strongly suggests that susceptibility to Y. ruckeri infection is dependent on fish size. Bath challenge (108 CFU/ml) caused 79{\%} mortality in 5 g fish, while the mortality in 19–52 g fish was approximately 26{\%}. IP injection challenges (105–106 CFU/individual) resulted in high mortalites in fish of all sizes. Anal intubation challenges (109 CFU/individual) induced 82{\%} mortality in 5 g fish and decreased with increasing fish weight. Finally, cohabitation challenges induced 45{\%} mortality in 5 g test fish when test fish were cohabitated 1:1 with shedder fish, while mortalities in test fish of five other challenge groups were lower (5–25{\%}). Statistical models were fit to the data from each challenge model. The model parameter predictions suggests statistically significant effects of fish weight for bath, IP and anal intubation challenges. Furthermore, they highlight the importance of the ratio of shedders to test fish in cohabitation challenges and of the challenge doses, in particular for the IP challenge model.",
keywords = "Anal intubation, Bath, Cohabitation, Intraperitoneal injection, Yersinia ruckeri O1 biotype 2",
author = "Maki Ohtani and Villumsen, {Kasper R{\o}mer} and Str{\o}m, {Helene Kragelund} and Lauritsen, {Anne Hj{\o}rngaard} and Bent Aalb{\ae}k and Inger Dalsgaard and Barbara Nowak and Raida, {Martin Kristian} and Bojesen, {Anders Miki}",
year = "2019",
doi = "10.1016/j.aquaculture.2019.01.041",
language = "English",
volume = "503",
pages = "519--526",
journal = "Aquaculture",
issn = "0044-8486",
publisher = "Elsevier",

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Effects of fish size and route of infection on virulence of a Danish Yersinia ruckeri O1 biotype 2 strain in rainbow trout (Oncorhynchus mykiss). / Ohtani, Maki; Villumsen, Kasper Rømer; Strøm, Helene Kragelund; Lauritsen, Anne Hjørngaard; Aalbæk, Bent; Dalsgaard, Inger; Nowak, Barbara; Raida, Martin Kristian; Bojesen, Anders Miki.

In: Aquaculture, Vol. 503, 2019, p. 519-526.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Effects of fish size and route of infection on virulence of a Danish Yersinia ruckeri O1 biotype 2 strain in rainbow trout (Oncorhynchus mykiss)

AU - Ohtani, Maki

AU - Villumsen, Kasper Rømer

AU - Strøm, Helene Kragelund

AU - Lauritsen, Anne Hjørngaard

AU - Aalbæk, Bent

AU - Dalsgaard, Inger

AU - Nowak, Barbara

AU - Raida, Martin Kristian

AU - Bojesen, Anders Miki

PY - 2019

Y1 - 2019

N2 - The comparative virulence of Yersinia ruckeri serotype O1 biotype 2 strain 07111224 from a Danish outbreak of enteric redmouth disease in rainbow trout (Oncorhynchus mykiss) was investigated in different sizes of rainbow trout (5, 19, 25 and 52 g) using a panel of challenge methods (bath, intraperitoneal (IP) injection, anal intubation and cohabitation). The results show that Y. ruckeri 07111224 is virulent enough to successfully cause mortality in 5–52 g rainbow trout through three different infection routes (bath, IP injection and anal intubation), and strongly suggests that susceptibility to Y. ruckeri infection is dependent on fish size. Bath challenge (108 CFU/ml) caused 79% mortality in 5 g fish, while the mortality in 19–52 g fish was approximately 26%. IP injection challenges (105–106 CFU/individual) resulted in high mortalites in fish of all sizes. Anal intubation challenges (109 CFU/individual) induced 82% mortality in 5 g fish and decreased with increasing fish weight. Finally, cohabitation challenges induced 45% mortality in 5 g test fish when test fish were cohabitated 1:1 with shedder fish, while mortalities in test fish of five other challenge groups were lower (5–25%). Statistical models were fit to the data from each challenge model. The model parameter predictions suggests statistically significant effects of fish weight for bath, IP and anal intubation challenges. Furthermore, they highlight the importance of the ratio of shedders to test fish in cohabitation challenges and of the challenge doses, in particular for the IP challenge model.

AB - The comparative virulence of Yersinia ruckeri serotype O1 biotype 2 strain 07111224 from a Danish outbreak of enteric redmouth disease in rainbow trout (Oncorhynchus mykiss) was investigated in different sizes of rainbow trout (5, 19, 25 and 52 g) using a panel of challenge methods (bath, intraperitoneal (IP) injection, anal intubation and cohabitation). The results show that Y. ruckeri 07111224 is virulent enough to successfully cause mortality in 5–52 g rainbow trout through three different infection routes (bath, IP injection and anal intubation), and strongly suggests that susceptibility to Y. ruckeri infection is dependent on fish size. Bath challenge (108 CFU/ml) caused 79% mortality in 5 g fish, while the mortality in 19–52 g fish was approximately 26%. IP injection challenges (105–106 CFU/individual) resulted in high mortalites in fish of all sizes. Anal intubation challenges (109 CFU/individual) induced 82% mortality in 5 g fish and decreased with increasing fish weight. Finally, cohabitation challenges induced 45% mortality in 5 g test fish when test fish were cohabitated 1:1 with shedder fish, while mortalities in test fish of five other challenge groups were lower (5–25%). Statistical models were fit to the data from each challenge model. The model parameter predictions suggests statistically significant effects of fish weight for bath, IP and anal intubation challenges. Furthermore, they highlight the importance of the ratio of shedders to test fish in cohabitation challenges and of the challenge doses, in particular for the IP challenge model.

KW - Anal intubation

KW - Bath

KW - Cohabitation

KW - Intraperitoneal injection

KW - Yersinia ruckeri O1 biotype 2

U2 - 10.1016/j.aquaculture.2019.01.041

DO - 10.1016/j.aquaculture.2019.01.041

M3 - Journal article

VL - 503

SP - 519

EP - 526

JO - Aquaculture

JF - Aquaculture

SN - 0044-8486

ER -