Effects of dietary energy density and digestible protein:energy ratio on de novo lipid synthesis from dietary protein in gilthead sea bream (Sparus aurata) quantified with stable isotopes

Kim Schøn Ekmann, Anne Johanne Tang Dalsgaard, Jørgen Holm, Patrick J. Campbell, Peter Vilhelm Skov

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Abstract

The effects of varying dietary digestible protein (DP) and digestible energy (DE) content on performance, nutrient retention efficiency and the de novo lipogenesis of DP origin were examined in triplicate groups of gilthead sea bream (Sparus aurata), fed nine extruded experimental diets. In order to trace the metabolic fate of dietary protein, 1·8% fishmeal was replaced with isotope-labelled whole protein (.98% 13C). The experiment was divided into a growth period lasting 89 d, growing fish from approximately 140 to 350 g, followed by a 3 d period feeding isotope-enriched diets. Isotope ratio MS was applied to quantify the 13C enrichment of whole-body lipid from dietary DP. Between 18·6 and 22·4% of the carbon derived from protein was recovered in the lipid fraction of the fish, and between 21·6 and 30·3% of the total lipid deposited could be attributed to dietary protein. DP retention was significantly improved by reductions in dietary
DP:DE ratio, while the opposite was true for apparent digestible lipid retention. Both overall DE retention and whole-body proximate composition of whole fish were largely unaffected by dietary treatments, while feed conversion ratios were significantly improved with increasing dietary energy density. The present study suggests that gilthead sea bream efficiently utilises dietary nutrients over a wide
range of DP:DE ratios and energy densities. In addition, they appear to endeavour a certain body energy status rather than maximising growth, which in the present trial was apparent from inherently high de novo lipogenesis originating from DP
Original languageEnglish
JournalBritish Journal of Nutrition
Volume110
Pages (from-to)1771-1781
ISSN0007-1145
DOIs
Publication statusPublished - 2013

Cite this

@article{030a354faedc478db553506da0420ed3,
title = "Effects of dietary energy density and digestible protein:energy ratio on de novo lipid synthesis from dietary protein in gilthead sea bream (Sparus aurata) quantified with stable isotopes",
abstract = "The effects of varying dietary digestible protein (DP) and digestible energy (DE) content on performance, nutrient retention efficiency and the de novo lipogenesis of DP origin were examined in triplicate groups of gilthead sea bream (Sparus aurata), fed nine extruded experimental diets. In order to trace the metabolic fate of dietary protein, 1·8{\%} fishmeal was replaced with isotope-labelled whole protein (.98{\%} 13C). The experiment was divided into a growth period lasting 89 d, growing fish from approximately 140 to 350 g, followed by a 3 d period feeding isotope-enriched diets. Isotope ratio MS was applied to quantify the 13C enrichment of whole-body lipid from dietary DP. Between 18·6 and 22·4{\%} of the carbon derived from protein was recovered in the lipid fraction of the fish, and between 21·6 and 30·3{\%} of the total lipid deposited could be attributed to dietary protein. DP retention was significantly improved by reductions in dietary DP:DE ratio, while the opposite was true for apparent digestible lipid retention. Both overall DE retention and whole-body proximate composition of whole fish were largely unaffected by dietary treatments, while feed conversion ratios were significantly improved with increasing dietary energy density. The present study suggests that gilthead sea bream efficiently utilises dietary nutrients over a wide range of DP:DE ratios and energy densities. In addition, they appear to endeavour a certain body energy status rather than maximising growth, which in the present trial was apparent from inherently high de novo lipogenesis originating from DP",
author = "Ekmann, {Kim Sch{\o}n} and Dalsgaard, {Anne Johanne Tang} and J{\o}rgen Holm and Campbell, {Patrick J.} and Skov, {Peter Vilhelm}",
year = "2013",
doi = "10.1017/S0007114513001281",
language = "English",
volume = "110",
pages = "1771--1781",
journal = "British Journal of Nutrition",
issn = "0007-1145",
publisher = "Cambridge University Press",

}

Effects of dietary energy density and digestible protein:energy ratio on de novo lipid synthesis from dietary protein in gilthead sea bream (Sparus aurata) quantified with stable isotopes. / Ekmann, Kim Schøn; Dalsgaard, Anne Johanne Tang; Holm, Jørgen; Campbell, Patrick J.; Skov, Peter Vilhelm.

In: British Journal of Nutrition, Vol. 110, 2013, p. 1771-1781.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Effects of dietary energy density and digestible protein:energy ratio on de novo lipid synthesis from dietary protein in gilthead sea bream (Sparus aurata) quantified with stable isotopes

AU - Ekmann, Kim Schøn

AU - Dalsgaard, Anne Johanne Tang

AU - Holm, Jørgen

AU - Campbell, Patrick J.

AU - Skov, Peter Vilhelm

PY - 2013

Y1 - 2013

N2 - The effects of varying dietary digestible protein (DP) and digestible energy (DE) content on performance, nutrient retention efficiency and the de novo lipogenesis of DP origin were examined in triplicate groups of gilthead sea bream (Sparus aurata), fed nine extruded experimental diets. In order to trace the metabolic fate of dietary protein, 1·8% fishmeal was replaced with isotope-labelled whole protein (.98% 13C). The experiment was divided into a growth period lasting 89 d, growing fish from approximately 140 to 350 g, followed by a 3 d period feeding isotope-enriched diets. Isotope ratio MS was applied to quantify the 13C enrichment of whole-body lipid from dietary DP. Between 18·6 and 22·4% of the carbon derived from protein was recovered in the lipid fraction of the fish, and between 21·6 and 30·3% of the total lipid deposited could be attributed to dietary protein. DP retention was significantly improved by reductions in dietary DP:DE ratio, while the opposite was true for apparent digestible lipid retention. Both overall DE retention and whole-body proximate composition of whole fish were largely unaffected by dietary treatments, while feed conversion ratios were significantly improved with increasing dietary energy density. The present study suggests that gilthead sea bream efficiently utilises dietary nutrients over a wide range of DP:DE ratios and energy densities. In addition, they appear to endeavour a certain body energy status rather than maximising growth, which in the present trial was apparent from inherently high de novo lipogenesis originating from DP

AB - The effects of varying dietary digestible protein (DP) and digestible energy (DE) content on performance, nutrient retention efficiency and the de novo lipogenesis of DP origin were examined in triplicate groups of gilthead sea bream (Sparus aurata), fed nine extruded experimental diets. In order to trace the metabolic fate of dietary protein, 1·8% fishmeal was replaced with isotope-labelled whole protein (.98% 13C). The experiment was divided into a growth period lasting 89 d, growing fish from approximately 140 to 350 g, followed by a 3 d period feeding isotope-enriched diets. Isotope ratio MS was applied to quantify the 13C enrichment of whole-body lipid from dietary DP. Between 18·6 and 22·4% of the carbon derived from protein was recovered in the lipid fraction of the fish, and between 21·6 and 30·3% of the total lipid deposited could be attributed to dietary protein. DP retention was significantly improved by reductions in dietary DP:DE ratio, while the opposite was true for apparent digestible lipid retention. Both overall DE retention and whole-body proximate composition of whole fish were largely unaffected by dietary treatments, while feed conversion ratios were significantly improved with increasing dietary energy density. The present study suggests that gilthead sea bream efficiently utilises dietary nutrients over a wide range of DP:DE ratios and energy densities. In addition, they appear to endeavour a certain body energy status rather than maximising growth, which in the present trial was apparent from inherently high de novo lipogenesis originating from DP

U2 - 10.1017/S0007114513001281

DO - 10.1017/S0007114513001281

M3 - Journal article

VL - 110

SP - 1771

EP - 1781

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

ER -