TY - JOUR
T1 - Effects of carbon doping on the microstructural, micro/nano-mechanical, and mesenchymal stromal cells biocompatibility and osteogenic differentiation properties of alumina
AU - Krishnamurithy, Genasan
AU - Yahya, Noor Azlin
AU - Mehrali, Mehdi
AU - Mehrali, Mohammad
AU - Mohan, Saktiswaren
AU - Murali, Malliga Raman
AU - Raghavendran, Hanumantha Rao Balaji
AU - Kamarul, Tunku
PY - 2016
Y1 - 2016
N2 - It has been demonstrated that carbon (C) doped aluminium oxide (Al2O3) nanocomposite (C −0.012wt%) had greater wear resistance and lower surface grains pull out percentage when compared with monolithic Al2O3. In the present study, we investigated the physicochemical, micro- and nanomechanical, cell attachment, in vitro biocompatibility and osteogenic differentiation properties of Al2O3 doped carbon (0.012wt%) nanocomposite (Al2O3/C). Data were compared to values obtained for monolithic alumina (Al2O3). The calcined Al2O3/C nanocomposite was densified using cold isostatic pressing and followed by pressureless sintering. For physicochemical and microstructural characterisation, Energy dispersive X-ray (EDX), X-ray diffraction (XRD), Raman spectroscopy, and X-ray photoemission spectrometer (XPS) were used. EDX, XRD peaks and Raman spectroscopy demonstrated correlating to Al2O3/C. Surface profiling and contact angle investigations demonstrated highly contoured micro-surface topography. The micro and nano-hardness indicate an improved wear resistance of the Al2O3/C when compared with monolithic Al2O3. SEM, confocal images and alamar blue reduction assay suggested good cell attachment and proliferation of human bone marrow derived mesenchymal stromal cells (hBMSCs). Osteogenic protein and gene expression indicated Al2O3/C had a significant osteogenic potential (p
AB - It has been demonstrated that carbon (C) doped aluminium oxide (Al2O3) nanocomposite (C −0.012wt%) had greater wear resistance and lower surface grains pull out percentage when compared with monolithic Al2O3. In the present study, we investigated the physicochemical, micro- and nanomechanical, cell attachment, in vitro biocompatibility and osteogenic differentiation properties of Al2O3 doped carbon (0.012wt%) nanocomposite (Al2O3/C). Data were compared to values obtained for monolithic alumina (Al2O3). The calcined Al2O3/C nanocomposite was densified using cold isostatic pressing and followed by pressureless sintering. For physicochemical and microstructural characterisation, Energy dispersive X-ray (EDX), X-ray diffraction (XRD), Raman spectroscopy, and X-ray photoemission spectrometer (XPS) were used. EDX, XRD peaks and Raman spectroscopy demonstrated correlating to Al2O3/C. Surface profiling and contact angle investigations demonstrated highly contoured micro-surface topography. The micro and nano-hardness indicate an improved wear resistance of the Al2O3/C when compared with monolithic Al2O3. SEM, confocal images and alamar blue reduction assay suggested good cell attachment and proliferation of human bone marrow derived mesenchymal stromal cells (hBMSCs). Osteogenic protein and gene expression indicated Al2O3/C had a significant osteogenic potential (p
KW - Al2O3
KW - Carbon
KW - Microstructure-final
KW - Hardness
KW - Mechanical properties
KW - Biomedical applications
U2 - 10.1016/j.ceramint.2016.08.148
DO - 10.1016/j.ceramint.2016.08.148
M3 - Journal article
SN - 0272-8842
VL - 42
SP - 18247
EP - 18256
JO - Ceramics International
JF - Ceramics International
IS - 16
ER -