Effects of Aeration on the Synthesis of Poly(3-Hydroxybutyrate) from Glycerol and Glucose in Recombinant Escherichia coli

Alejandra De Almeida, Andrea M. Giordano, Pablo Ivan Nikel, M. Julia Pettinari

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Abstract

Bioreactor cultures of Escherichia coli recombinants carrying phaBAC and phaP of Azotobacter sp. FA8 grown on glycerol under low-agitation conditions accumulated more poly(3-hydroxybutyrate) (PHB) and ethanol than at high agitation, while in glucose cultures, low agitation led to a decrease in PHB formation. Cells produced smaller amounts of acids from glycerol than from glucose. Glycerol batch cultures stirred at 125 rpm accumulated, in 24 h, 30.1% (wt/wt) PHB with a relative molecular mass of 1.9 MDa, close to that of PHB obtained using glucose.
Original languageEnglish
JournalApplied and Environmental Microbiology
Volume76
Issue number6
Pages (from-to)2036-2040
Number of pages5
ISSN0099-2240
DOIs
Publication statusPublished - 2010
Externally publishedYes

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