Effects of acute and prolonged naphthalene exposure on brain monoaminergic neurotransmitters in rainbow trout (Oncorhynchus mykiss)

Manuel Gesto, Adrian Tintos, Jose L. Soengas, Jesus M. Miguez

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We have shown previously that acute (I to 6 h) and prolonged (I to 5 days) exposure of rainbow trout to naphthalene resulted in decreased plasmatic cortisol and 17-beta-estradiol levels. In order to elucidate the mechanisms through which naphthalene might disrupt endocrine regulation, the present study investigated whether brain monoaminergic neurotransmitters are altered by the action of this polycyclic aromatic hydrocarbon. In a first experiment, immature rainbow trout were injected with vegetable oil alone or containing naphthalene (10 and 50 mg/kg, i.p.), and sacrificed 1, 3 and 6 h after treatment. In a second experiment, slow-coconut oil implants alone or containing naphthalene (doses of 10 and 50 mg/kg) were i.p. located and fish sacrificed 1, 3 and 5 days after treatment. Levels of dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), serotonin (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) and noradrenaline (NA) were measured in several brain regions by HPLC. The results show that short-term naphthalene increases DA and 5-HT contents in hypothalamus and telencephalon, but differentially alter contents of the acid metabolites. Implants with naphthalene reduced DA content in hypothalamus and preoptic region but increased in telencephalon. 5-HT metabolism was decreased in hypothalamus, preoptic region, pituitary and brain stem after 3 to 6 days of treatment. In addition, the levels of NA were increased in hypothalamus and telencephalon after acute treatment and in hypothalamus and preoptic area after several days of exposure to naphthalene. These data suggest that brain neurotransmitter systems are sensitive to polycyclic aromatic hydrocarbons and could represent a target of the naphtbalene-induced neuroendocrine disruption. (c) 2006 Elsevier Inc. All rights reserved.
Original languageEnglish
JournalComparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology
Volume144
Issue number2
Pages (from-to)173-183
ISSN1532-0456
DOIs
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • BIOCHEMISTRY
  • ENDOCRINOLOGY
  • TOXICOLOGY
  • ZOOLOGY
  • POLYCYCLIC AROMATIC-HYDROCARBONS
  • ANGUILLA-ANGUILLA L.
  • ERYTHROCYTIC NUCLEAR ABNORMALITIES
  • FLOUNDER PLATICHTHYS-FLESUS
  • CHARR SALVELINUS-ALPINUS
  • BETA-NAPHTHOFLAVONE
  • ATLANTIC CROAKER
  • POLYCHLORINATED-BIPHENYLS
  • SEROTONERGIC ACTIVITY
  • ENDOCRINE DISRUPTORS
  • brain neurotransmitters
  • dopamine
  • monoamines
  • naphthalene
  • noradrenaline
  • polycyclic aromatic hydrocarbons
  • serotonin
  • trout
  • 3,4-Dihydroxyphenylacetic Acid
  • Animals
  • Brain
  • Dopamine
  • Hydroxyindoleacetic Acid
  • Naphthalenes
  • Norepinephrine
  • Oncorhynchus mykiss
  • Serotonin
  • Water Pollutants, Chemical
  • Brain neurotransmitters
  • Monoamines
  • Naphthalene
  • Noradrenaline
  • Polycyclic aromatic hydrocarbons
  • Trout
  • Biochemistry
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Pharmacology
  • 3,4 dihydroxyphenylacetic acid
  • 5 hydroxyindoleacetic acid
  • coconut oil
  • endocrine disruptor
  • noradrenalin
  • polycyclic aromatic hydrocarbon
  • vegetable oil
  • animal experiment
  • animal tissue
  • article
  • brain region
  • brain stem
  • hypophysis
  • hypothalamus
  • monoaminergic system
  • neurotransmission
  • nonhuman
  • preoptic area
  • priority journal
  • telencephalon
  • Salmonidae
  • Pisces Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Osteichthyes [85206] Oncorhynchus mykiss species rainbow trout common immature
  • 17-beta-estradiol 50-28-2
  • 3,4-dihydroxyphenylacetic acid DOPAC
  • 5-hydroxyindoleacetic acid 54-16-0
  • dopamine 51-61-6
  • naphthalene 91-20-3 expression toxicity
  • noradrenaline 51-41-2
  • plasmatic cortisol
  • serotonin 50-67-9
  • 10060, Biochemistry studies - General
  • 10064, Biochemistry studies - Proteins, peptides and amino acids
  • 10067, Biochemistry studies - Sterols and steroids
  • 17002, Endocrine - General
  • 17020, Endocrine - Neuroendocrinology
  • 20504, Nervous system - Physiology and biochemistry
  • 22501, Toxicology - General and methods
  • Neural Coordination
  • brain nervous system
  • brain stem nervous system
  • endocrine gland endocrine system
  • hypothalamus nervous system
  • telencephalon nervous system
  • HPLC high performance liquid chromatography laboratory techniques, chromatographic techniques
  • Biochemistry and Molecular Biophysics
  • Nervous System
  • Toxicology

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