Effects of 14-day oral low dose selenium nanoparticles and selenite in rat—as determined by metabolite pattern determination

Niels Hadrup, Katrin Löschner, Kasper Skov, Gitte Ravn-Haren, Erik Huusfeldt Larsen, Alicja Mortensen, Henrik R. Lam, Henrik Lauritz Frandsen

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Abstract

Selenium (Se) is an essential element with a small difference between physiological and toxic doses. To provide more effective and safe Se dosing regimens, as compared to dosing with ionic selenium, nanoparticle formulations have been developed. However, due to the nano-formulation, unexpected toxic effects may occur. We used metabolite pattern determination in urine to investigate biological and/or toxic effects in rats administered nanoparticles and for comparison included ionic selenium at an equimolar dose in the form of sodium selenite. Low doses of 10 and 100 fold the recommended human high level were employed to study the effects at borderline toxicity. Evaluations of all significantly changed putative metabolites, showed that Se nanoparticles and sodium selenite induced similar dose dependent changes of the metabolite pattern. Putative identified metabolites included increased decenedioic acid and hydroxydecanedioic acid for both Se formulations whereas dipeptides were only increased for selenite. These effects could reflect altered fatty acid and protein metabolism, respectively.
Original languageEnglish
Article numbere2601
JournalPeerJ
Volume4
Number of pages14
ISSN2167-8359
DOIs
Publication statusPublished - 2016

Keywords

  • Food Science and Technology
  • Toxicology
  • Pharmacology
  • Metabolic Sciences
  • Selenium
  • Nanoparticle
  • Metabolomic pattern recognition

Cite this

Hadrup, Niels ; Löschner, Katrin ; Skov, Kasper ; Ravn-Haren, Gitte ; Larsen, Erik Huusfeldt ; Mortensen, Alicja ; Lam, Henrik R. ; Frandsen, Henrik Lauritz. / Effects of 14-day oral low dose selenium nanoparticles and selenite in rat—as determined by metabolite pattern determination. In: PeerJ. 2016 ; Vol. 4.
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abstract = "Selenium (Se) is an essential element with a small difference between physiological and toxic doses. To provide more effective and safe Se dosing regimens, as compared to dosing with ionic selenium, nanoparticle formulations have been developed. However, due to the nano-formulation, unexpected toxic effects may occur. We used metabolite pattern determination in urine to investigate biological and/or toxic effects in rats administered nanoparticles and for comparison included ionic selenium at an equimolar dose in the form of sodium selenite. Low doses of 10 and 100 fold the recommended human high level were employed to study the effects at borderline toxicity. Evaluations of all significantly changed putative metabolites, showed that Se nanoparticles and sodium selenite induced similar dose dependent changes of the metabolite pattern. Putative identified metabolites included increased decenedioic acid and hydroxydecanedioic acid for both Se formulations whereas dipeptides were only increased for selenite. These effects could reflect altered fatty acid and protein metabolism, respectively.",
keywords = "Food Science and Technology, Toxicology, Pharmacology, Metabolic Sciences, Selenium, Nanoparticle, Metabolomic pattern recognition",
author = "Niels Hadrup and Katrin L{\"o}schner and Kasper Skov and Gitte Ravn-Haren and Larsen, {Erik Huusfeldt} and Alicja Mortensen and Lam, {Henrik R.} and Frandsen, {Henrik Lauritz}",
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Effects of 14-day oral low dose selenium nanoparticles and selenite in rat—as determined by metabolite pattern determination. / Hadrup, Niels; Löschner, Katrin; Skov, Kasper; Ravn-Haren, Gitte; Larsen, Erik Huusfeldt; Mortensen, Alicja; Lam, Henrik R.; Frandsen, Henrik Lauritz.

In: PeerJ, Vol. 4, e2601, 2016.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Effects of 14-day oral low dose selenium nanoparticles and selenite in rat—as determined by metabolite pattern determination

AU - Hadrup, Niels

AU - Löschner, Katrin

AU - Skov, Kasper

AU - Ravn-Haren, Gitte

AU - Larsen, Erik Huusfeldt

AU - Mortensen, Alicja

AU - Lam, Henrik R.

AU - Frandsen, Henrik Lauritz

PY - 2016

Y1 - 2016

N2 - Selenium (Se) is an essential element with a small difference between physiological and toxic doses. To provide more effective and safe Se dosing regimens, as compared to dosing with ionic selenium, nanoparticle formulations have been developed. However, due to the nano-formulation, unexpected toxic effects may occur. We used metabolite pattern determination in urine to investigate biological and/or toxic effects in rats administered nanoparticles and for comparison included ionic selenium at an equimolar dose in the form of sodium selenite. Low doses of 10 and 100 fold the recommended human high level were employed to study the effects at borderline toxicity. Evaluations of all significantly changed putative metabolites, showed that Se nanoparticles and sodium selenite induced similar dose dependent changes of the metabolite pattern. Putative identified metabolites included increased decenedioic acid and hydroxydecanedioic acid for both Se formulations whereas dipeptides were only increased for selenite. These effects could reflect altered fatty acid and protein metabolism, respectively.

AB - Selenium (Se) is an essential element with a small difference between physiological and toxic doses. To provide more effective and safe Se dosing regimens, as compared to dosing with ionic selenium, nanoparticle formulations have been developed. However, due to the nano-formulation, unexpected toxic effects may occur. We used metabolite pattern determination in urine to investigate biological and/or toxic effects in rats administered nanoparticles and for comparison included ionic selenium at an equimolar dose in the form of sodium selenite. Low doses of 10 and 100 fold the recommended human high level were employed to study the effects at borderline toxicity. Evaluations of all significantly changed putative metabolites, showed that Se nanoparticles and sodium selenite induced similar dose dependent changes of the metabolite pattern. Putative identified metabolites included increased decenedioic acid and hydroxydecanedioic acid for both Se formulations whereas dipeptides were only increased for selenite. These effects could reflect altered fatty acid and protein metabolism, respectively.

KW - Food Science and Technology

KW - Toxicology

KW - Pharmacology

KW - Metabolic Sciences

KW - Selenium

KW - Nanoparticle

KW - Metabolomic pattern recognition

U2 - 10.7717/peerj.2601

DO - 10.7717/peerj.2601

M3 - Journal article

C2 - 27781177

VL - 4

JO - PeerJ

JF - PeerJ

SN - 2167-8359

M1 - e2601

ER -