Effect of tetracycline treatment regimens on antibiotic resistance gene selection over time in nursery pigs

Kaare Græsbøll, Inge Larsen, Julie Clasen, Anna Camilla Birkegård, Jens Peter Nielsen, Lasse Engbo Christiansen, John Elmerdahl Olsen, Øystein Angen, Anders Folkesson*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

24 Downloads (Pure)

Abstract

Background
The majority of antimicrobials given during the production of pigs are given to nursery pigs. The influence of antimicrobial use on the levels of antimicrobial resistant (AMR) genes is important to quantify to be able to assess the impact of resistance on the food chain and risk to human and animal health.
Results
This study investigated the response on the levels of nine AMR genes to five different treatment strategies with oxytetracycline, and the dynamics of gene abundance over time by following 1167 pigs from five different farms in Denmark.
The results showed no significant difference between treatments and an increase in abundance for the efflux pump encoding tet(A) gene and the genes encoding the ribosomal protection proteins tet(O) and tet(W) tetracycline resistant genes following treatment, while tet(M) showed no response to treatment. However, it was also observed that the levels of tet(O), tet(W), and ermB in some farms would drift more over time compared to a single treatment-course with antibiotic.
Conclusion
This study underlines the large variation in AMR levels under natural conditions and the need for increased investigation of the complex interactions of antimicrobial treatment and other environmental and managerial practices in swine production on AMR gene abundance.
Original languageEnglish
Article number269
JournalBMC Microbiology
Volume19
Number of pages10
ISSN1471-2180
DOIs
Publication statusPublished - 2019

Keywords

  • Oxytetracycline
  • Resistance genes
  • qPCR
  • Nursery pigs

Cite this

Græsbøll, Kaare ; Larsen, Inge ; Clasen, Julie ; Birkegård, Anna Camilla ; Nielsen, Jens Peter ; Christiansen, Lasse Engbo ; Olsen, John Elmerdahl ; Angen, Øystein ; Folkesson, Anders. / Effect of tetracycline treatment regimens on antibiotic resistance gene selection over time in nursery pigs. In: BMC Microbiology. 2019 ; Vol. 19.
@article{0cf208098c3347849c980a8c7051e25e,
title = "Effect of tetracycline treatment regimens on antibiotic resistance gene selection over time in nursery pigs",
abstract = "BackgroundThe majority of antimicrobials given during the production of pigs are given to nursery pigs. The influence of antimicrobial use on the levels of antimicrobial resistant (AMR) genes is important to quantify to be able to assess the impact of resistance on the food chain and risk to human and animal health.ResultsThis study investigated the response on the levels of nine AMR genes to five different treatment strategies with oxytetracycline, and the dynamics of gene abundance over time by following 1167 pigs from five different farms in Denmark.The results showed no significant difference between treatments and an increase in abundance for the efflux pump encoding tet(A) gene and the genes encoding the ribosomal protection proteins tet(O) and tet(W) tetracycline resistant genes following treatment, while tet(M) showed no response to treatment. However, it was also observed that the levels of tet(O), tet(W), and ermB in some farms would drift more over time compared to a single treatment-course with antibiotic.ConclusionThis study underlines the large variation in AMR levels under natural conditions and the need for increased investigation of the complex interactions of antimicrobial treatment and other environmental and managerial practices in swine production on AMR gene abundance.",
keywords = "Oxytetracycline, Resistance genes, qPCR, Nursery pigs",
author = "Kaare Gr{\ae}sb{\o}ll and Inge Larsen and Julie Clasen and Birkeg{\aa}rd, {Anna Camilla} and Nielsen, {Jens Peter} and Christiansen, {Lasse Engbo} and Olsen, {John Elmerdahl} and {\O}ystein Angen and Anders Folkesson",
year = "2019",
doi = "10.1186/s12866-019-1619-z",
language = "English",
volume = "19",
journal = "B M C Microbiology",
issn = "1471-2180",
publisher = "BioMed Central Ltd.",

}

Effect of tetracycline treatment regimens on antibiotic resistance gene selection over time in nursery pigs. / Græsbøll, Kaare; Larsen, Inge; Clasen, Julie; Birkegård, Anna Camilla; Nielsen, Jens Peter; Christiansen, Lasse Engbo; Olsen, John Elmerdahl; Angen, Øystein; Folkesson, Anders.

In: BMC Microbiology, Vol. 19, 269, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Effect of tetracycline treatment regimens on antibiotic resistance gene selection over time in nursery pigs

AU - Græsbøll, Kaare

AU - Larsen, Inge

AU - Clasen, Julie

AU - Birkegård, Anna Camilla

AU - Nielsen, Jens Peter

AU - Christiansen, Lasse Engbo

AU - Olsen, John Elmerdahl

AU - Angen, Øystein

AU - Folkesson, Anders

PY - 2019

Y1 - 2019

N2 - BackgroundThe majority of antimicrobials given during the production of pigs are given to nursery pigs. The influence of antimicrobial use on the levels of antimicrobial resistant (AMR) genes is important to quantify to be able to assess the impact of resistance on the food chain and risk to human and animal health.ResultsThis study investigated the response on the levels of nine AMR genes to five different treatment strategies with oxytetracycline, and the dynamics of gene abundance over time by following 1167 pigs from five different farms in Denmark.The results showed no significant difference between treatments and an increase in abundance for the efflux pump encoding tet(A) gene and the genes encoding the ribosomal protection proteins tet(O) and tet(W) tetracycline resistant genes following treatment, while tet(M) showed no response to treatment. However, it was also observed that the levels of tet(O), tet(W), and ermB in some farms would drift more over time compared to a single treatment-course with antibiotic.ConclusionThis study underlines the large variation in AMR levels under natural conditions and the need for increased investigation of the complex interactions of antimicrobial treatment and other environmental and managerial practices in swine production on AMR gene abundance.

AB - BackgroundThe majority of antimicrobials given during the production of pigs are given to nursery pigs. The influence of antimicrobial use on the levels of antimicrobial resistant (AMR) genes is important to quantify to be able to assess the impact of resistance on the food chain and risk to human and animal health.ResultsThis study investigated the response on the levels of nine AMR genes to five different treatment strategies with oxytetracycline, and the dynamics of gene abundance over time by following 1167 pigs from five different farms in Denmark.The results showed no significant difference between treatments and an increase in abundance for the efflux pump encoding tet(A) gene and the genes encoding the ribosomal protection proteins tet(O) and tet(W) tetracycline resistant genes following treatment, while tet(M) showed no response to treatment. However, it was also observed that the levels of tet(O), tet(W), and ermB in some farms would drift more over time compared to a single treatment-course with antibiotic.ConclusionThis study underlines the large variation in AMR levels under natural conditions and the need for increased investigation of the complex interactions of antimicrobial treatment and other environmental and managerial practices in swine production on AMR gene abundance.

KW - Oxytetracycline

KW - Resistance genes

KW - qPCR

KW - Nursery pigs

U2 - 10.1186/s12866-019-1619-z

DO - 10.1186/s12866-019-1619-z

M3 - Journal article

C2 - 31791243

VL - 19

JO - B M C Microbiology

JF - B M C Microbiology

SN - 1471-2180

M1 - 269

ER -