Effect of pulsed electric field on the germination of barley seeds

Katarzyna Dymek, Petr Dejmek, Valentina Panarese, António A. Vicente, Lars Wadsö, Christine Finnie, Federico Gómez Galindo

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    This study explores metabolic responses of germinating barley seeds upon the application of pulsed electric fields (PEF). Malting barley seeds were steeped in aerated water for 24 h and PEF-treated at varying voltages (0 (control), 110, 160, 240, 320, 400 and 480 V). The seeds were then allowed to finish germination in saturated air. It is shown that exposure of germinating barley to PEF affects radicle emergence without significantly affecting the seeds’ gross metabolic activity, as quantified by isothermal calorimetry. An exploration of protein 2-DE profiles of both the embryo and the starchy endosperm showed that, at the studied time scale, no significant changes were found in proteins present at concentrations higher than the detection limit. However, western blotting demonstrated that α-amylase concentration decreases in the PEF-treated seeds.
    Original languageEnglish
    JournalL W T- Food Science and Technology
    Volume47
    Issue number1
    Pages (from-to)161-166
    DOIs
    Publication statusPublished - 2012

    Keywords

    • Radical emergence
    • Isothermal calorimetry
    • a-Amylase

    Fingerprint Dive into the research topics of 'Effect of pulsed electric field on the germination of barley seeds'. Together they form a unique fingerprint.

    Cite this