Effect of plant proteins and crystalline amino acid supplementation on postprandial plasma amino acid profiles and metabolic response in rainbow trout (Oncorhynchus mykiss)

Marine Rolland, Bodil Katrine Larsen, Jørgen Holm, Anne Johanne Tang Dalsgaard, Peter Vilhelm Skov

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The use of aquafeeds formulated with plant protein sources supplemented with crystalline amino acids (CAAs) is believed to influence amino acid (AA) uptake patterns and AA metabolic fate. Oxygen consumption and ammonia excretion rates were measured in rainbow trout (468.5 +/- A 86.5 g) force fed 0.75 % of their body mass with a diet based on either (1) fish meal (FM), (2) pea protein concentrate (PPC), or (3) pea protein concentrate supplemented with histidine, lysine, methionine and threonine (PPC+) to mimic FM AA profile. The specific dynamic action and nitrogen quotient (NQ) were calculated for 48 h of the postprandial period. In parallel, plasma AA concentrations were measured in blood samples withdrawn from the caudal vein before and then 2, 4, 6, 8, 12, 20, 32 and 48 h after feed administration. The unbalanced diet PPC had a significantly higher NQ compared to FM (0.29 +/- A 0.09 and 0.18 +/- A 0.04, respectively), and plasma profiles of essential AAs reflected the dietary deficiencies. Supplementation with CAA in diet PPC+ resulted in an intermediary NQ (0.21 +/- A 0.04) and significantly affected plasma AA profiles, presenting greater and faster rises followed by sharp decreases compared to FM. The strongest effect was observed for methionine, presenting threefold higher concentrations at peak time for PPC+ compared to FM (297.0 +/- A 77.0 and 131.8 +/- A 39.0 nmol ml(-1), respectively). The differences in AA availability and metabolic profile in the pea diets compared to the FM diet were believed to be caused by an unbalanced dietary AA profile and CAA supplementation, rather than inclusion of plant protein concentrate.
Original languageEnglish
JournalAquaculture International
Volume23
Issue number4
Pages (from-to)1071-1087
Number of pages17
ISSN0967-6120
DOIs
Publication statusPublished - 2015

Keywords

  • FISHERIES
  • SALMON SALMO-SALAR
  • CATFISH ICTALURUS-PUNCTATUS
  • CARP CYPRINUS-CARPIO
  • GROWTH-PERFORMANCE
  • DYNAMIC ACTION
  • CHANNEL CATFISH
  • FISH-MEAL
  • GENE-EXPRESSION
  • BOUND LYSINE
  • L-METHIONINE
  • Essential amino acid
  • Non-essential amino acid
  • Ammonia excretion
  • Respirometry
  • SDA
  • HASH(0x41106c8)

Cite this

@article{05be34ebb3f74bf38ea897ffcb5eae10,
title = "Effect of plant proteins and crystalline amino acid supplementation on postprandial plasma amino acid profiles and metabolic response in rainbow trout (Oncorhynchus mykiss)",
abstract = "The use of aquafeeds formulated with plant protein sources supplemented with crystalline amino acids (CAAs) is believed to influence amino acid (AA) uptake patterns and AA metabolic fate. Oxygen consumption and ammonia excretion rates were measured in rainbow trout (468.5 +/- A 86.5 g) force fed 0.75 {\%} of their body mass with a diet based on either (1) fish meal (FM), (2) pea protein concentrate (PPC), or (3) pea protein concentrate supplemented with histidine, lysine, methionine and threonine (PPC+) to mimic FM AA profile. The specific dynamic action and nitrogen quotient (NQ) were calculated for 48 h of the postprandial period. In parallel, plasma AA concentrations were measured in blood samples withdrawn from the caudal vein before and then 2, 4, 6, 8, 12, 20, 32 and 48 h after feed administration. The unbalanced diet PPC had a significantly higher NQ compared to FM (0.29 +/- A 0.09 and 0.18 +/- A 0.04, respectively), and plasma profiles of essential AAs reflected the dietary deficiencies. Supplementation with CAA in diet PPC+ resulted in an intermediary NQ (0.21 +/- A 0.04) and significantly affected plasma AA profiles, presenting greater and faster rises followed by sharp decreases compared to FM. The strongest effect was observed for methionine, presenting threefold higher concentrations at peak time for PPC+ compared to FM (297.0 +/- A 77.0 and 131.8 +/- A 39.0 nmol ml(-1), respectively). The differences in AA availability and metabolic profile in the pea diets compared to the FM diet were believed to be caused by an unbalanced dietary AA profile and CAA supplementation, rather than inclusion of plant protein concentrate.",
keywords = "FISHERIES, SALMON SALMO-SALAR, CATFISH ICTALURUS-PUNCTATUS, CARP CYPRINUS-CARPIO, GROWTH-PERFORMANCE, DYNAMIC ACTION, CHANNEL CATFISH, FISH-MEAL, GENE-EXPRESSION, BOUND LYSINE, L-METHIONINE, Essential amino acid, Non-essential amino acid, Ammonia excretion, Respirometry, SDA, HASH(0x41106c8)",
author = "Marine Rolland and Larsen, {Bodil Katrine} and J{\o}rgen Holm and Dalsgaard, {Anne Johanne Tang} and Skov, {Peter Vilhelm}",
year = "2015",
doi = "10.1007/s10499-014-9865-4",
language = "English",
volume = "23",
pages = "1071--1087",
journal = "Aquaculture International",
issn = "0967-6120",
publisher = "Springer Netherlands",
number = "4",

}

Effect of plant proteins and crystalline amino acid supplementation on postprandial plasma amino acid profiles and metabolic response in rainbow trout (Oncorhynchus mykiss). / Rolland, Marine; Larsen, Bodil Katrine; Holm, Jørgen; Dalsgaard, Anne Johanne Tang; Skov, Peter Vilhelm.

In: Aquaculture International, Vol. 23, No. 4, 2015, p. 1071-1087.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Effect of plant proteins and crystalline amino acid supplementation on postprandial plasma amino acid profiles and metabolic response in rainbow trout (Oncorhynchus mykiss)

AU - Rolland, Marine

AU - Larsen, Bodil Katrine

AU - Holm, Jørgen

AU - Dalsgaard, Anne Johanne Tang

AU - Skov, Peter Vilhelm

PY - 2015

Y1 - 2015

N2 - The use of aquafeeds formulated with plant protein sources supplemented with crystalline amino acids (CAAs) is believed to influence amino acid (AA) uptake patterns and AA metabolic fate. Oxygen consumption and ammonia excretion rates were measured in rainbow trout (468.5 +/- A 86.5 g) force fed 0.75 % of their body mass with a diet based on either (1) fish meal (FM), (2) pea protein concentrate (PPC), or (3) pea protein concentrate supplemented with histidine, lysine, methionine and threonine (PPC+) to mimic FM AA profile. The specific dynamic action and nitrogen quotient (NQ) were calculated for 48 h of the postprandial period. In parallel, plasma AA concentrations were measured in blood samples withdrawn from the caudal vein before and then 2, 4, 6, 8, 12, 20, 32 and 48 h after feed administration. The unbalanced diet PPC had a significantly higher NQ compared to FM (0.29 +/- A 0.09 and 0.18 +/- A 0.04, respectively), and plasma profiles of essential AAs reflected the dietary deficiencies. Supplementation with CAA in diet PPC+ resulted in an intermediary NQ (0.21 +/- A 0.04) and significantly affected plasma AA profiles, presenting greater and faster rises followed by sharp decreases compared to FM. The strongest effect was observed for methionine, presenting threefold higher concentrations at peak time for PPC+ compared to FM (297.0 +/- A 77.0 and 131.8 +/- A 39.0 nmol ml(-1), respectively). The differences in AA availability and metabolic profile in the pea diets compared to the FM diet were believed to be caused by an unbalanced dietary AA profile and CAA supplementation, rather than inclusion of plant protein concentrate.

AB - The use of aquafeeds formulated with plant protein sources supplemented with crystalline amino acids (CAAs) is believed to influence amino acid (AA) uptake patterns and AA metabolic fate. Oxygen consumption and ammonia excretion rates were measured in rainbow trout (468.5 +/- A 86.5 g) force fed 0.75 % of their body mass with a diet based on either (1) fish meal (FM), (2) pea protein concentrate (PPC), or (3) pea protein concentrate supplemented with histidine, lysine, methionine and threonine (PPC+) to mimic FM AA profile. The specific dynamic action and nitrogen quotient (NQ) were calculated for 48 h of the postprandial period. In parallel, plasma AA concentrations were measured in blood samples withdrawn from the caudal vein before and then 2, 4, 6, 8, 12, 20, 32 and 48 h after feed administration. The unbalanced diet PPC had a significantly higher NQ compared to FM (0.29 +/- A 0.09 and 0.18 +/- A 0.04, respectively), and plasma profiles of essential AAs reflected the dietary deficiencies. Supplementation with CAA in diet PPC+ resulted in an intermediary NQ (0.21 +/- A 0.04) and significantly affected plasma AA profiles, presenting greater and faster rises followed by sharp decreases compared to FM. The strongest effect was observed for methionine, presenting threefold higher concentrations at peak time for PPC+ compared to FM (297.0 +/- A 77.0 and 131.8 +/- A 39.0 nmol ml(-1), respectively). The differences in AA availability and metabolic profile in the pea diets compared to the FM diet were believed to be caused by an unbalanced dietary AA profile and CAA supplementation, rather than inclusion of plant protein concentrate.

KW - FISHERIES

KW - SALMON SALMO-SALAR

KW - CATFISH ICTALURUS-PUNCTATUS

KW - CARP CYPRINUS-CARPIO

KW - GROWTH-PERFORMANCE

KW - DYNAMIC ACTION

KW - CHANNEL CATFISH

KW - FISH-MEAL

KW - GENE-EXPRESSION

KW - BOUND LYSINE

KW - L-METHIONINE

KW - Essential amino acid

KW - Non-essential amino acid

KW - Ammonia excretion

KW - Respirometry

KW - SDA

KW - HASH(0x41106c8)

U2 - 10.1007/s10499-014-9865-4

DO - 10.1007/s10499-014-9865-4

M3 - Journal article

VL - 23

SP - 1071

EP - 1087

JO - Aquaculture International

JF - Aquaculture International

SN - 0967-6120

IS - 4

ER -