Effect of pH and activated charcoal adsorption on hemicellulosic hydrolysate detoxification for xylitol production

Solange I. Mussatto, Júlio C. Santos, Inês Conceicao Roberto

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Biotechnological conversion of xylose into xylitol using hydrolysates obtained from the hemicellulosic fraction of lignocellulosic materials is compromised by the presence of compounds released or formed during the hydrolysis process, some of them being toxic to microorganisms. In order to improve the bioconversion of these hydrolysates it is necessary to find methods to reduce their toxicity. In the present work, rice straw hemicellulosic hydrolysate was treated by six different procedures (all of them involving pH adjustment, with or without activated charcoal adsorption), before being used as a fermentation medium for xylitol production. The most effective method of treatment was to increase the initial pH (0.4) to 2.0 using solid NaOH, followed by the addition of activated charcoal (25 g kg−1) and increase in the pH to 6.5 using solid NaOH. Lignin degradation products were the most inhibitory compounds present in the hydrolysate; their removal was selective and strongly dependent on the pH employed in the treatment. The highest yield of xylitol was 0.72 g g−1 xylose, with a productivity of 0.55 g dm−3 h−1.
Original languageEnglish
JournalJournal of Chemical Technology and Biotechnology
Volume79
Issue number6
Pages (from-to)590-596
ISSN0268-2575
DOIs
Publication statusPublished - 2004
Externally publishedYes

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