Effect of nitrogen source and acclimatization on specific growth rates of microalgae determined by a high-throughput in vivo microplate autofluorescence method

Mike Podevin, Davide De Francisci, Susan Løvstad Holdt, Irini Angelidaki

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Specific growth rates (SGR) of freshwater algaespecies (Chlorella vulgaris, Auxenochlorella protothecoides,and Chlorella sorokiniana) and the marine speciesNannochloropsis oculata on various nitrogen sources (ammoniumcarbonate, ammonium chloride, sodium nitrate, andurea) could be determined by in vivo chlorophyll-a autofluorescence.These preferences could be determined before large pH changes occurred in the media, with no significant difference(P>0.05) between buffered and non-buffered media. In all algal species, acclimatization was observed with no significant difference (P>0.05) between SGRs of the second and third cultivations. ANOVA of SGRs in the acclimatized second and third cultivations revealed preferences for nitrogen sources among most of the algae; C. vulgaris preferred sodiumnitrate over other nitrogen sources, A. protothecoides adapted to urea after no growth in the first cultivation, and the SGRs of N. oculata showed an aversion for sodium nitrateover other nitrogen sources (P<0.05).
Original languageEnglish
JournalJournal of Applied Phycology
Volume27
Issue number4
Pages (from-to)1415-1423
Number of pages9
ISSN0921-8971
DOIs
Publication statusPublished - 2015

Keywords

  • Adaptation
  • Auxenochlorella
  • Chlorella
  • Nannochloropsis
  • pH changes
  • Industrial wastewater

Cite this

@article{0a00102e74664b6e8cb2fea7c2462fa5,
title = "Effect of nitrogen source and acclimatization on specific growth rates of microalgae determined by a high-throughput in vivo microplate autofluorescence method",
abstract = "Specific growth rates (SGR) of freshwater algaespecies (Chlorella vulgaris, Auxenochlorella protothecoides,and Chlorella sorokiniana) and the marine speciesNannochloropsis oculata on various nitrogen sources (ammoniumcarbonate, ammonium chloride, sodium nitrate, andurea) could be determined by in vivo chlorophyll-a autofluorescence.These preferences could be determined before large pH changes occurred in the media, with no significant difference(P>0.05) between buffered and non-buffered media. In all algal species, acclimatization was observed with no significant difference (P>0.05) between SGRs of the second and third cultivations. ANOVA of SGRs in the acclimatized second and third cultivations revealed preferences for nitrogen sources among most of the algae; C. vulgaris preferred sodiumnitrate over other nitrogen sources, A. protothecoides adapted to urea after no growth in the first cultivation, and the SGRs of N. oculata showed an aversion for sodium nitrateover other nitrogen sources (P<0.05).",
keywords = "Adaptation, Auxenochlorella, Chlorella , Nannochloropsis, pH changes, Industrial wastewater",
author = "Mike Podevin and {De Francisci}, Davide and Holdt, {Susan L{\o}vstad} and Irini Angelidaki",
year = "2015",
doi = "10.1007/s10811-014-0468-2",
language = "English",
volume = "27",
pages = "1415--1423",
journal = "Journal of Applied Phycology",
issn = "0921-8971",
publisher = "Springer",
number = "4",

}

Effect of nitrogen source and acclimatization on specific growth rates of microalgae determined by a high-throughput in vivo microplate autofluorescence method. / Podevin, Mike; De Francisci, Davide; Holdt, Susan Løvstad; Angelidaki, Irini.

In: Journal of Applied Phycology, Vol. 27, No. 4, 2015, p. 1415-1423.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Effect of nitrogen source and acclimatization on specific growth rates of microalgae determined by a high-throughput in vivo microplate autofluorescence method

AU - Podevin, Mike

AU - De Francisci, Davide

AU - Holdt, Susan Løvstad

AU - Angelidaki, Irini

PY - 2015

Y1 - 2015

N2 - Specific growth rates (SGR) of freshwater algaespecies (Chlorella vulgaris, Auxenochlorella protothecoides,and Chlorella sorokiniana) and the marine speciesNannochloropsis oculata on various nitrogen sources (ammoniumcarbonate, ammonium chloride, sodium nitrate, andurea) could be determined by in vivo chlorophyll-a autofluorescence.These preferences could be determined before large pH changes occurred in the media, with no significant difference(P>0.05) between buffered and non-buffered media. In all algal species, acclimatization was observed with no significant difference (P>0.05) between SGRs of the second and third cultivations. ANOVA of SGRs in the acclimatized second and third cultivations revealed preferences for nitrogen sources among most of the algae; C. vulgaris preferred sodiumnitrate over other nitrogen sources, A. protothecoides adapted to urea after no growth in the first cultivation, and the SGRs of N. oculata showed an aversion for sodium nitrateover other nitrogen sources (P<0.05).

AB - Specific growth rates (SGR) of freshwater algaespecies (Chlorella vulgaris, Auxenochlorella protothecoides,and Chlorella sorokiniana) and the marine speciesNannochloropsis oculata on various nitrogen sources (ammoniumcarbonate, ammonium chloride, sodium nitrate, andurea) could be determined by in vivo chlorophyll-a autofluorescence.These preferences could be determined before large pH changes occurred in the media, with no significant difference(P>0.05) between buffered and non-buffered media. In all algal species, acclimatization was observed with no significant difference (P>0.05) between SGRs of the second and third cultivations. ANOVA of SGRs in the acclimatized second and third cultivations revealed preferences for nitrogen sources among most of the algae; C. vulgaris preferred sodiumnitrate over other nitrogen sources, A. protothecoides adapted to urea after no growth in the first cultivation, and the SGRs of N. oculata showed an aversion for sodium nitrateover other nitrogen sources (P<0.05).

KW - Adaptation

KW - Auxenochlorella

KW - Chlorella

KW - Nannochloropsis

KW - pH changes

KW - Industrial wastewater

U2 - 10.1007/s10811-014-0468-2

DO - 10.1007/s10811-014-0468-2

M3 - Journal article

VL - 27

SP - 1415

EP - 1423

JO - Journal of Applied Phycology

JF - Journal of Applied Phycology

SN - 0921-8971

IS - 4

ER -