Effect of micro-aeration and inoculum type on the biodegradation of lignocellulosic substrate

Panagiotis Tsapekos, Panagiotis Kougias, S. A. Vasileiou, G. Lyberatos, Irini Angelidaki

Research output: Contribution to journalJournal articleResearchpeer-review


The effect of various micro-aeration strategies on the anaerobic digestion (AD) of wheat straw was thoroughly examined using a mixture of inocula, containing compost and well digested sludge from biogas plant. The aim was to determine the most efficient oxygen load, pulse repetition and treatment duration, resulting in the highest methane production. The oxygen load had the largest impact on the biodegradability of straw, among the examined variables. More specifically, a micro-aeration intensity of 10mLO2/gVS was identified as the critical threshold above which the AD performance was more susceptible to instability. The highest enhancement in biogas production was achieved by injecting 5mLO2/gVS for a consecutive 3-day treatment period, presenting a 7.2% increase compared to the untreated wheat straw. Nevertheless, the results from optimisation case study indicated a higher increase of 9% by injecting 7.3mLO2/gVS, distributed in 2 pulses during a slightly shorter treatment period (i.e. 47h).
Original languageEnglish
JournalBioresource Technology
Pages (from-to)246-253
Number of pages8
Publication statusPublished - 2017


  • Anaerobic digestion
  • Methane yield
  • Micro-aeration
  • Response surface methodology
  • Wheat straw

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