Effect of melittin on water diffusion and membrane structure in DMPC lipid bilayers

Z. N. Buck, J. Torres, A. Miskowiec, E. Mamontov, H. Kaiser, F. Y. Hansen, H. Taub*, M. Tyagi, L. Collins, K. W. Herwig

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Quasielastic neutron scattering (QENS) is well suited for studying the dynamics of water in proximity to supported membranes whose structure can be characterized by atomic force microscopy (AFM). Here we use QENS to investigate the effect of an adsorbed peptide (melittin) on water diffusion near a single-supported zwitterionic membrane (DMPC). Measurements of the incoherent elastic neutron intensity as a function of temperature provide evidence of bulk-like water freezing onto the melittin, which AFM images indicate coalesces into peptide-lipid domains as the peptide concentration increases. Analysis of the QENS spectra indicates that, at sufficiently high melittin concentrations, a water component diffusing more slowly than bulk-like water first freezes onto the bound melittin.
Original languageEnglish
Article number18002
JournalEpl
Volume123
Number of pages8
ISSN0295-5075
DOIs
Publication statusPublished - 2018

Cite this

Buck, Z. N., Torres, J., Miskowiec, A., Mamontov, E., Kaiser, H., Hansen, F. Y., ... Herwig, K. W. (2018). Effect of melittin on water diffusion and membrane structure in DMPC lipid bilayers. Epl, 123, [18002]. https://doi.org/10.1209/0295-5075/123/18002
Buck, Z. N. ; Torres, J. ; Miskowiec, A. ; Mamontov, E. ; Kaiser, H. ; Hansen, F. Y. ; Taub, H. ; Tyagi, M. ; Collins, L. ; Herwig, K. W. / Effect of melittin on water diffusion and membrane structure in DMPC lipid bilayers. In: Epl. 2018 ; Vol. 123.
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title = "Effect of melittin on water diffusion and membrane structure in DMPC lipid bilayers",
abstract = "Quasielastic neutron scattering (QENS) is well suited for studying the dynamics of water in proximity to supported membranes whose structure can be characterized by atomic force microscopy (AFM). Here we use QENS to investigate the effect of an adsorbed peptide (melittin) on water diffusion near a single-supported zwitterionic membrane (DMPC). Measurements of the incoherent elastic neutron intensity as a function of temperature provide evidence of bulk-like water freezing onto the melittin, which AFM images indicate coalesces into peptide-lipid domains as the peptide concentration increases. Analysis of the QENS spectra indicates that, at sufficiently high melittin concentrations, a water component diffusing more slowly than bulk-like water first freezes onto the bound melittin.",
author = "Buck, {Z. N.} and J. Torres and A. Miskowiec and E. Mamontov and H. Kaiser and Hansen, {F. Y.} and H. Taub and M. Tyagi and L. Collins and Herwig, {K. W.}",
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language = "English",
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Buck, ZN, Torres, J, Miskowiec, A, Mamontov, E, Kaiser, H, Hansen, FY, Taub, H, Tyagi, M, Collins, L & Herwig, KW 2018, 'Effect of melittin on water diffusion and membrane structure in DMPC lipid bilayers', Epl, vol. 123, 18002. https://doi.org/10.1209/0295-5075/123/18002

Effect of melittin on water diffusion and membrane structure in DMPC lipid bilayers. / Buck, Z. N.; Torres, J.; Miskowiec, A.; Mamontov, E.; Kaiser, H.; Hansen, F. Y.; Taub, H.; Tyagi, M.; Collins, L.; Herwig, K. W.

In: Epl, Vol. 123, 18002, 2018.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Effect of melittin on water diffusion and membrane structure in DMPC lipid bilayers

AU - Buck, Z. N.

AU - Torres, J.

AU - Miskowiec, A.

AU - Mamontov, E.

AU - Kaiser, H.

AU - Hansen, F. Y.

AU - Taub, H.

AU - Tyagi, M.

AU - Collins, L.

AU - Herwig, K. W.

PY - 2018

Y1 - 2018

N2 - Quasielastic neutron scattering (QENS) is well suited for studying the dynamics of water in proximity to supported membranes whose structure can be characterized by atomic force microscopy (AFM). Here we use QENS to investigate the effect of an adsorbed peptide (melittin) on water diffusion near a single-supported zwitterionic membrane (DMPC). Measurements of the incoherent elastic neutron intensity as a function of temperature provide evidence of bulk-like water freezing onto the melittin, which AFM images indicate coalesces into peptide-lipid domains as the peptide concentration increases. Analysis of the QENS spectra indicates that, at sufficiently high melittin concentrations, a water component diffusing more slowly than bulk-like water first freezes onto the bound melittin.

AB - Quasielastic neutron scattering (QENS) is well suited for studying the dynamics of water in proximity to supported membranes whose structure can be characterized by atomic force microscopy (AFM). Here we use QENS to investigate the effect of an adsorbed peptide (melittin) on water diffusion near a single-supported zwitterionic membrane (DMPC). Measurements of the incoherent elastic neutron intensity as a function of temperature provide evidence of bulk-like water freezing onto the melittin, which AFM images indicate coalesces into peptide-lipid domains as the peptide concentration increases. Analysis of the QENS spectra indicates that, at sufficiently high melittin concentrations, a water component diffusing more slowly than bulk-like water first freezes onto the bound melittin.

U2 - 10.1209/0295-5075/123/18002

DO - 10.1209/0295-5075/123/18002

M3 - Journal article

VL - 123

JO - EPL

JF - EPL

SN - 0295-5075

M1 - 18002

ER -