Effect of Laser and LED on Enzymatic Production of Ceramide

An enzyme (Phospholipase C Type I from Clostridium perfringens) was exposed to 0-810J/cm2 of energy using laser light at wavelengths 808 nm, 532nm, 1064 nm, and 1342 nm and two LED light sources at wavelengths 810 nm and 640 nm. Enzyme responses were evaluated by measuring ceramide concentration using HPTLC (high performance thin-layer chromatography) at 0.5, 1, 2, 3, 4, 6, 17, 24 h after irradiation. The duration of effect was evaluated from the experimental data. The results show that enzyme activity can be increased by using both laser and LED sources whose wavelength is located within a certain range. The effect depends on the energy and wavelength of the light. The increase in enzyme activity continued for about 4 h after irradiation. This study shows that the duration of irradiation should be included as one of the main laser parameters when reporting on the effects of laser irradiation on enzymes. We also find that laser sources and LED sources have the same effect on enzyme activity if the wavelength and absorbed energy are equal.