Effect of hydraulic retention time on the modelling and optimization of joint 1,3 PDO and BuA production from 2G glycerol in a chemostat process

Cristiano Varrone*, Ioannis V. Skiadas, Hariklia N. Gavala

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

This study investigated the possibility to perform statistical optimization of an enriched mixed microbial consortium, MMC, fed in continuous with a highly inhibiting 2G glycerol, for the joint production of 1,3 propanediol (1,3 PDO) and butyric acid (BuA). Key variables taken into consideration were HRT, pH and feed concentration. The optimized process reached a glycerol consumption rate of 137 g/L/d and a maximum predicted production rate of 82.61 g/L/d and 21.13 g/L/d for 1,3 PDO and BuA, respectively. This corresponded to a 2.2 and 1.9-fold increase compared to the non-optimized process. The model was able to satisfactorily predict the joint maximum production of 1,3 PDO and BuA (22.6 and 8.1 g/L respectively) of the steady state also in non-sterile conditions, implying thus that MMC can be a robust and reliable biological production platform.
Original languageEnglish
JournalChemical Engineering Journal
Volume347
Pages (from-to)525-534
ISSN1385-8947
DOIs
Publication statusPublished - 2018

Keywords

  • HRT
  • Continuous fermentation
  • Statistical optimization
  • Joint production
  • Mixed microbial consortia
  • 2G crude glycerol

Cite this

@article{7c70e16d46d24967a3067dc2d35bad26,
title = "Effect of hydraulic retention time on the modelling and optimization of joint 1,3 PDO and BuA production from 2G glycerol in a chemostat process",
abstract = "This study investigated the possibility to perform statistical optimization of an enriched mixed microbial consortium, MMC, fed in continuous with a highly inhibiting 2G glycerol, for the joint production of 1,3 propanediol (1,3 PDO) and butyric acid (BuA). Key variables taken into consideration were HRT, pH and feed concentration. The optimized process reached a glycerol consumption rate of 137 g/L/d and a maximum predicted production rate of 82.61 g/L/d and 21.13 g/L/d for 1,3 PDO and BuA, respectively. This corresponded to a 2.2 and 1.9-fold increase compared to the non-optimized process. The model was able to satisfactorily predict the joint maximum production of 1,3 PDO and BuA (22.6 and 8.1 g/L respectively) of the steady state also in non-sterile conditions, implying thus that MMC can be a robust and reliable biological production platform.",
keywords = "HRT, Continuous fermentation, Statistical optimization, Joint production, Mixed microbial consortia, 2G crude glycerol",
author = "Cristiano Varrone and Skiadas, {Ioannis V.} and Gavala, {Hariklia N.}",
year = "2018",
doi = "10.1016/j.cej.2018.04.071",
language = "English",
volume = "347",
pages = "525--534",
journal = "Biochemical Engineering Journal",
issn = "1369-703X",
publisher = "Elsevier",

}

Effect of hydraulic retention time on the modelling and optimization of joint 1,3 PDO and BuA production from 2G glycerol in a chemostat process. / Varrone, Cristiano; Skiadas, Ioannis V.; Gavala, Hariklia N.

In: Chemical Engineering Journal, Vol. 347, 2018, p. 525-534.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Effect of hydraulic retention time on the modelling and optimization of joint 1,3 PDO and BuA production from 2G glycerol in a chemostat process

AU - Varrone, Cristiano

AU - Skiadas, Ioannis V.

AU - Gavala, Hariklia N.

PY - 2018

Y1 - 2018

N2 - This study investigated the possibility to perform statistical optimization of an enriched mixed microbial consortium, MMC, fed in continuous with a highly inhibiting 2G glycerol, for the joint production of 1,3 propanediol (1,3 PDO) and butyric acid (BuA). Key variables taken into consideration were HRT, pH and feed concentration. The optimized process reached a glycerol consumption rate of 137 g/L/d and a maximum predicted production rate of 82.61 g/L/d and 21.13 g/L/d for 1,3 PDO and BuA, respectively. This corresponded to a 2.2 and 1.9-fold increase compared to the non-optimized process. The model was able to satisfactorily predict the joint maximum production of 1,3 PDO and BuA (22.6 and 8.1 g/L respectively) of the steady state also in non-sterile conditions, implying thus that MMC can be a robust and reliable biological production platform.

AB - This study investigated the possibility to perform statistical optimization of an enriched mixed microbial consortium, MMC, fed in continuous with a highly inhibiting 2G glycerol, for the joint production of 1,3 propanediol (1,3 PDO) and butyric acid (BuA). Key variables taken into consideration were HRT, pH and feed concentration. The optimized process reached a glycerol consumption rate of 137 g/L/d and a maximum predicted production rate of 82.61 g/L/d and 21.13 g/L/d for 1,3 PDO and BuA, respectively. This corresponded to a 2.2 and 1.9-fold increase compared to the non-optimized process. The model was able to satisfactorily predict the joint maximum production of 1,3 PDO and BuA (22.6 and 8.1 g/L respectively) of the steady state also in non-sterile conditions, implying thus that MMC can be a robust and reliable biological production platform.

KW - HRT

KW - Continuous fermentation

KW - Statistical optimization

KW - Joint production

KW - Mixed microbial consortia

KW - 2G crude glycerol

U2 - 10.1016/j.cej.2018.04.071

DO - 10.1016/j.cej.2018.04.071

M3 - Journal article

VL - 347

SP - 525

EP - 534

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

SN - 1369-703X

ER -