Effect of highly bioaccumulated polychlorinated biphenyl congeners on estrogen and androgen receptor activity

E.C. Bonefeld-Jørgensen, H. R. Andersen, T.H. Rasmussen, Anne Vinggaard

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Polychlorinated biphenyls (PCBs) are ubiquitous environmental persistent contaminants giving rise to potential health hazard. Some PCBs exert dioxin-like activities mediated through the aryl hydrocarbon receptor. Although reports on interaction with other nuclear receptors are sparce, some congeners are hypothesized to possess endocrine disruptive potential. Here we present evidence that the three PCBs most abundant in biological extracts, 2,2',3'4,4',5-hexachlorobiphenyl (PCB # 138), 2,2',4,4',5,5'-hexachlorobiphenyl (PCB # 153), and 2,2',3,4,4',5,5'-heptachlorobiphenyl (PCB # 180) have pleiotropic effects on the estrogen- and androgen-receptor. In MCF-7 cells a slightly increased cell proliferation was observed at low concentrations (1-10 nM) in cells co-treated with 0.01 nM 17 beta -Estradiol. whereas the compounds inhibited cell growth significantly at 1 and 10 muM. In reporter gene (ERE-tk-CAT) analysis the three congeners exhibited a significantly estrogen receptor-ligand mediated decrease of the chloramphenicol transferase activity in both control and 10 nM 17 beta -estradiol induced MCF-7 cells. In addition, PCB # 138 elicited a dose-dependent antagonistic effect on androgen receptor activity in transiently co-transfected Chinese Hamster Ovary cells with an IC50, of 6.2 muM. In summary, this study indicate that the di-ortho, multiple-chloro substituted biphenyls, PCB # 138, PCB # 153 and PCB # 180, can compete with the binding of the natural ligand to two nuclear receptors and thus possess the ability to interfere with sexual hormone regulated processes.
Original languageEnglish
JournalToxicology
Volume158
Issue number3
Pages (from-to)141-153
ISSN0300-483X
DOIs
Publication statusPublished - 2001

Keywords

  • AH-receptor
  • androgen receptor
  • breast cancer cells
  • TCDD
  • PCB
  • estrogen receptor

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