Effect of deletion of chitin synthase genes on mycelial morphology and culture viscosity in Aspergillus oryzae

Christian Müller, K. Hansen, Peter Szabo, Jens Nielsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The objective of this study was to quantify the effect of disrupting two chitin synthases, chsB and csmA, on the morphology and rheology during batch cultivation of Aspergillus oryzae. The rheological properties were characterized in batch cultivations at different biomass concentrations (from 3.4-22.5 g kg(-1) biomass) and the power-law model adequately described the rheological properties. In the cultivations there were pellets, clumps, and freely dispersed hyphal elements. The different morphological fractions were quantified using image analysis. The apparent viscosity of the fermentation broth was significantly affected by the biomass concentration, the morphology, and also by pH. The chsB disruption strain had lower consistency index K values for all biomass concentrations investigated, which is a desirable trait for industrial Aspergillus fermentations. (C) 2003 Wiley Periodicals, Inc.
Original languageEnglish
JournalBiotechnology and Bioengineering (Print)
Volume81
Issue number5
Pages (from-to)525-534
ISSN0006-3592
DOIs
Publication statusPublished - 2003

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