Effect of bioaugmentation on long-term biodegradation of diesel/biodiesel blends in soil microcosms

Marta Woźniak-Karczewska, Piotr Lisiecki, Wojciech Białas, Mikołaj Owsianiak, Agnieszka Piotrowska-Cyplik, Łukasz Wolko, Łukasz Ławniczak, Hermann J. Heipieper, Tony Gutierrez, Łukasz Chrzanowski*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We studied long-term (64.5 weeks) biodegradation of diesel fuel, diesel/biodiesel blends (B10-B90) and biodiesel fuels in urban soil microcosms containing indigenous microorganisms, or indigenous microorganisms augmented with a hydrocarbon-degrading bacterial community. Mineralization extent (mmol of CO2 per day) of B10-B30 blends was smaller compared with diesel fuel at both short- (28 days) and long-term (109 days), and increased with biodiesel content. Priming with hydrocarbon degraders accelerated mineralization in the short-term (by up to 140%), with highest influence using blends with lower biodiesel content, but did not significantly influence kinetics and mineralization extent in the long-term. Although the biodiesel fraction was degraded completely within 64.5 weeks, 3–12% of the total aromatic and aliphatic hydrocarbons remained in the microcosms. Barcoded 16S rRNA gene MiSeq sequencing analysis revealed a significant effect of blend type on the community structure, with a marked enrichment of Sphingobacteriia and Actinobacteria classes. However, no significant influence was determined in the long-term, suggesting that the inoculated bacterial community may not have survived. Our findings show that biodiesel is preferentially degraded in urban soil and suggest that the value of bioaugmentation for bioremediating biodiesel fuels with hydrocarbon-degrading bacteria is limited to short-term exposures to lower (B10-B30) blends.

Original languageEnglish
JournalScience of the Total Environment
Volume671
Pages (from-to)948-958
Number of pages11
ISSN0048-9697
DOIs
Publication statusPublished - 2019

Keywords

  • Bacterial community
  • Fuel blends
  • Hydrocarbons
  • Mineralization
  • MiSeq sequencing

Cite this

Woźniak-Karczewska, M., Lisiecki, P., Białas, W., Owsianiak, M., Piotrowska-Cyplik, A., Wolko, Ł., ... Chrzanowski, Ł. (2019). Effect of bioaugmentation on long-term biodegradation of diesel/biodiesel blends in soil microcosms. Science of the Total Environment, 671, 948-958. https://doi.org/10.1016/j.scitotenv.2019.03.431
Woźniak-Karczewska, Marta ; Lisiecki, Piotr ; Białas, Wojciech ; Owsianiak, Mikołaj ; Piotrowska-Cyplik, Agnieszka ; Wolko, Łukasz ; Ławniczak, Łukasz ; Heipieper, Hermann J. ; Gutierrez, Tony ; Chrzanowski, Łukasz. / Effect of bioaugmentation on long-term biodegradation of diesel/biodiesel blends in soil microcosms. In: Science of the Total Environment. 2019 ; Vol. 671. pp. 948-958.
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title = "Effect of bioaugmentation on long-term biodegradation of diesel/biodiesel blends in soil microcosms",
abstract = "We studied long-term (64.5 weeks) biodegradation of diesel fuel, diesel/biodiesel blends (B10-B90) and biodiesel fuels in urban soil microcosms containing indigenous microorganisms, or indigenous microorganisms augmented with a hydrocarbon-degrading bacterial community. Mineralization extent (mmol of CO2 per day) of B10-B30 blends was smaller compared with diesel fuel at both short- (28 days) and long-term (109 days), and increased with biodiesel content. Priming with hydrocarbon degraders accelerated mineralization in the short-term (by up to 140{\%}), with highest influence using blends with lower biodiesel content, but did not significantly influence kinetics and mineralization extent in the long-term. Although the biodiesel fraction was degraded completely within 64.5 weeks, 3–12{\%} of the total aromatic and aliphatic hydrocarbons remained in the microcosms. Barcoded 16S rRNA gene MiSeq sequencing analysis revealed a significant effect of blend type on the community structure, with a marked enrichment of Sphingobacteriia and Actinobacteria classes. However, no significant influence was determined in the long-term, suggesting that the inoculated bacterial community may not have survived. Our findings show that biodiesel is preferentially degraded in urban soil and suggest that the value of bioaugmentation for bioremediating biodiesel fuels with hydrocarbon-degrading bacteria is limited to short-term exposures to lower (B10-B30) blends.",
keywords = "Bacterial community, Fuel blends, Hydrocarbons, Mineralization, MiSeq sequencing",
author = "Marta Woźniak-Karczewska and Piotr Lisiecki and Wojciech Białas and Mikołaj Owsianiak and Agnieszka Piotrowska-Cyplik and Łukasz Wolko and Łukasz Ławniczak and Heipieper, {Hermann J.} and Tony Gutierrez and Łukasz Chrzanowski",
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pages = "948--958",
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Woźniak-Karczewska, M, Lisiecki, P, Białas, W, Owsianiak, M, Piotrowska-Cyplik, A, Wolko, Ł, Ławniczak, Ł, Heipieper, HJ, Gutierrez, T & Chrzanowski, Ł 2019, 'Effect of bioaugmentation on long-term biodegradation of diesel/biodiesel blends in soil microcosms', Science of the Total Environment, vol. 671, pp. 948-958. https://doi.org/10.1016/j.scitotenv.2019.03.431

Effect of bioaugmentation on long-term biodegradation of diesel/biodiesel blends in soil microcosms. / Woźniak-Karczewska, Marta; Lisiecki, Piotr; Białas, Wojciech; Owsianiak, Mikołaj; Piotrowska-Cyplik, Agnieszka; Wolko, Łukasz; Ławniczak, Łukasz; Heipieper, Hermann J.; Gutierrez, Tony; Chrzanowski, Łukasz.

In: Science of the Total Environment, Vol. 671, 2019, p. 948-958.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Effect of bioaugmentation on long-term biodegradation of diesel/biodiesel blends in soil microcosms

AU - Woźniak-Karczewska, Marta

AU - Lisiecki, Piotr

AU - Białas, Wojciech

AU - Owsianiak, Mikołaj

AU - Piotrowska-Cyplik, Agnieszka

AU - Wolko, Łukasz

AU - Ławniczak, Łukasz

AU - Heipieper, Hermann J.

AU - Gutierrez, Tony

AU - Chrzanowski, Łukasz

PY - 2019

Y1 - 2019

N2 - We studied long-term (64.5 weeks) biodegradation of diesel fuel, diesel/biodiesel blends (B10-B90) and biodiesel fuels in urban soil microcosms containing indigenous microorganisms, or indigenous microorganisms augmented with a hydrocarbon-degrading bacterial community. Mineralization extent (mmol of CO2 per day) of B10-B30 blends was smaller compared with diesel fuel at both short- (28 days) and long-term (109 days), and increased with biodiesel content. Priming with hydrocarbon degraders accelerated mineralization in the short-term (by up to 140%), with highest influence using blends with lower biodiesel content, but did not significantly influence kinetics and mineralization extent in the long-term. Although the biodiesel fraction was degraded completely within 64.5 weeks, 3–12% of the total aromatic and aliphatic hydrocarbons remained in the microcosms. Barcoded 16S rRNA gene MiSeq sequencing analysis revealed a significant effect of blend type on the community structure, with a marked enrichment of Sphingobacteriia and Actinobacteria classes. However, no significant influence was determined in the long-term, suggesting that the inoculated bacterial community may not have survived. Our findings show that biodiesel is preferentially degraded in urban soil and suggest that the value of bioaugmentation for bioremediating biodiesel fuels with hydrocarbon-degrading bacteria is limited to short-term exposures to lower (B10-B30) blends.

AB - We studied long-term (64.5 weeks) biodegradation of diesel fuel, diesel/biodiesel blends (B10-B90) and biodiesel fuels in urban soil microcosms containing indigenous microorganisms, or indigenous microorganisms augmented with a hydrocarbon-degrading bacterial community. Mineralization extent (mmol of CO2 per day) of B10-B30 blends was smaller compared with diesel fuel at both short- (28 days) and long-term (109 days), and increased with biodiesel content. Priming with hydrocarbon degraders accelerated mineralization in the short-term (by up to 140%), with highest influence using blends with lower biodiesel content, but did not significantly influence kinetics and mineralization extent in the long-term. Although the biodiesel fraction was degraded completely within 64.5 weeks, 3–12% of the total aromatic and aliphatic hydrocarbons remained in the microcosms. Barcoded 16S rRNA gene MiSeq sequencing analysis revealed a significant effect of blend type on the community structure, with a marked enrichment of Sphingobacteriia and Actinobacteria classes. However, no significant influence was determined in the long-term, suggesting that the inoculated bacterial community may not have survived. Our findings show that biodiesel is preferentially degraded in urban soil and suggest that the value of bioaugmentation for bioremediating biodiesel fuels with hydrocarbon-degrading bacteria is limited to short-term exposures to lower (B10-B30) blends.

KW - Bacterial community

KW - Fuel blends

KW - Hydrocarbons

KW - Mineralization

KW - MiSeq sequencing

U2 - 10.1016/j.scitotenv.2019.03.431

DO - 10.1016/j.scitotenv.2019.03.431

M3 - Journal article

AN - SCOPUS:85063687396

VL - 671

SP - 948

EP - 958

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

ER -