Effect of apple pectin on gut microbiota - qPCR in applied microbiology

Anders Bergström, Andrea Wilcks, Morten Poulsen, Lars Ove Dragsted, Tine Rask Licht

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Abstract

This study was part of the large European project ISAFRUIT aiming to reveal the biological explanations for the epidemiologically well-established health effects of fruits. The objective was to identify effects of apple and apple product consumption on the composition of the cecal microbial community in rats, as well as on a number of cecal parameters, which could be influenced by a changed microbiota. Principal Component Analysis (PCA) of cecal microbiota profiles obtained by PCR-DGGE targeting bacterial 16S rRNA genes showed an effect of whole apples in a long-term feeding study (14 weeks), while no effects of apple juice, purée or pomace on microbial composition in cecum were observed. Administration of pectin derived from apples resulted in considerable changes of these DGGE profiles. A 2-fold increase in the activity of beta-glucuronidase was observed in animals fed with pectin (7% in the diet) for four weeks, as compared to control animals (P
Original languageEnglish
Publication date2009
Publication statusPublished - 2009
Event4th International qPCR Symposium & Industrial Exhibition & Application Workshop - Freising, Germany
Duration: 9 Mar 200913 Mar 2009
Conference number: 4
http://www.qpcr2009.net/

Conference

Conference4th International qPCR Symposium & Industrial Exhibition & Application Workshop
Number4
CountryGermany
CityFreising
Period09/03/200913/03/2009
Internet address

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