Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism

Kristian H Mikkelsen, Morten Frost, Martin Iain Bahl, Tine Rask Licht, Ulrich S. Jensen, Jacob Rosenberg, Oluf Pedersen, Torben Hansen, Jens F. Rehfeld, Jens J. Holst, Tina Vilsbøll, Filip K. Knop

Research output: Contribution to journalJournal articleResearchpeer-review

382 Downloads (Pure)

Abstract

The gut microbiota has been designated as an active regulator of glucose metabolism and metabolic phenotype in a number of animal and human observational studies. We evaluated the effect of removing as many bacteria as possible by antibiotics on postprandial physiology in healthy humans. Meal tests with measurements of postprandial glucose tolerance and postprandial release of insulin and gut hormones were performed before, immediately after and 6 weeks after a 4-day, broad-spectrum, per oral antibiotic cocktail (vancomycin 500 mg, gentamycin 40 mg and meropenem 500 mg once-daily) in a group of 12 lean and glucose tolerant males. Faecal samples were collected for culture-based assessment of changes in gut microbiota composition. Acute and dramatic reductions in the abundance of a representative set of gut bacteria was seen immediately following the antibiotic course, but no changes in postprandial glucose tolerance, insulin secretion or plasma lipid concentrations were found. Apart from an acute and reversible increase in peptide YY secretion, no changes were observed in postprandial gut hormone release. As evaluated by selective cultivation of gut bacteria, a broad-spectrum 4-day antibiotics course with vancomycin, gentamycin and meropenem induced shifts in gut microbiota composition that had no clinically relevant short or long-term effects on metabolic variables in healthy glucose-tolerant males. clinicaltrials.gov NCT01633762.
Original languageEnglish
Article numbere0142352
JournalP L o S One
Volume10
Issue number11
Number of pages14
ISSN1932-6203
DOIs
Publication statusPublished - 2015

Fingerprint Dive into the research topics of 'Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism'. Together they form a unique fingerprint.

Cite this