Effect of ammonia on hydrogenotrophic methanogens and syntrophic acetate oxidizing bacteria

Han Wang, Ioannis Fotidis, Irini Angelidaki

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    Substrates that contain high ammonia levels can cause inhibition on anaerobic digestion process and unstable biogas production. The aim of the current study was to assess the effects of different ammonia levels on pure strains of (syntrophic acetate oxidizing) SAO bacteria and hydrogenotrophic methanogens. Two pure strains of hydrogenotrophic methanogens (i.e: Methanoculleus bourgensis and Methanoculleus thermophiles) and two pure strains of SAO bacteria (i.e: Tepidanaerobacter acetatoxydans and Thermacetogenium phaeum) were inoculated under four different ammonia (0.26, 3, 5 and 7g NH4+-N/L) and free ammonia levels (Mesophilic: 3.31, 38.2, 63.68 and 89.15 g NH3-N/L. Thermophilic: 8.48, 97.82,163.03 and 228.24 g NH3-N/L)(Westerholm, et al., 2011; Satoshi, et al., 2000; Jacob, et al., 1997). The results indicated that both T. acetatoxydans and T. phaeum were more sensitive to high ammonia levels compared to the hydrogenotrophic methanogens tested. Thus, it seems that hydrogenotrophic methanogens could be equally, if not more, tolerant to high ammonia levels compared to SAO bacteria.
    Original languageEnglish
    Title of host publicationBook of Abstracts. DTU's Sustain Conference 2015
    Number of pages1
    Place of PublicationLyngby
    PublisherTechnical University of Denmark
    Publication date2015
    Article numberR-15
    Publication statusPublished - 2015
    EventDTU Sustain Conference 2015 - DTU, Lyngby, Denmark
    Duration: 17 Dec 201517 Dec 2015


    ConferenceDTU Sustain Conference 2015
    Internet address


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