Effect of ammonia on hydrogenotrophic methanogens and syntrophic acetate oxidizing bacteria

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Abstract

Substrates that contain high ammonia levels can cause inhibition on anaerobic digestion process and unstable biogas production. The aim of the current study was to assess the effects of different ammonia levels on pure strains of (syntrophic acetate oxidizing) SAO bacteria and hydrogenotrophic methanogens. Two pure strains of hydrogenotrophic methanogens (i.e: Methanoculleus bourgensis and Methanoculleus thermophiles) and two pure strains of SAO bacteria (i.e: Tepidanaerobacter acetatoxydans and Thermacetogenium phaeum) were inoculated under four different ammonia (0.26, 3, 5 and 7g NH4+-N/L) and free ammonia levels (Mesophilic: 3.31, 38.2, 63.68 and 89.15 g NH3-N/L. Thermophilic: 8.48, 97.82,163.03 and 228.24 g NH3-N/L)(Westerholm, et al., 2011; Satoshi, et al., 2000; Jacob, et al., 1997). The results indicated that both T. acetatoxydans and T. phaeum were more sensitive to high ammonia levels compared to the hydrogenotrophic methanogens tested. Thus, it seems that hydrogenotrophic methanogens could be equally, if not more, tolerant to high ammonia levels compared to SAO bacteria.
Original languageEnglish
Title of host publicationBook of Abstracts. DTU's Sustain Conference 2015
Number of pages1
Place of PublicationLyngby
PublisherTechnical University of Denmark
Publication date2015
Article numberR-15
Publication statusPublished - 2015
EventDTU Sustain Conference 2015 - DTU, Lyngby, Denmark
Duration: 17 Dec 201517 Dec 2015
http://www.sustain.dtu.dk/About/Conference-2015

Conference

ConferenceDTU Sustain Conference 2015
LocationDTU
CountryDenmark
CityLyngby
Period17/12/201517/12/2015
Internet address

Cite this

Wang, H., Fotidis, I., & Angelidaki, I. (2015). Effect of ammonia on hydrogenotrophic methanogens and syntrophic acetate oxidizing bacteria. In Book of Abstracts. DTU's Sustain Conference 2015 [R-15] Technical University of Denmark.