Effect of alginate size, mannuronic/guluronic acid content and pH on particle size, thermodynamics and composition of complexes with β-lactoglobulin

Emil G.P. Stender, Sanaullah Khan, Richard Ipsen, Finn Madsen, Per Hägglund, Maher Abou Hachem, Kristoffer Almdal, Peter Westh, Birte Svensson*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

172 Downloads (Pure)

Abstract

Alginate is an anionic polysaccharide capable of forming insoluble particles with proteins. Hence, alginate has potential as a protein carrier. However, the role of physical properties of the polysaccharide, such as degree of polymerization (DPn) and mannuronic/guluronic acid ratio, remains to be fully explored. Particle formation of a high and a low molar mass alginate (ALG) with β-lactoglobulin (BLG) at pH 2-8 depends on the average DPn (HMW-ALG: 1.59·103; LMW-ALG: 0.23·103) and the mannuronic/guluronic acid ratio (1.0; 0.6) as supported by using ManA6 and GulA6 as models. Dynamic light scattering (DLS) showed that particles of BLG with either of the two ALGs have essentially the same hydrodynamic diameter (D H) at pH 3 and 2, while at pH 4 particles of LMW-ALG/BLG have larger D H than of HMW-ALG/BLG. At pH 5-8 no significant particle formation was observed. ManA6 did not form insoluble particles at pH 2-8, while GulA6 formed insoluble particles, albeit only at pH 4. K d was approximately 10-fold higher for LMW-ALG/BLG than HMW-ALG/BLG and 3 orders of magnitude higher for an alginate trisaccharide/BLG complexation as determined by isothermal titration calorimetry (ITC). The alginate trisaccharide did not form insoluble particles with BLG at pH 3 and 4, though interaction still occurred. δH app and molar stoichiometry of BLG in the complexes with the two ALGs differed by a factor of 7, as did their DPn, which thus affected the interaction strength, but not the BLG content. At pH 4 the BLG content doubled in the particle due to BLG dimerization. The findings emphasize the importance of DPn, mannuronic/guluronic acid ratio and pH in formulations containing alginate/whey protein particles.

Original languageEnglish
JournalFood Hydrocolloids
Volume75
Pages (from-to)157-163
ISSN0268-005X
DOIs
Publication statusPublished - 2018

Cite this

@article{c251633b6f094300a0e9e5c301f2fa26,
title = "Effect of alginate size, mannuronic/guluronic acid content and pH on particle size, thermodynamics and composition of complexes with β-lactoglobulin",
abstract = "Alginate is an anionic polysaccharide capable of forming insoluble particles with proteins. Hence, alginate has potential as a protein carrier. However, the role of physical properties of the polysaccharide, such as degree of polymerization (DPn) and mannuronic/guluronic acid ratio, remains to be fully explored. Particle formation of a high and a low molar mass alginate (ALG) with β-lactoglobulin (BLG) at pH 2-8 depends on the average DPn (HMW-ALG: 1.59·103; LMW-ALG: 0.23·103) and the mannuronic/guluronic acid ratio (1.0; 0.6) as supported by using ManA6 and GulA6 as models. Dynamic light scattering (DLS) showed that particles of BLG with either of the two ALGs have essentially the same hydrodynamic diameter (D H) at pH 3 and 2, while at pH 4 particles of LMW-ALG/BLG have larger D H than of HMW-ALG/BLG. At pH 5-8 no significant particle formation was observed. ManA6 did not form insoluble particles at pH 2-8, while GulA6 formed insoluble particles, albeit only at pH 4. K d was approximately 10-fold higher for LMW-ALG/BLG than HMW-ALG/BLG and 3 orders of magnitude higher for an alginate trisaccharide/BLG complexation as determined by isothermal titration calorimetry (ITC). The alginate trisaccharide did not form insoluble particles with BLG at pH 3 and 4, though interaction still occurred. δH app and molar stoichiometry of BLG in the complexes with the two ALGs differed by a factor of 7, as did their DPn, which thus affected the interaction strength, but not the BLG content. At pH 4 the BLG content doubled in the particle due to BLG dimerization. The findings emphasize the importance of DPn, mannuronic/guluronic acid ratio and pH in formulations containing alginate/whey protein particles.",
author = "Stender, {Emil G.P.} and Sanaullah Khan and Richard Ipsen and Finn Madsen and Per H{\"a}gglund and Hachem, {Maher Abou} and Kristoffer Almdal and Peter Westh and Birte Svensson",
year = "2018",
doi = "10.1016/j.foodhyd.2017.09.001",
language = "English",
volume = "75",
pages = "157--163",
journal = "Food Hydrocolloids",
issn = "0268-005X",
publisher = "Elsevier",

}

Effect of alginate size, mannuronic/guluronic acid content and pH on particle size, thermodynamics and composition of complexes with β-lactoglobulin. / Stender, Emil G.P.; Khan, Sanaullah; Ipsen, Richard; Madsen, Finn; Hägglund, Per; Hachem, Maher Abou; Almdal, Kristoffer; Westh, Peter; Svensson, Birte.

In: Food Hydrocolloids, Vol. 75, 2018, p. 157-163.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Effect of alginate size, mannuronic/guluronic acid content and pH on particle size, thermodynamics and composition of complexes with β-lactoglobulin

AU - Stender, Emil G.P.

AU - Khan, Sanaullah

AU - Ipsen, Richard

AU - Madsen, Finn

AU - Hägglund, Per

AU - Hachem, Maher Abou

AU - Almdal, Kristoffer

AU - Westh, Peter

AU - Svensson, Birte

PY - 2018

Y1 - 2018

N2 - Alginate is an anionic polysaccharide capable of forming insoluble particles with proteins. Hence, alginate has potential as a protein carrier. However, the role of physical properties of the polysaccharide, such as degree of polymerization (DPn) and mannuronic/guluronic acid ratio, remains to be fully explored. Particle formation of a high and a low molar mass alginate (ALG) with β-lactoglobulin (BLG) at pH 2-8 depends on the average DPn (HMW-ALG: 1.59·103; LMW-ALG: 0.23·103) and the mannuronic/guluronic acid ratio (1.0; 0.6) as supported by using ManA6 and GulA6 as models. Dynamic light scattering (DLS) showed that particles of BLG with either of the two ALGs have essentially the same hydrodynamic diameter (D H) at pH 3 and 2, while at pH 4 particles of LMW-ALG/BLG have larger D H than of HMW-ALG/BLG. At pH 5-8 no significant particle formation was observed. ManA6 did not form insoluble particles at pH 2-8, while GulA6 formed insoluble particles, albeit only at pH 4. K d was approximately 10-fold higher for LMW-ALG/BLG than HMW-ALG/BLG and 3 orders of magnitude higher for an alginate trisaccharide/BLG complexation as determined by isothermal titration calorimetry (ITC). The alginate trisaccharide did not form insoluble particles with BLG at pH 3 and 4, though interaction still occurred. δH app and molar stoichiometry of BLG in the complexes with the two ALGs differed by a factor of 7, as did their DPn, which thus affected the interaction strength, but not the BLG content. At pH 4 the BLG content doubled in the particle due to BLG dimerization. The findings emphasize the importance of DPn, mannuronic/guluronic acid ratio and pH in formulations containing alginate/whey protein particles.

AB - Alginate is an anionic polysaccharide capable of forming insoluble particles with proteins. Hence, alginate has potential as a protein carrier. However, the role of physical properties of the polysaccharide, such as degree of polymerization (DPn) and mannuronic/guluronic acid ratio, remains to be fully explored. Particle formation of a high and a low molar mass alginate (ALG) with β-lactoglobulin (BLG) at pH 2-8 depends on the average DPn (HMW-ALG: 1.59·103; LMW-ALG: 0.23·103) and the mannuronic/guluronic acid ratio (1.0; 0.6) as supported by using ManA6 and GulA6 as models. Dynamic light scattering (DLS) showed that particles of BLG with either of the two ALGs have essentially the same hydrodynamic diameter (D H) at pH 3 and 2, while at pH 4 particles of LMW-ALG/BLG have larger D H than of HMW-ALG/BLG. At pH 5-8 no significant particle formation was observed. ManA6 did not form insoluble particles at pH 2-8, while GulA6 formed insoluble particles, albeit only at pH 4. K d was approximately 10-fold higher for LMW-ALG/BLG than HMW-ALG/BLG and 3 orders of magnitude higher for an alginate trisaccharide/BLG complexation as determined by isothermal titration calorimetry (ITC). The alginate trisaccharide did not form insoluble particles with BLG at pH 3 and 4, though interaction still occurred. δH app and molar stoichiometry of BLG in the complexes with the two ALGs differed by a factor of 7, as did their DPn, which thus affected the interaction strength, but not the BLG content. At pH 4 the BLG content doubled in the particle due to BLG dimerization. The findings emphasize the importance of DPn, mannuronic/guluronic acid ratio and pH in formulations containing alginate/whey protein particles.

U2 - 10.1016/j.foodhyd.2017.09.001

DO - 10.1016/j.foodhyd.2017.09.001

M3 - Journal article

VL - 75

SP - 157

EP - 163

JO - Food Hydrocolloids

JF - Food Hydrocolloids

SN - 0268-005X

ER -